mm

Question 1

Type of cell use in bio-pharmaceutical production for industry

Answer 1

e.coli- 24%
Yeast 13%
4% insect cell lines
56% mammalian cell lines
3%transgenic plants and animals

Question 2

Global therapeutic protein market

Answer 2

USA 40% bc of lack of regulation
EU- 25%
China 7%
all other 28%

Question 3

objectives of protein expressions in industrial production

Answer 3

• safe and contollable fermentation
• cheap fermentation
• high level of recombinant production (g/L not mg/L )
• Eady recovery of expressed proteins
• no degradation of expressed protein

Question 4

Microbial expression hosts 
E.coli 
Advantages 
and 
Disadvantages

Answer 4

numerous specific vectors ( Y phage and pUC plasmid derivatives )
- v high transformation efficiencies
- v well understood fermentations 
- simple cell recovery and lysis 
DISADVANTAGES
- not naturally competent 
-low expression yields
- no glycolysation of r protein 
- rProtein not exported