MONOSPOT LATEX

Question 1

Also known as “KISSING DISEASE”

Answer 1

INFECTIOUS MONONUCLEOSIS

Question 1

Causative agent of infectious mononucleosis

Answer 1

EPSTEIN-BARR VIRUS

Question 2

mode of transmission of IM

Answer 2

• intimate contact
• saliva
• blood transfusion
• semen

Question 3

diagnosis of IM

Answer 3

Hematological and serological MTDS

Question 4

what diseases can be diagnosed using the hematological method

Answer 4

lymphocytosis with
prominent atypical lymphocytes

Question 5

what diseases can be diagnosed using the serological method

Answer 5

(+) heterophile and EBV antibodies

Question 6

triad manifestation of IM that can last 1-4 weeks

Answer 6

Fever
pharyngitis,
cervical lymphadenopathy

Question 6

EBV is also a cause of

Answer 6

Burkitt’s lymphoma
Nasopharyngeal carcinoma

Question 7

Composed of a broad range of generally weak
antibodies induced by heterophile antigens

Answer 7

HETEROPHILE ANTIBODIES

Question 8

The antibodies present in IM are:

Answer 8

EBV antibodies (Specifc)
Heterophile antibodies (Non-specifc)

Question 8

A defnitive diagnosis can be established by

Answer 8

SEROLOGICAL ANTIBODY TESTING

Question 9

Stimulated by one antigen and react with an entirely
unrelated surface antigen present on cells from
diferent mammalian species

Answer 9

HETEROPHILE ANTIBODIES

Question 10

titer ratio of heterophile antibodies

Answer 10

Titer of 1:56

Question 10

Heterophile antibodies (HAb) are made up usually of

Answer 10

IgM isotype

Question 11

formed against Forssman antigens have the ability to agglutinate sheep RBCs

Answer 11

FORSSMAN ANTIBODIES

Question 11

classic example of heterophile antigen, found on the surface of RBCs of many species

Answer 11

Glycolipid protein

Question 11

Simple titration of sheep cell agglutinins

Answer 11

PAUL-BUNNELL-DAVIDSOHN TEST

Question 11

Forssman antibodies are found in

Answer 11

normal serum
serum sickness

Question 12

what specimen is added to dilutions of patient’s
serum

Answer 12

Sheep RBCs

Question 12

PAUL-BUNNELL-DAVIDSOHN TEST is done with what specimen

Answer 12

inactivated serum

Question 13

the serum must be heated at what temp

Answer 13

56 degrees Celsius

Question 14

primary substrate of monospot test

Answer 14

equine erythrocytes

Question 15

what does monospot test detects against ebv infection

Answer 15

specifc heterophile antibodies

Question 16

Suspension of polystyrene latex particles coated
with ,,, from bovine red cell membranes.

Answer 16

highly purifed Paul-Bunnell antigen

Question 17

result in heterogenous solution

Answer 17

CLUMPING

Question 18

result in homogenous solution

Answer 18

NO CLUMPING

Question 19

Monospot Latex Reagent that act as preservative

Answer 19

sodium azide 0.1%

Question 20

Positive control is reactive/non-reactive with diluted human serum

Answer 20

reactive

Question 21

negative control is reactive/non-reactive with diluted human serum

Answer 21

non-reactive

Question 22

Qualitative immunochromatographic assay

Answer 22

TEST CASSETTE

Question 23

what is the first reagent in test casette

Answer 23

Goat antihuman-IgM blue latex conjugate

Question 24

Second reagent in test casette

Answer 24

Bovine erythrocyte extract

Question 25

interpret Negative test

Answer 25

NO agglutination

Question 25

the presence of agglutination indicates CLINICALLY SIGNIFICANT CONCENTRATION of infectious mononucleosis antibody in the sample.

Answer 25

qualitative technique

Question 26

the appropriate titer will correspond to the highest dilution that still presents a clearly visible agglutination.

Answer 26

semiquantitative technique

Question 27

interpret weak-positive result

Answer 27

Slight agglutination

Question 27

interpret positive result

Answer 27

Agglutination