Module 2: Tools & Techniques of Micro Lab

Question 1

Name the four methods (Overview) that can be used to recover and identify microorganisms?

Answer 1

• Culture and Biochemical Test
• Microscopy
• Immunodiagnostic Test
• Detection of Microbial Genes

Question 2

What does the type of culture and testing depend on when using Culture and biochemical tests for recovery and ID of microorganisms?

Answer 2

Anticipated pathogen and testing conditions (e.g. time, reagents, instruments)

Question 3

This Culture and biochemical test is rapid, sensitive but possibly less specific, and less expensive?

Answer 3

Screening/Presumptive

Question 4

This Culture and biochemical test is less rapid, more specific, and more expensive?

Answer 4

Definitive/Confirmatory

Question 5

Name the major types of Bacterial culture media.

Answer 5

• Nutrient (General)
• Enriched
• Selective
• Differential

Question 6

This bacterial culture media provides general nutrients required for growth of most common bacteria?

Answer 6

Nutrient (General) bacterial culture media

Question 7

This bacterial culture media contains ingredients that restrict the growth of certain types of bacteria (e.g. antibiotics, inhibitory chemicals, etc…)?

Answer 7

Selective bacterial culture media

Question 8

This bacterial culture media provides general nutrients plus various enrichments required for growth of common and more fastidious bacteria?

Answer 8

Enriched bacterial culture media

Question 9

This bacterial culture media contains ingredients that visually indicate certain chemical reactions caused by bacterial growth (e.g. detects pH change, H2S production, metabolism of specific chemicals)?

Answer 9

Differential bacterial culture media

Question 10

Blood Agar Plate (BAP) uses what as the culture medium?

Answer 10

Sheep Blood

Question 11

This Agar Plate has a moderately enriched medium, grows almost all bacteria, permits visual observation of blood cell hemolysis, and can be made to be selective with the addition of antibiotics?

Answer 11

Blood Agar Plate (BAP)

Question 12

Which Agar Plate is unable to grow Neisseria gonorrhoeae & Haemophilus influenza?

Answer 12

Blood Agar Plate (BAP)

Question 13

Which Agar has a highly enriched media, grows most medically significant bacteria, and may contain antibiotics?

Answer 13

Supplemented Chocolate Agar (SCA)

Question 14

Which Agar is required to grow Neisseria Gonorrhoeae and Haemohpilus Influenzae?

Answer 14

SCA (Supplemented Chocolate Agar)

Question 15

Name the highly enriched-selective media (SCA) with antibiotics to inhibit most bacteria while allowing pathogenic Neisseria species to grow?

Answer 15

Thayer-Martin (TM) or Lewis-Martin Medium

Question 16

Which Agar is selective for gram-negative bacilli?

Answer 16

MacConkey Agar (MAC)

Question 17

This Agar contains inhibitors for gram-positive cocci/bacilli and gram-negative cocci?

Answer 17

MacConkey Agar (MAC)