MLS PRACTICAL 1 Flashcards
What’s the most common type of microscope used?
light/optic microscope
What are light microscopes?
are microscopes that uses light to visualize a specimen/something
the objective lenses can magnify the images how many times?
4x - 100x
the ocular lens can magnify the image how many times?
10x - 15x
Steps of light passing
1- light passes through a lens from below that focuses the light on the specimens
2- light passes through the objective lenses that magnify the image 4x-10x
3-the light passes through the tube and ocular lenses that further magnify the image for 10-15 times
True or False: the maximum number of times magnified by all microscopes is 1000x.
False; 1200x
Name the components of the microscope
- eyepiece (ocular lenses)
- tube holding the eyepiece
- nosepiece
- objective lenses
- stage
- stage clips
- diaphragm
- light source
- light intensify control
- light switch
- stage adjustment (control) knob
- focus knobs (coarse and fine adjustments)
The steps of using a microscope (1-5)
1- plug in the light microscope and turn it on
2- place the specimen on the stage and hold it with the stage clips
3- move the objective lenses to the smaller magnification
4- use the coarse adjustment to clear up the image
5- use the slide control (adjustment) knob to center the specimen.
True or False: the image in the microscope is inverted so if you moved the slide image to the right, it will move to the left and vice versa.
True
The steps of using a microscope (6-10)
6- use the fine adjustment knob to refine the image. You may need to adjust the eyepiece to focus the image.
7- rotate the nosepiece to the objective lens appropriate for the purpose
8- you may need to adjust the fine adjustment but avoid using the coarse knob
9- Note what objective lens you are using when you make your observation (keep in mind that it might be 10x magnified more)
10- turn off the light, dispose of/save the specimen, clean the lenses, and replace the microscope cover
When are you supposed to use an oil immersion?
When you use the 100x objective lens
Why/When are light microscopes commonly used?
To study bacteria, slices of tissues, liquid samples, and some mitochondria
When is an inverted light microscope used?
when studying tissue culture (cells covered by liquid media)
Why are light microscopes commonly used in high schools and undergraduate labs?
- easy to use
-relatively inexpensive - can be used to visualize living specimens over time with a low likelihood of damaging the specimens
Why are light microscopes commonly used in high schools and undergraduate labs?
- easy to use
-relatively inexpensive - can be used to visualize living specimens over time with a low likelihood of damaging the specimens
State some limitations of light microscopes.
- limited in resolution and magnification
kind of to see a lot of the structures in details
