Module 1 poll and assignment questions Flashcards
What is the best definition of microbiology?
a. The study of unicellular organisms
b. The study of things that are not visible to the naked eye
c. The study of bacteria and viruses
d. The field of study that uses microscopes
B.
Which of the following is NOT influenced by microbes?
a. water quality
b. production of milk
c. production of fermented food
d. production of insulin
e. none of the above
f. all of the above
e. none of the above
The actions of microbes were known prior to the invention of the microscope. Which is an example of ancestral knowledge of microbiology?
a. use of milk fermentation to produce yougurt and cheese
b. the discovery of viruses
c. the discovery of penecillin
d. the use of escherichia coli to produce insulin
A
Which of the following is not a microbe?
a. SARS-CoV-2
b. E. coli
c. water bears
d. midichlorians
e. blue/green mold on your bread
f. none of the above
F
Why are viruses absent from the tree of life?
a. viruses are not alive
b. viruses are not metabolically active
c. viruses are from space
d. viruses are not cellular microbes and lack rRNA
D
which of the following would not be considered microbiology?
a. discovery of food fermentation
b. the use of latrines by romans
c. bad smell/air cause disease
d. use of medical plants
C
When thinking about microscopy, which parameter(s) are important?
a. contrast
b. resolution
c. magnification
d. All of the above
e. None of the above
D.
If ou have a 10x objective lens and a 40x objective lens, what is the total magnification?
a. 4000x
b. 40x
c. 50x
d. 400x
d.
What would be the outcome if you use a basic dye (blue) and add dye (pink) to stain a bacterial sample?
a. Both mix and create a brownish stain
b. Each would bind to a specific structure and help differentiate
c. cancel each other out
d. bind to the same structure
b.
You skipped the alcohol step while doing a gram stain. What the color of the gram-negative bacteria?
a. pink
b. purple
c. magenta
d. dark red
e. brown
b.
You replace carbol fuchsin (pink) with a green dye called malachite green. What would be the colour of the acid-fast bacteria?
a. green
b. violet
c. brown
d. yellow
a.
How would you improve resolution and contrast (without staining) of light microscopy?
a. increase magnification
b. increase illumination
c. change the property of light source
d. deacrease illumination
c.
You have discovered a new type of bacteria and you suspect it forms endospores. What type of microscopy can be used to observe sporulation in real time?
a. brightfield with spore stain
b. phase contrast without stain
c. darkfield without stain
d. brightfield without stain
b.
How would you improve maginification and resolution of your microscope?
a. decrease wavelength of your illumination source
b. increase wavelength of your illumination source
c. better lenses
d. have bionic eyes
a.
Select all the statements that explains why microbiology is important and relevant to your everyday life
a. microbes are used to create fermented food such as yogurt
b. microbes provide nitrogen to plants used for agriculture
c. microbes found in the intestinal tract of mammals provide vitamins and nutrients
d. microbes cause disease
all of the above
Please select the correct statement about the relationship between bacteria, archaea and eukarya
a. Archaea are ancient Bacteria and Bacteria are ancient Eukarya
b. Eukarya arose from a group of Archaea called Asgard and Eukarya later evolved endosymbiotic relationship with Bacteria
c. Evolutionary speaking, Archaea and Bacteria are more closely related to each than to Archaea and Eukarya
d. Eukarya arose independently of bacteria and archaea
B
Which of the following statement about the miasma theory is correct?
Miasma theory states that diseases are caused by microbes
Miasma theory states that diseases are caused by bad air
Miasma theory is the current scientific explanation for the origin of diseases
Miasma theory was developed by Louis Pasteur
B
Match the following
Group A:
1. 3D-like image of a surface
2. High resolution image with sub-cellular structure
3. Bright object on a dark background
4. “white glow” objects on dark background
Group B:
1. TEM
2. Phase contract
3. SEM
4. Darkfield
1-3
2-1
3-4
4-2
Please select the correctly formatted bacterial name
Escherichia coli (italicized)
Escherichia Coli (italicized)
Escherichia coli
escherichia coli (italicized)
A
What are the correct steps in order of a gram stain?
-Fix sample with heat
-Add crystal violet (purple dye)
-Add the iodine mordant
-Decolorize with alcohol
-Add Safranin (pink dye)
What is microbiology?
The study of microbes, which includes both cellular and acellular entities, often invisible to the naked eye, such as bacteria, archaea, fungi, protists, viruses, and prions
The study of chemical reactions and properties of substances
The study of human anatomy and physiology
The study of large organisms visible to the naked eye, such as plants and animals
A
Please select the best statement about the importance of staining
Staining is only used by medical microbiologist
Staining improves contrast, helps categorize bacteria, helps identify structures and is an invaluable tool for medical microbiologist
Staining only improves contrast
Staining is a tedious task to make undergraduate student life difficult
B
Match the scientists and their contribution to the gold age of microbiology
Group A:
1. Sergei Winogradsky
2. Louis Pasteur
3. Antoine van Leeuwenhoek
4. Robert Koch
Group B:
1. Pasteurization
2. Did not contribute
3. Established criteria for casual relationship between microbes and diseases
4. Cross-feeding between microbes
