Module 4: Lipid Staining Flashcards
1
Q
What is the principle of the Oil Red O stain?
A
Preferential solubility/selective solubility
2
Q
Which fixative can be used to demonstrate lipids by blackening unsaturated bonds?
A
Osmium tetroxide
3
Q
Which mounting media would be acceptable to use for cover slipping sections stained using Oil Red O?
A
Glycerin jelly
4
Q
List 4 criteria for preferentially soluble dyes
A
Highly coloured
More soluble in fat than in its solvent and not water soluble
Treatment with dye must not remove the fat
Solvent must not remove the fat
5
Q
List 4 commonly used preferentially soluble dyes
A
Oil Red O, Sudan III, Sudan IV, Sudan Black B
6
Q
Expected results after Oil Red O
A
Lipids - red
Nuclei - blue
Other tissue - light blue-grey
