Module 2 - Biology Flashcards
DNA sequence in genes code for polypeptide molecules such as pepsin and titin.
Explain why a process known as transcription is necessary for polypeptide synthesis.
Transcription is necessary for polypeptide synthesis because the gene is copied to the mRNA. And the RNA goes to the ribosomes for polypeptide synthesis as the DNA is too large to fit through the nuclear pores.
Titin is a fibrous protein. Pepsin is a globular protein. Compare the properties and functions of fibrous proteins and globular proteins in the human body.
Fibrous protein are insoluble, elongated strands, strong and flexible. They are used for things like protection, e.g. keratin in skin, hair and nails.
Globular protein is soluble, spherical in shape and a tertiary structure.
It is used to package DNA, and transport substances in blood. e.g haemoglobin in RBC carry oxygen.
State the role of a gene
It codes for one or more polypeptide
Explain how the structure of DNA allows replication
DNA is double stranded and both strands act as templates.
The hydrogen bonds between the complementary base pairings can easily be broken allowing replication to occur.
However the hydrogen bonding between A&T and C&G are different.
There are 3 H bonds in C&G and 2 H bonds between A&T.
Explain why phenolphthalein indicator was able to detect the breakdown of triglycerides.
When triglycerides breakdown they form fatty acids. More breakdown means the pH falls which the indicator can detect.
What is a triglyceride
Triglyceride is made up of a glycerol and 3 fatty acids.
It usually has a high ratio of Hydrogen to Oxygen
Explain how the structure of phospholipids allow them to form the bilayer of a plasma membrane
Phospholipids have a hydrophobic tail and hydrophilic heads. The hydrophilic heads form H bonds with the water and the hydrophobic tails face towards each other.
What is the structure of an amino acid.
Central Carbon attached to Carboxyl group (COOH) , Amino group (NH2) and side chain (R)
Orlistat is a drug that directly reduces the breakdown of fat in the small intestine. This reduction in breakdown leads to less fat being absorbed into the body.
Suggest the mechanism by which orlistat reduces the breakdown of fat.
Enzyme Inhibitor
Define the term balanced diet
Balance diet means consuming nutrients in the correct proportions
Suggest and explain why over consumption of milk and beef can lead to malnutrition.
Milk and beef are both triglycerides and both contain large amounts of energy, more than what a human needs.
Over consumption increases fat storage which in turn leads to obesity and other health conditions.
Eating lots of dairy and meat could also mean the diet is not balanced anymore.
What are purines and pyrimidines.
Purines have 2 carbon rings - Adenine and Guanine
Pyrimidines have a carbon ring - Cytosine and Thymine
DNA carries the genetic code which is non overlapping and degenerate.
What does non overlapping and degenerate code mean?
Each nucleotide is only part of one triplet of bases and more than one triplet codes for a amino acid.
What is the correct definition of the term coenzyme?
A non protein organic molecule, not permanently attached to an enzyme but needed to allow the enzyme to function
Explain why onion root tips are used to view cells undergoing mitosis.
It is where the cells are able to divide , as it is a meristem, and no chloroplasts are present.
Suggest a stain that a student can use to highlight the nuclei of cells during mitosis
Methylene blue
Carl Woese suggested a new top level taxon - domain.
He used his results from studying RNA
Name the cell component that appears in organisms of all 3 domains that Woese suggested.
Ribosomes
One of the domains is called Eukarya.
Name the other 2 domains
Bacteria
Archaea
State 2 defining features of all members of the domain Eukarya
Nucleus
Linear DNA
80s ribosomes
Which stage in the cell cycle is DNA polymerase most active
S phase
Describe the function of DNA polymerase
Make phosphodiester bonds between adjacent nucleotides.
What is the oder of increasing complexity of organisation within an organism?
Cell –> Tissue –> Organ –> Organ System
What elements are found in proteins?
Carbon, Hydrogen, Oxygen. Nitrogen, Sulphur
Human pancreatic lipase breaks the bonds between fatty acids and glycerol.
What name is given to this reaction>
Hydrolysis
A zygote undergoes rapid cell division.
Explain why the type of nuclear division in a zygote is mitosis and not meiosis.
Mitosis is used for the growth of a zygote where it needs to have identical genetic information.
Explain the role of embryonic stem cells in the development of the embryo.
Embryonic stem cells are undifferentiated cells, they can differentiate into anything and become specialised in its particular function - developing the foetus.
A blastula is an animal embryo at an early stage of development. As the blastula develops, it becomes a hollow ball of cells with an inner cell mass; which is a source of embryonic stem cells.
Explain why the cells of the inner cell mass are not totipotent stem cells.
They are not totipotent because they cannot form whole organisms. They cannot form some tissues such as the placenta
Blastula are pluripotent
Collagen molecule has 3 polypeptide chain each with 1050 amino acids, wrapped into a triple helix.
Describe and explain why collagen is a fibrous protein.
Collagen is a fibrous protein because it is a long chain of amino acids.. It is strong and flexible and it is insoluble. It has a structural function to provide strength.
Suggest why collagen is such a strong molecule
There are many hydrogen bonds between the polypeptides
There are crosslinks between collagen molecules
What happens in a RER
Translation of some proteins takes place in a eukaryotic cell
Describe the structure of the rough endoplasmic reticulum
It has a phospholipid bilayer and there are a network of membranes enclosing a fluid filled space. The surface is covered with ribosomes.
Explain the role of the membrane in the RER
Compartmentalisation and separates synthesised proteins from the cell cytoplasm
Explain how the nucleotides in a DNA molecules are arranged as two polynucleotide strands.
DNA molecules are made up of nucleotides. These are held together by phosphodiester bonds.
There are hydrogen bonds between the complementary bases. 2 hydrogen bonds between A and T and 3 hydrogen bonds between C and G.
Two antiparallel (running in opposite directions) polynucleotide strands twist to form the DNA double-helix.
Name 2 enzymes involved in DNA replication
helicase - separating
DNA ligase - joining
Explain why enzymes are essential to all organisms.
Enzymes are biological catalyst which speed up the reaction and lowers the activation energy so reactions can take place at low temperatures.
what is a protoctist
An eukaryotic organism
It has a nucleus but no cell wall
State 3 changes that the student would need to ensure to do / improve when doing a biological drawing.
The label lines should not cross
There should be no arrowheads
No shading or colouring
Both a TEM and a SEM can be used to view the same cell. However, the images formed will be different.
Compare the resolutions of these microscopes and the images formed by them. [4]
TEM has a higher resolution between 0.05 and 2nm. It shows the cells organelles such as the nucleus and the mitochondria.
SEM has a lower resolution between 5 - 50nm and it shows the surface of the cell rather than the organelles. However it does provide a 3D image.
Where are erythrocytes and neutrophils formed?
In the bone marrow
What is one of the places ciliated epithelial cells are found in?
Trachea
The cell wall of the guard cell is thicker or thinner on the side furthest away from the stoma?
Thinner
Erythrocytes and neutrophils are both examples of specialised blood cells.
Squamous and ciliated epithelial cells are also examples of specialised cells.
Describe how each of these four cells is specialised for its function. [6]
Erythrocytes are red blood cells. They are specialised because they have no nucleus and have biconcave discs to increase the surface area to volume ratio. This means they can carry more oxygen to transport around the body. In addition they are flexible, meaning they can fit through narrow gaps of the capillary tubes.
Neutrophils are white blood cells. They have a multilobed nucleus making it easier for them to squeeze through small gaps to get to the site of infections. The granular cytoplasm contains main lysosomes that contain enzymes used to attack pathogens.
Squamous epithelial cells are very thin and one cell thick, therefore rapid diffusion can occur and exchange of gases at the alveoli
Ciliated epithelial cells have cilia which move in rhythm to move mucus and trapped pathogens.
Suggest why goblet cells have large number of mitochondria.
They provide a lot of energy
Suggest how the role of the Golgi apparatus is relevant to the function of the goblet cell.
The Golgi apparatus packages protein and aids secreting the mucus in exocytosis
Sjogren’s syndrome is a rare condition that can reduce the production of mucus.
Suggest how the upper respiratory tract of a place with Sjogren’s syndrome might be affected.
More infections leading to coughing
How are two amino acids joined together
Peptide bonds
The process of esterification leads to the formation of ester bonds during the production of triglycerides.
Describe what is meant by the term esterification
The reaction between carboxylic acid and alcohol. It is a condensation reaction and removed H20
Explain the meaning of the phrase semi conservative replication
Semi conservative replication is when each strand of DNA acts as a template strand for a new double helix
DNA ligase is one enzyme involved in the replication of DNA
State 2 other enzymes involved and describe their functions
DNA helicase unzips the DNA molecules by breaking the hydrogen bonds and separating the 2 strands
DNA polymerase forms phosphodiester bonds forming the sugar phosphate backbone
Give one way that an image produced by a laser scanning confocal microscope differs from that produced by an electron microscope
Laser scanning confocal has a Lower resolution compared to electron microscope
It also allows live species to be viewed
Uses fluorescent tags
The use of stem cells is being evaluated for the treatment of certain human diseases
Name 2 potential sources of human stem cells and for one source, describe an ethical issue associated with the use of stem cells.
Embryonic and bone marrow. The ethical issue with use embryonic sources is that embryos die, some may believe that lives are being killed
What observations are made at prophase
In prophase chromosomes are condensed
Nucleolus is not visible