Module 11: Testing and Laboratory Procedures Flashcards
Urine testing - random urine
Patient urinates in a clean, nonsterile container. This specimen is used for screening purposes
Urine testing - First morning specimen
The patient collects the first specimen of the morning in a clean container. This specimen is more concentrated and used for pregnancy testing, or when other analytes (protein, nitrites) need to be evaluated
Urine testing - Clean-catch midstream
The patient performs perineal cleaning using moist wipes, begins to urinate, and then collects the specimen midstream in a sterile urine container. This specimen is used for cultures or when a noncontaminated specimen is desired
Urine testing - 24-hour
This method uses a container with preservatives. The patient discards the first morning specimen and collects all specimens for the next 24 hr, including the first void of the second day.
Important in quantitative analysis of components such as protein when analyzing kidney function
Urine testing - Catheterized collection
This method is used when a sterile urine sample is needed or if patients are unable to provide a specimen on their own. It involves insertion of a sterile tube through the urethra into the bladder. This procedure is performed by the provider or nurse, with the medical assistant prepping the patient and assisting.
Fecal occult blood test
Stool specimen screened for the presence of blood. Patient will avoid specific medications and foods to avoid for 3 days. Three separate specimens on filter paper.
Control strip turns blue
blue is positive for blood
Tape test
Parents are given a slide with a piece of tape on it. The tape is placed over the child’s anus before getting up in the morning and then back on the slide, which is brought in for analysis
Stool specimens infections
Specific containers are used for each of these tests. O&P testing detects the presence of parasites and their eggs, either of which require treatment
Sputum specimens
A deep, productive cough is necessary to produce a specimen from the lungs. Best collected in early morning
Precautions and requirements for specimen collections
Take precautions to avoid touching the insides of lids, swabs, or containers
Collect the specimen at right time, from site of suspected infection.
Minimize transport time to a reference lab, collect the appropriate quantity.
Use the appropriate containers and label them accordingly
What does CLIA-waived testing mean?
Simplest of laboratory procedures. Medical assistants may perform these tests per provider request
Point of care testing: Pregnancy testing
Urine is screened for the presence of human chorionic gonadotropin (hCG) antibodies
Point of care testing: Rapid strep
Throat swabs are obtained to screen for Group A streptococcus
Point of care testing: dipstick, tablet, or multi-stick urinalysis
Urinalysis is a screening tool for analytes that are excreted in the urine
Point of care testing: hemoglobin
A machine is used to screen for the oxygen-carrying protein in whole blood, performed using capillary blood from a fingerstick (capillary puncture)
Point of care testing: Spun hematocrit
Fingerstick collection of blood in microcapillary tubes is centrifuged and evaluated for the percentage of red blood cells
Point of care testing: blood glucose
Whole blood is analyzed in a glucometer for a quantitative glucose level and is a screening test for diabetes, performed using capillary blood from a finger stick (capillary puncture)
Point of care testing: Hemoglobin A1c
Capillary blood test shows diabetes control over an approximate 3 month period
Point of care testing: Cholesterol testing
Lipids are evaluated using capillary blood
Point of care testing: Helicobacter pylori
A blood sample screens for H pylori, the main cause of gastric ulcers
Point of care testing: Mononucleosis screening
Tests for the presence of the Epstein-Barr virus in capillary blood
Point of care testing: nasal smear for influenza types A and B
Qualitative test for influenza antigens using a swab that is inserted into the nostril
Point of care testing: drug testing
Substances can be detected in urine and blood samples
Point of care testing: fecal occult blood
Test performed to screen for hidden blood in the stool
CLIA regulations: moderate- and high-complexity
Nonwaived, labs must have a CLIA certificate and undergo inspections to ensure standards are being met
CLIA regulations: provider-performed microscopy procedures
Considered a form of moderate-complexity testing, but CLIA approved to allow the provider to develop a preliminary diagnosis and begin treatment as warranted
COLA
Commission on Office Laboratory Accreditation, an independent accreditor for laboratories, focuses on meeting CLIA regulations
Quality assurance vs quality control
Quality assurance is comprehensive, relates to policies and procedures that must be implemented for reliability of test results
Quality control is a more specific part of quality assurance, it attempts to uncover errors and eliminate them.
Ex:
Reviewing expiration date, documenting temperature of laboratory refrigerator is quality control
Rotating stock to put newest containers in back of storage, maintaining proper temperature of laboratory refrigerator is quality assurance.
Quality control logs
Responsibility of medical assistant to monitor function of equipment within facility. Logbooks need to be maintained to validate the test was conducted under optimal conditions, control samples were accurate prior to testing patient samples.