MOD 6 BIOSAFETY Flashcards
intentional release of pathogenic microorganisms aims to protect public health and the environment
biosafety
containment controls of biosafety level (principles of laboratory)
Laboratory practices
- Safety equipment
- Facility construction
barrier that can use as a personal protective equipment between lab worker and pathogen
physical barriers (mga sinusuot)
type of barrier that has structural aspects of the laboratory that make working environment safer against
infection
Secondary barriers
replace the hazard
substitution
change how people works like social distancing
adminstrative controls
refers to the equipment and facility design reduce exposer to the hazard eg; biosafety cabinets
engineering controls
physically remove hazard (vaccines)
HIERARCHY OF CONTROLS
PPE
Administrative controls
engineering controls
substitution
elimination
BSL that has an agents not known to cause disease in healthy adults
Biosafety Level 1
agents in Biosafety Level 1
Bacillus subtilis, Naegleria
gruberi, infectious canine hepatitis virus,
non-pathogenic E. coli species
Biosafety cabinets that for operation protection (for person only)
class 1 Biosafety cabinet
Biosafety cabinets that for protection of spealized laboratory dealing with deadly pathogen
class 3
Primary hazards of biosafety level 2
- accidental needle sticks
- exposure to eyes and nose (mucous membranes)
- ingestion of infectious material
Biosafety cabinets that for operational and environment is protected also the product
class 2
Agents associated with human disease generally required for any human-derived blood, bodily fluids, tissues in which infectious agent may be unknown
Biosafety Level 2
example agent of a biosafety level 2
measles virus, Salmonella
species, pathogenic Toxoplasma, Clostridium botulinum, hepatitis B virus
primary hazards of biosafety level 3
needle sticks & ingestion exposure to infectious aerosols
why does the west nile accur
laboratory
worker cut finger while dissecting
bird; 4 days later, had symptoms of fever, myalgia, recurring sweats, hot flashes
where does the west nile virus come from
birds
common source of
laboratory-acquired infection also known as a classified as potential biological weapon
tularemia
biosafety level with an agents that is potential for respiratory
transmission, may cause serious and potentially lethal infection
biosafety level 3
practices of biosafety level 2
policies to restrict access to lab;
- biohazard warning signs posted
outside lab;
- surveillance of laboratory personnel
with appropriate immunizations
offered;
- biosafety manual with definitions of
needed waste decontamination or
medical surveillance policies;
- supervisory staff who have experience
working with infectious agents and
specific training for laboratory
personnel in handling these agents
specific training for lab personnel in handling potentially lethal agents;
biosafety level 3
