MONOSPOT LATEX Flashcards
Also known as “KISSING DISEASE”
INFECTIOUS MONONUCLEOSIS
Causative agent of infectious mononucleosis
EPSTEIN-BARR VIRUS
mode of transmission of IM
- intimate contact
- saliva
- blood transfusion
- semen
diagnosis of IM
Hematological and serological MTDS
what diseases can be diagnosed using the hematological method
lymphocytosis with
prominent atypical lymphocytes
what diseases can be diagnosed using the serological method
(+) heterophile and EBV antibodies
triad manifestation of IM that can last 1-4 weeks
Fever
pharyngitis,
cervical lymphadenopathy
EBV is also a cause of
Burkitt’s lymphoma
Nasopharyngeal carcinoma
Composed of a broad range of generally weak
antibodies induced by heterophile antigens
HETEROPHILE ANTIBODIES
The antibodies present in IM are:
EBV antibodies (Specifc)
Heterophile antibodies (Non-specifc)
A defnitive diagnosis can be established by
SEROLOGICAL ANTIBODY TESTING
Stimulated by one antigen and react with an entirely
unrelated surface antigen present on cells from
diferent mammalian species
HETEROPHILE ANTIBODIES
titer ratio of heterophile antibodies
Titer of 1:56
Heterophile antibodies (HAb) are made up usually of
IgM isotype
formed against Forssman antigens have the ability to agglutinate sheep RBCs
FORSSMAN ANTIBODIES
classic example of heterophile antigen, found on the surface of RBCs of many species
Glycolipid protein
Simple titration of sheep cell agglutinins
PAUL-BUNNELL-DAVIDSOHN TEST
Forssman antibodies are found in
normal serum
serum sickness
what specimen is added to dilutions of patient’s
serum
Sheep RBCs
PAUL-BUNNELL-DAVIDSOHN TEST is done with what specimen
inactivated serum
the serum must be heated at what temp
56 degrees Celsius
primary substrate of monospot test
equine erythrocytes
what does monospot test detects against ebv infection
specifc heterophile antibodies
Suspension of polystyrene latex particles coated
with ,,, from bovine red cell membranes.
highly purifed Paul-Bunnell antigen
