Module 10: Myelin and Nissl Staining

Question 1

Expected results from Luxol Fast Blue - Cresyl Echo Violet method

Answer 1

Normal myelin - blue

Nuclei, nissl substance - purple

Question 2

Location of myelin

Answer 2

Sheath around nerve fibres (axon)

Question 3

Location of nissl substance

Answer 3

Cytoplasm and dendrites of neuron

Question 4

Composition of myelin

Answer 4

Cholesterol, phospholipids, cerebrosides, lipoprotein

Question 5

Composition of nissl substance

Answer 5

Rough ER, RNA content

Question 6

Conditions myelin may be reduced in

Answer 6

MS, Pernicious anemia, nerve damage

Question 7

Conditions nissl substance may be reduced in

Answer 7

Neuron injury

Question 8

Is myelin stained by Luxol Fast Blue or Cresyl Echo Violet?

Answer 8

Luxol Fast Blue

Question 9

Is nissl substance stained by Luxol Fast Blue or Cresyl Echo Violet?

Answer 9

Cresyl Echo Violet

Question 10

What other stains may be used to demonstrate myelin?

Answer 10

H+E, IHC techniques

Question 11

What other stains may be used to demonstrate nissl substance?

Answer 11

H+E, PAS

Question 12

What kind of dye is luxol fast blue?

Answer 12

Copper phthalocyanin

Question 13

Luxol fast blue staining is due to the presence of what?

Answer 13

Lipoprotein