Module 2: General Staining Methodology

Question 1

Over staining of tissues and removing excess stain from unwanted parts of the tissue

Answer 1

Regressive

Question 2

Gradual removal of dye from tissues using microscopic control

Answer 2

Differentiation

Question 3

Staining which requires an intermediate substance for the dye to interact with the tissue

Answer 3

Indirect

Question 4

Staining of tissues so that at the end of the correct time in the stain solution the tissue is staining the appropriate colour

Answer 4

Progressive

Question 5

Holds a dye in combination with tissue or bacteria

Answer 5

Trapping agent

Question 6

Staining when the dye interacts directly with the tissue

Answer 6

Direct

Question 7

Secondary dye that stains additional tissue elements contrasting colours from the primary dye

Answer 7

Counterstain

Question 8

Enhance staining in metallic impregnation methods for the nervous system

Answer 8

Accelerator

Question 9

Intermediate substance required by some stains so they may combine with a tissue structure

Answer 9

Mordant

Question 10

Enhance staining or increase selectivity but are not required for staining to occur

Answer 10

Accentuator

Question 11

Macroscopic removal of visible colour

Answer 11

Decolourization

Question 12

Six ways differentiation can be accomplished

Answer 12

Simple solvents, acid differentiation, mordant differentiation, action of other dyes, action of bases, action of oxidizers

Question 13

Which factors will affect tissue staining by influencing the tissue binding sites available?

Answer 13

Choice of fixative and pH

Question 14

Which pigment is termed ‘wear and tear’ pigment found in aging cells of the heart, liver, and adrenal glands?

Answer 14

Lipofuchsin

Question 15

Which pigment is found in malaria parasites and in infected organs?

Answer 15

Hemozoin