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MEDL 385 Histo 2 > Module 8: Silver Impregnation Techniques > Flashcards

Module 8: Silver Impregnation Techniques Flashcards

1
Q

What fiber is demonstrated by the argyrophilic reaction?

A

Type III collagen

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2
Q

What is the role of ferric ammonium sulphate (iron alum) in the argyrophilic silver reaction?

A

Sensitization

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3
Q

Which tissue would make a good control for argyrophilic silver methods?

A

Liver

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4
Q

List the main steps of the argyrophilic silver reaction

A

Oxidation, sensitization, impregnation, reduction, toning, removal of unreduced silver

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5
Q

What would be a suitable control for argentaffin silver reaction?

A

Skin, small intestine

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6
Q

At what temperature is impregnation performed in the argentaffin silver reaction?

A

56 degrees C

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7
Q

Three components that stain black by the argentaffin method

A

Melanin, argentaffin granules, some neurosecretory granules

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8
Q

Main steps of the argentaffin reaction

A

impregnation (ammoniacal silver), toning (gold chloride), removal of unreduced silver (sodium thiosulfate)

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9
Q

What must occur in order for fungi to take part in the silver reaction?

A

Carbohydrates in the cell wall must be oxidized to aldehydes

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10
Q

What prevents tissues with small amounts of polysaccharides from taking part in the silver reaction in the modified argentaffin method?

A

They are further oxidized to nonreactive end products

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11
Q

What would make a good control for the modified argentaffin reaction

A

Tissue known to contain fungi

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12
Q

Main steps of the modified argentaffin reaction

A

Oxidation, impregnation, toning, removal of unreduced silver

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13
Q

How can the detachment of tissues from slides that occurs in silver impregnation methods be overcome?

A

Use commercially prepared coated slides

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14
Q

Expected results for the argyrophilic silver method

A

Reticulin - black Nuclei - grey Collagen - taupe or light red

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15
Q

Expected results for the argentaffin method

A

Melanin and argentaffin granules - black Nuclei and background - red

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16
Q

Expected results for the modified argentaffin method

A

Fungi and Pneumocystis jiroveci - black Mucins - taupe Background - green

17
Q

What staining technique is this? What is being demonstrated?

A

Argentaffin silver

Melanin in skin

18
Q

What staining technique is this? What is being demonstrated?

A

Argyrophil silver

Reticulin (collagen III) in liver

19
Q

What staining technique is this? What is being demonstrated?

A

Modified argentaffin

Fungi (Pneumocystis) in infected lung tissue

MEDL 385 Histo 2 (12 decks)

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