Mixed 3 Flashcards
How many cytochrome c molecules are involved in the reduction of one O2 molecule?
A. 2
B. 8
C. 4
D. 3
C. 4
Which complex in the electron transport chain accepts electrons from FADH2?
A. Complex I
B. Complex II
C. Complex III
D. Complex IV
B. Complex II
How many moles of O2 are reduced by one mole of NADH?
A. 2
B. 0.5
C. 0.25
D. 1
B. 0.5
The redox reaction of NADH and O2 forming NAD+ and H2O in the electron transport chain take place primarily in which area in the mitochondrion?
A. Outer mitochondrial membrane
B. Cristae
C. Matrix
D. Inner mitochondrial membrane
C. Matrix
Which of the following does not explain the highly negative phosphorylation potential?
A. Charge repulsion
B. Solvation effects
C. Entropy
D. Resonance
C. Entropy
What is the charge of ATP inside the cell?
A. -4
B. -1
C. 0
D. -3
E. -2
A. -4
True or False: All substrate-level phosphorylation reactions are coupled reactions.
True
Which of the following statements is FALSE about phosphorylation reactions?
A. Substrate-level phosphorylations take place in the cytosol while oxidative phosphorylations occur in the outer mitochondrial membrane.
B. Oxidative phosphorylations take more time than substrate-level phosphorylation.
C. Oxidative phosphorylations harvest more energy from a specific source than substrate-level phosphorylation.
D. Substrate-level phosphorylations require a phosphorylated compound while oxidative phosphorylations require electron transporters.
A. Substrate-level phosphorylations take place in the cytosol while oxidative phosphorylations occur in the outer mitochondrial membrane.
Which of the following electron transfer DOES NOT occur in the electron transport chain?
A. Transfer of hydrogen atom (H+ + e—)
B. Transfer of hydride (H—)
C. Transfer of superoxide radical (O2—)
D. Direct transfer of electron/s
C. Transfer of superoxide radical (O2—)
On electron acceptors in the electron transport chain, which of the following statements is TRUE?
A. NAD accepts two electrons and FAD accepts one electron in their complete reduction reactions.
B. NAD accepts one electron and FAD accepts two electrons in their complete reduction reactions.
C. Both NAD and FAD accepts two electrons in their complete reduction reactions.
D. Both NAD and FAD accepts one electron in their complete reduction reactions.
C. Both NAD and FAD accepts two electrons in their complete reduction reactions.
Consider the following sentences.
A. NAD+ absorbs at both 260 nm and 340 nm.
B. NAD+ absorbs at 340 nm but not at 260 nm.
C. NADH absorbs at both 260 nm and 340 nm.
D. NADH absorbs at 260 nm but not at 340 nm.
Which of the above sentences are true?
A. A and C
B. C only
C. A only
D. B and D
B. C only
Which of the following does not explain the highly exergonic hydrolysis of ATP in the biochemical standard state?
A. The phospholinkage in ATP is unstable inside the cell.
B. The products are well solvated.
C. There is resonance in at least one of the products.
D. The process relieves charge repulsion in the ATP molecule.
A. The phospholinkage in ATP is unstable inside the cell.
Even though the linkage is unstable primarily because of charge repulsion, this is kept stable by mechanisms inside the cell, e.g. interaction with Mg2+ ions.
True or False: The hydrolysis of ATP is more exergonic inside the cell than if it were to take place in the biochemical standard state.
True. The cell ensures that the concentration of the reactants are greater than the concentration of the products thereby giving a more exergonic process.
In the neurons, electrons from NADH enters the electron transport chain via ___ ?
A. Complex III
B. Complex IV
C. Complex I
D. Complex II
A. Complex III
The electrons bypass Complex II and are passed to mitochondrial glycerol-3-phosphate dehydrogenase via FAD which reduce ubiquinone.
An organism has 9 c subunits in the c ring of its Fo in the ATP synthase. What is the proton cost per ATP for this organism?
A. 3.7
B. 4.0
C. 2.5
D. 4.3
B. 4.0
One full rotation of the ATP synthase pumps 9 protons back to the matrix producing 3 ATP molecules at the same time. For the transport of ATP and the substrates ADP and Pi, 1 ATP molecule is needed. Add 9 + 3 = 12. 12 protons/3 ATP gives a value of 4.0
An organism has 9 c subunits in the c ring of its Fo in the ATP synthase. What is the P/O ratio for this organism?
A. 4.0
B. 3.0
C. 2.5
D. 2.7
C. 2.5
Using the consensus that 1 molecule of NADH leads to pumping of 10 protons to the intermembrane space and the previously calculated proton cost per ATP of 4.0:
10 (protons/NADH) x (ATP/4 protons) = 2.5 ATP/NADH
An organism has 9 c subunits in the c ring of its Fo in the ATP synthase. How many ATP molecules are stoichiometrically produced from 1 molecule of FADH2 in its mitochondria?
A. 2.0
B. 1.5
C. 3.0
D. 2.5
B. 1.5
Using the fact that the oxidation of 1 FADH2 in the electron transport chain starts at Complex III, 6 protons are pumped to the intermembrane space. With the computed proton cost per ATP of 4.0:
6 (protons/FADH2) x (ATP/4 protons) = 1.5 ATP/FADH2
An organism has 9 c subunits in the c ring of its Fo in the ATP synthase. Furthermore, the organism was exposed to a compound that inhibits its Complex III. In this case, how many ATP molecules are stoichiometrically produced from 1 molecule of NADH in its mitochondria assuming that oxygen is still being reduced in the electron transport chain?
A. 2.5
B. 1.5
C. 1.0
D. 3.0
C. 1.0
Which steps in glycolysis are irreversible?
A. Step 10
B. Step 8
C. Step 3
D. Step 7
E. Step 9
F. Step 6
G. Step 1
H. Step 4
I. Step 2
J. Step 5
A, C, G
What are the fates of pyruvate, the end-product of glycolysis? Select all that apply.
A. fermented to alcohol
B. converted to acetyl-CoA that enters the Kreb’s cycle
C. serves as precursor for some anabolic pathways
D. fermented to lactic acid
All choices.
How many ATP molecules are produced when 6 molecules of glucose enter glycolysis?
12
How many molecules of NADH are produced if 12 molecules of glucose enter the glycolytic pathway?
24
If 12 molecules of glucose enter the glycolytic pathway, how many ATP molecules can be generated in the electron transport chain from the NADH produced in the pathway assuming that there are 10 c subunits in the Fo complex of the organism?
55
Glycolysis converts one glucose molecule into what?
A) 2 ATP, 2 pyruvate, 2 NADH
B) 3 ATP, 2 pyruvate, 2 NADH
C) 2 ATP, 2 pyruvate, 10 NADH
D) 3 ATP, 2 pyruvate, 10 NADH
A) 2 ATP, 2 pyruvate, 2 NADH
True or False: The citric acid cycle occurs twice for each glucose molecule.
True
In the presence of oxygen, pyruvate is converted into what during pyruvate oxidation?
A) Lactate
B) Acetyl CoA
C) Oxaloacetate
D) Glucose
B) Acetyl CoA
True or False: The electron transport chain produces the majority of ATP in cellular respiration.
True
What is the terminal electron acceptor in the electron transport chain?
A) NADH
B) Oxygen
C) FADH2
D) Carbon dioxide
B) Oxygen
What is Saccharomyces cerevisiae commonly known as?
A) Brewer’s yeast
B) Baker’s yeast
C) Wine yeast
D) All of the above
D) All of the above
True or False: Sucrose is a disaccharide composed of glucose and fructose.
True
Which enzyme converts sucrose into glucose and fructose?
A) Amylase
B) Invertase
C) Maltase
D) Zymase
B) Invertase
True or False: Flour contains amylase which breaks up starch.
True
What is the expected result for a bottle setup with 200 mL water + 1 tbsp yeast?
A) Release CO2
B) No release of CO2
B) No release of CO2
In the absence of oxygen, what does yeast switch to for metabolism?
A) Respiration
B) Fermentation
B) Fermentation
True or False: Anaerobic respiration in yeast produces alcohol and CO2.
True
What enzyme converts pyruvate to acetaldehyde in yeast?
A) Alcohol dehydrogenase
B) Pyruvate decarboxylase
C) Maltase
D) Invertase
B) Pyruvate decarboxylase
Which enzyme is involved in the digestion of milk proteins in young mammals?
A) Pepsin
B) Renin
C) Amylase
D) Lipase
B) Renin