MONOSPOT LATEX Flashcards

1
Q

Also known as “KISSING DISEASE”

A

INFECTIOUS MONONUCLEOSIS

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1
Q

Causative agent of infectious mononucleosis

A

EPSTEIN-BARR VIRUS

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2
Q

mode of transmission of IM

A
  • intimate contact
  • saliva
  • blood transfusion
  • semen
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3
Q

diagnosis of IM

A

Hematological and serological MTDS

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4
Q

what diseases can be diagnosed using the hematological method

A

lymphocytosis with
prominent atypical lymphocytes

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5
Q

what diseases can be diagnosed using the serological method

A

(+) heterophile and EBV antibodies

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6
Q

triad manifestation of IM that can last 1-4 weeks

A

Fever
pharyngitis,
cervical lymphadenopathy

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6
Q

EBV is also a cause of

A

Burkitt’s lymphoma
Nasopharyngeal carcinoma

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7
Q

Composed of a broad range of generally weak
antibodies induced by heterophile antigens

A

HETEROPHILE ANTIBODIES

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8
Q

The antibodies present in IM are:

A

EBV antibodies (Specifc)
Heterophile antibodies (Non-specifc)

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8
Q

A defnitive diagnosis can be established by

A

SEROLOGICAL ANTIBODY TESTING

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9
Q

Stimulated by one antigen and react with an entirely
unrelated surface antigen present on cells from
diferent mammalian species

A

HETEROPHILE ANTIBODIES

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10
Q

titer ratio of heterophile antibodies

A

Titer of 1:56

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10
Q

Heterophile antibodies (HAb) are made up usually of

A

IgM isotype

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11
Q

formed against Forssman antigens have the ability to agglutinate sheep RBCs

A

FORSSMAN ANTIBODIES

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11
Q

classic example of heterophile antigen, found on the surface of RBCs of many species

A

Glycolipid protein

11
Q

Simple titration of sheep cell agglutinins

A

PAUL-BUNNELL-DAVIDSOHN TEST

11
Q

Forssman antibodies are found in

A

normal serum
serum sickness

12
Q

what specimen is added to dilutions of patient’s
serum

A

Sheep RBCs

12
Q

PAUL-BUNNELL-DAVIDSOHN TEST is done with what specimen

A

inactivated serum

13
Q

the serum must be heated at what temp

A

56 degrees Celsius

14
Q

primary substrate of monospot test

A

equine erythrocytes

15
Q

what does monospot test detects against ebv infection

A

specifc heterophile antibodies

16
Q

Suspension of polystyrene latex particles coated
with ,,, from bovine red cell membranes.

A

highly purifed Paul-Bunnell antigen

17
result in heterogenous solution
CLUMPING
18
result in homogenous solution
NO CLUMPING
19
Monospot Latex Reagent that act as preservative
sodium azide 0.1%
20
Positive control is reactive/non-reactive with diluted human serum
reactive
21
negative control is reactive/non-reactive with diluted human serum
non-reactive
22
Qualitative immunochromatographic assay
TEST CASSETTE
23
what is the first reagent in test casette
Goat antihuman-IgM blue latex conjugate
24
Second reagent in test casette
Bovine erythrocyte extract
25
interpret Negative test
NO agglutination
25
the presence of agglutination indicates CLINICALLY SIGNIFICANT CONCENTRATION of infectious mononucleosis antibody in the sample.
qualitative technique
26
the appropriate titer will correspond to the highest dilution that still presents a clearly visible agglutination.
semiquantitative technique
27
interpret weak-positive result
Slight agglutination
27
interpret positive result
Agglutination