Mitochondrial

Question 1

What happens to mitochondria after stroke and myocardial infarction?

Answer 1

acute loss of them

Question 2

neurodegenaration is related too

Answer 2

loss of mito function

Question 3

outer membrane is composed of

Answer 3

50% protein

Question 4

inner membrane composed of

Answer 4

80% protein, cardiolipin

Question 5

outer membrane has _____ channels and is permeable to ________

Answer 5

outer membrane has porin channels and is permeable to small molecules (<10 kDA)

Question 6

inner membrane contains (3)

Answer 6

(1) complexes I-IV
(2) ATP synthase
(3) transporters (ANT)

Question 7

TCA cycle and beta-oxidation enzymes are

Answer 7

nuclear

Question 8

TOM

Answer 8

translocases of the outer membrane

Question 9

TIM

Answer 9

translocases of the inner membrane

Question 10

mitochondria contain ______ DNA

Answer 10

bacterial-like

Question 11

mitochondria has _____ compartments

Answer 11

4

Question 12

electron transfer by respiratory chain is couples with

Answer 12

the transfer of protons

Question 13

oxidative phosphorylation

Answer 13

production of ATP

Question 14

electron transfer from NADH to O2 involves

Answer 14

complexes I-IV

Question 15

generation of the electrochemical proton transmembrane potential involves

Answer 15

complexes I, III, IV

Question 16

use proton motive force to synthesize ATP

Answer 16

ATP synthase; complex V

Question 17

reduction potential reflects

Answer 17

its affinity for electrons

Question 18

final acceptor of electrons

Answer 18

O2

Question 19

respiratory chain consists of 4 protein complexes that use various _______ to shuttle electrons

Answer 19

prosthetic groups

Question 20

prosthetic group IV

Answer 20

heme

Question 21

rotenone, Amytal, Piericidin A inhibits

Answer 21

Complex I

Question 22

antimycin inhibts

Answer 22

complex III

Question 23

carbon monoxide, cyanide, azide inhibits

Answer 23

complex IV

Question 24

which complex is not a proton pump?

Answer 24

complex II

Question 25

oligomycin

Answer 25

inhibitor of ATP synthase

Question 26

the mitochondrial inner membrane is impermeable to

Answer 26

NADH/NAD+

Question 27

FADH2 transfers electrons to

Answer 27

Q

Question 28

irreversible shuttle

Answer 28

glycerol phosphate

Question 29

reversible shuttle

Answer 29

malate-aspartate

Question 30

coupling

Answer 30

rates of oxygen consumption by respiratory chain and ATP sythesis by ATP synthase are interdependent

Question 31

acute opening of mitochondrial transition pore leads to

Answer 31

inner membrane permeability transition- key to stroke and MI!

Question 32

mTDNA has no ____ and only codes for ___ proteins the other are encoded by nuclear DNA

Answer 32

introns and only codes fro 13 proteins

Question 33

energy dependent chaperones

Answer 33

TOM and TIM

Question 34

pre-sequence peptide in the protein

Answer 34

TOM

Question 35

internal sequence peptide in the pre-protein

Answer 35

TIM

Question 36

takes proteins inside

Answer 36

TOM

Question 37

cells create their own ___, cannot use dietary

Answer 37

ATP

Question 38

TCA cycle is an

Answer 38

oxidative process of Acetyl CoA

Question 39

Factoid of complexes and their organic complexes/ prosthetic groups

Answer 39

complexes do not participate in rxn it is their groups that do the m

Question 40

which complexes if inhibited stops respiration

Answer 40

Complexes III and IV

Question 41

IMM is impermeable to

Answer 41

NADH/NAD+

Question 42

how are the electrons of NADH transported?

Answer 42

Shuttles!!!

Question 43

Glycerol Phosphate shuttle is a ________ enzyme and it is an _____ process

Answer 43

it is a complex II enzyme and it is an irreversible process

Question 44

thermogenin protein/UCP1 in brown adipose tissue

Answer 44

physiologic uncoupling

Question 45

ANT exports ____ from matrix and imports ____

Answer 45

exports ATP and imports ADP.

Question 46

ANT also imports ____ and exports ____

Answer 46

imports Pi and exports OH-

Question 47

atractyloside and bongkrekic acid inhibits

Answer 47

ANT

Question 48

High calcium triggers _____ which is associated with stroke and MI.

Answer 48

MPT

Question 49

CsA binds to CypD and stops PT pores from opening leading to ____ being inhibited

Answer 49

MPT