Module 6: Amyloid Staining

Question 1

What is the composition of an amyloid?

Answer 1

Mainly protein with a small carbohydrate component

Question 2

What colour of birefringence is coupled with Congo red to demonstrate amyloid?

Answer 2

Apple green

Question 3

Term that describes the ability of an amyloid to polarize light

Answer 3

Dichroic

Question 4

2 staining principles in Congo red

Answer 4

Van der Waal’s and hydrogen bonding

Question 5

How thick should sections for amyloid be cut?

Answer 5

8-10 micrometers

Question 6

How is Congo red classified?

Answer 6

Acidic diazo dye

Question 7

Expected staining results of Congo red under a light microscope:

Answer 7

Amyloid - pink to red

Nuclei - blue

Question 8

Expected staining results of Congo red under a polarizing microscope:

Answer 8

Amyloid - apple green

Question 9

When staining with Congo red, hydroxyl radicals in tissue are formed by pre treatment with what?

Answer 9

Alkali salt solution

Question 10

Amyloid filament can be described as what at the molecular level?

Answer 10

Beta pleated sheet

Question 11

What type of molecular structure does Congo red dye have?

Answer 11

Linear