Module 3: Routine Staining (H&E) Flashcards
What stain and step is this?
What happened in the tissue?
H+E after hematoxylin step
Everything stains blue
What stain and step is this?
What happened in the tissue?
H+E after acid alcohol (differentiation)
Nuclei stain red, cytoplasm colourless
What stain and step is this?
What happened in the tissue?
H+E after blueing
Nuclei blue, cytoplasm colourless
Stain becomes insoluble (permanent)
What stain and step is this?
What happened in the tissue?
H+E final
Nuclei blue, cytoplasm and other tissue elements different shades of pink
What two processes must hematoxylin go through in order to become a dye?
Oxidation and mordanting
What reagent do we use for the nuclear staining step in the H+E procedure?
Harris hematoxylin
What is hematoxylin oxidized to?
Hematein
Two processes in which hematoxylin can be oxidized
Spontaneous atmospheric oxidation
Use of chemical agents
What are the two main classifications of lakes?
Alum/aluminum, ferric/iron
Which classification of lake is very unstable?
Ferric
Name one type of hematoxylin that uses an unstable lake
Weigert’s
What are the two terms used to describe the principle of the oil red O procedure?
Selective solubility, preferential solubility
List 5 chemical agents which may be used to convert hematoxylin to hematein
Sodium iodate, mercuric chloride, hydrogen peroxide, potassium permanganate, ferric chloride
Which stain is an alternative to hematoxylin sometimes used by some for nuclear staining
Celestine blue
True or false: Harris hematoxylin is suitable for frozen sections
True
