MTC week 8 Flashcards
what is the natural uncoupling protein, that causes heat in babies without shivering, called?
what is the natural uncoupling protein, that causes heat in babies without shivering, called?
Answer: thermogenin
the same amount of ATP is produced by substrate level phosphorylation in glycolysis as is produced in the TCA cycle?
True or False
the same amount of ATP is produced by substrate level phosphorylation in glycolysis as is produced in the TCA cycle?
Answer: true
how is lactate made?
how is lactate made?
Answer:
glycolysis -> 2 pyruvate –[lactate dehydrogenase rxn: 2NADH -> 2NAD+]–> 2 lactate (Lactic acid)
how is alcohol made?
how is alcohol made?
Answer:
glycolysis -> 2 pyruvate –[pyruvate dehydrogenase reaction: 2 CO2 leave]–> 2 acetaldehyde –[alcohol dehydrogenase reaction: 2NADH -> 2NAD+]–> 2 ethanol
what does Dinitrophenol (DNP) do in the ETC?
what does Dinitrophenol (DNP) do in the ETC?
Answer: it is an uncoupler that carries electrons across the inner mitochondrial membrane through simple diffusion-mediated transport. (destroys H+ gradient)
in a nutshell glycolysis, TCA, and ETC, all lead to the following reaction, so why not just do it straight without all the intermediate steps:
NADH + H+ + (1/2)O2 –> NAD+ + H2O
in a nutshell glycolysis, TCA, and ETC, all lead to the following reaction, so why not just do it straight without all the intermediate steps:
NADH + H+ + (1/2)O2 –> NAD+ + H2O
Answer: this reaction is not controllable; very exergonic
what does rotenone do?
where does it act?
What can bypass rotenone?
what does rotenone do?
where does it act?
What can bypass rotenone?
Answer:
- it inhibits the transfer of electrons from complex I to ubiquinone (Q)
- succinate can resume normal function.
what does Antimycin C do?
where does it act?
What can bypass it?
what does Antimycin C do?
where does it act?
What can bypass it?
Answer:
- inhibits transfer of electrons from Q to cytochrome C at complex III. Thus, inhibiting Oxygen consumption.
- Ascorbate can donate electrons directly to cytochrome C.
what chemicals inhibit the flow of electrons in complex IV?
hint: c.a.hs.cm
what chemicals inhibit the flow of electrons in complex IV?
Answer:
- Cyanide
- Azide
- H2S
- Carbon Monoxide
what chemical inhibits what in complex V (ATP synthase)?
what chemical inhibits what in complex V (ATP synthase)?
Answer: Oligomycin inhibits the passage of H+ through the ATP synthase
NADH = ? ATP FADH2 = ? ATP
NADH = 2.5 ATP FADH2 = 1.5 ATP
what is the poison that binds to oxaloacetate and makes a substance that cannot be metabolised by aconitase?
what is the overall result of this?
what is the poison that binds to oxaloacetate and makes a substance that cannot be metabolised by aconitase?
Answer: Fluroactete
what is the overall result of this?
Answer: TCA stops!
when is CO2 lost in the TCA?
when is CO2 lost in the TCA?
Answer:
- isocitrate dehydrogenase
- alpha-ketoglutarate dehydrogenase
what is chemiosmosis?
what is chemiosmosis?
Answer: H+ diffuse back into the mitochondrial matrix through a channel protein, ATP synthase.
how much ATP does a person hydrolyze in a day?
how much ATP does a person hydrolyze in a day?
Answer: 10^25 ATP molecules = 9 Kg of ATP
