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MEDL 385 Histo 2 > Module 12: Immunohistochemical Staining > Flashcards

Module 12: Immunohistochemical Staining Flashcards

1
Q

3 IHC labelling methods:

A

Enzyme/chromogen, colloidal gold, fluorochromes

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2
Q

What is the most common type of label used in IHC?

A

Enzyme/chromogen

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3
Q

Why is tissue preservation and processing so important in IHC?

A

Poor preservation and processing may result in antigen destruction

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4
Q

What is the most common fixative used prior to IHC staining?

A

Formalin based

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5
Q

What is the pretreatment of tissue to unmask antigens termed?

A

Antigen retrieval

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6
Q

What is the process used to prevent endogenous enzymes, such as peroxidase, from taking part in the enzyme/chromogen reaction in IHC techniques called?

A

Blocking

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7
Q

List 2 common enzymes used in IHC staining

A

Horseradish peroxidase, calf intestine alkaline phosphatase

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8
Q

The commonly used chromogen DAB gives what what colour end product?

A

Brown

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9
Q

The commonly used chromogen AEC gives what what colour end product?

A

Rose-red

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10
Q

Is the direct method more or less sensitive than the indirect method?

A

Less sensitive

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11
Q

Avidin used the ABC (avidin-biotin conjugate) method is obtained from what source?

A

Egg white

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12
Q

What is the benefit of using Streptavidin in the LSAB (labeled streptavidin-biotin) method compared to avidin in the ABC method?

A

Streptavidin results in less nonspecific staining

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13
Q

True or false: Biotin blocking is not required in polymer detection

A

True

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14
Q

True or false: Resinous mounting media should be used if the chromogen is AEC

A

False

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15
Q

True or false: Harris hematoxylin is the counterstain of choice for all methods using alkaline phosphates as a chromogen

A

False

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16
Q

True or false: Serum blocking may be performed to prevent nonspecific binding of the primary antibody

A

True

17
Q

True or false: A positive control only needs to be run when a new batch of primary antibody is received

A

False

18
Q

True or false: The sensitivity of polymeric detection is similar to LSAB

A

True

MEDL 385 Histo 2 (12 decks)

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