Miscellaneous Protozoa Flashcards
Balanditum coli cyst
Size: _______
Number and appearance of nuclei for Balanditum coli cyst? ______
Size: 43-66um
Number and appearance of nuclei for Balanditum coli cyst: Two
• Kidney Shaped Macronucleus
• Small Spherical Micronucleus (May not be observable)
Other features for Balanditum coli cyst?
- One or two _____ contractile vacuoles in young cysts
- _____ cyst wall
- Row of ____ visible in between cyst wall layers of young cysts
- One or two visible contractile vacuoles in young cysts
- Double cyst wall
- Row of cilia visible in between cyst wall layers of young cysts
What is the size range for Balantidum coli trophozoite?
How many nuclei does Balantidum coli trophozoite has?
28-152um x 22-123;
Two
• Kidney-Shaped Macronucleus
• Small Spherical Micronucleus
What is the motility of Balantidum coli trophozoite?
Rotary, Boring
Other features for Balanditum coli trophozoite?
- One or two visible contractile vacuoles
- Cytoplasm may contain food _______ / _______
- _____ cytostome present
- Layer of _____ around organism
- One or two visible contractile vacuoles
- Cytoplasm may contain food vacuoles / bacteria
- Small cytostome present
- Layer of cilia around organism
What are the characteristics of Isospora belli oocyst?
I. SIZE RANGE: 25-35um x 10-15um
II. APPEARANCE: _______
III. SHAPE: _____
IV. CELL WALL: ______, colorless, smooth
V. DEVELOPING SPOROBLAST: _____ w/ Granular cytoplasm
VI. YOUNG OOCYST: ____ sporoblasts
VII. MATURE OOCYST: Two sporocysts, each has four ______-shaped sporozoites
I. SIZE RANGE: 25-35um x 10-15um
II. APPEARANCE: Transparent
III. SHAPE: Oval
IV. CELL WALL: Two-layered, colorless, smooth
V. DEVELOPING SPOROBLAST: Unicellular w/ Granular cytoplasm
VI. YOUNG OOCYST: Two sporoblasts
VII. MATURE OOCYST: Two sporocysts, each has four
sausage-shaped sporozoites
What are the characteristics of Cryptosporidium parvum oocyst?
I. SIZE RANGE: 4-6um
II. SHAPE: _______
III. NUMBER OF SPOROCYSTS: ____
IV. NUMBER OF SPOROZOITES: Four (Small) OTHER FEATURES:
_____ Cell Wall
One to ____ dark granules may be visible
I. SIZE RANGE: 4-6um II. SHAPE: Roundish III. NUMBER OF SPOROCYSTS: None IV. NUMBER OF SPOROZOITES: Four (Small) OTHER FEATURES: Thick Cell Wall One to six dark granules may be visible
What are the characteristics of Cryptosporidium parvum oocyst? I. SIZE RANGE: 4-6um II. SHAPE: \_\_\_\_\_\_ III. NUMBER OF SPOROCYSTS: \_\_\_\_\_ IV. NUMBER OF SPOROZOITES: \_\_\_\_ (Small) OTHER FEATURES: Thick Cell Wall One to six \_\_\_\_ granules may be visible
I. SIZE RANGE: 4-6um II. SHAPE: Roundish III. NUMBER OF SPOROCYSTS: None IV. NUMBER OF SPOROZOITES: Four (Small) OTHER FEATURES: Thick Cell Wall One to six dark granules may be visible
What are the characteristics of Blastocystis hominis vacuolated form?
I. SIZE: 5-32um
II. VACUOLE: _____ located, fluid-filled, almost 90% of the whole organism
III. CYTOPLASM: Appears as _____ around periphery
IV. NUCLEI: Two to ____ located in cytoplasm
I. SIZE: 5-32um
II. VACUOLE: Centrally located, fluid-filled, almost 90% of the whole organism
III. CYTOPLASM: Appears as ring around periphery
IV. NUCLEI: Two to four located in cytoplasm
What are the characteristics of Toxoplasma Gondii Tachyzoites?
I. GENERAL COMMENT: Actively multiplying
II. SIZE: 3-7 x 2-4
III. SHAPE: ____-shaped, often more rounded on one
end
IV. NUMBER OF NUCLEI: ____
V. OTHER FEATURES: contains a variety of organelles that are not readily visible
I. GENERAL COMMENT: Actively multiplying
II. SIZE: 3-7 x 2-4
III. SHAPE: Crescent-shaped, often more rounded on one
end
IV. NUMBER OF NUCLEI: One
V. OTHER FEATURES: contains a variety of organelles that are not readily visible
What are the characteristics of Toxoplasma Gondii bradyzoites?
I. GENERAL COMMENT: ___-growing
II. SIZE: Smaller than ____
III. PHYSICAL APPEARANCE: Similar to that of Tachyzoites
IV. OTHER FEATURES:
Hundreds to thousands of bradyzoites enclose themselves to form a cyst that may measure 12-100um in diameter
I. GENERAL COMMENT: Slow-growing
II. SIZE: Smaller than tachyzoites
III. PHYSICAL APPEARANCE: Similar to that of Tachyzoites
IV. OTHER FEATURES:
Hundreds to thousands of bradyzoites enclose themselves to form a cyst that may measure 12-100um in diameter
What are the characteristics of Sarcocystis spp. Mature Oocyst?
I. Shape: ____
II. Appearance: _____
III. Number of sporocysts: ____
IV. Size of each sporocyst: 10-18 um long
V. Contents of each sporocyst: ____ sausage-shaped sporozoites
VI. Oocyst cell wall appearance: Clear, ____, double-layered
I. Shape: Oval
II. Appearance: Transparent
III. Number of sporocysts: Two
IV. Size of each sporocyst: 10-18 um long
V. Contents of each sporocyst: Four sausage-shaped sporozoites
VI. Oocyst cell wall appearance: Clear, colorless, double-layered
What are the characteristics of Cyclosopora cayetanensis Mature oocyst?
I. Size: 7-10 um in diameter
II. Number of sporocysts: ____
III. Contents of sporocysts: Each sporocyst contains two sporozoites
I. Size: 7-10 um in diamete
II. Number of sporocysts: Two
III. Contents of sporocysts: Each sporocyst contains two sporozoites
What are the characteristics of Microsporidia Spore?
I. Size: 1-5 um
II. Other features: Equipped with extruding ____ filaments (or tubules) that initiate infection by injecting sporoplasm (infectious material) into host cell
I. Size: 1-5 um
II. Other features: Equipped with extruding polar filaments (or tubules) that initiate infection by injecting sporoplasm (infectious material) into host cell
What are the characteristics for Pneumocystis jiroveci cyst?
I. Size: Diameter, 4-12 um
II. Shape: _____
III. Number of nuclei: ____ to eight; _____ or arranged in _____
I. Size: Diamete, 4-12 um
II. Shape: Roundish
III. Number of nuclei: Four to eight; unorganized or arranged in rosette
