Miscellaneous Protozoa Flashcards
Balanditum coli cyst
Size: _______
Number and appearance of nuclei for Balanditum coli cyst? ______
Size: 43-66um
Number and appearance of nuclei for Balanditum coli cyst: Two
• Kidney Shaped Macronucleus
• Small Spherical Micronucleus (May not be observable)
Other features for Balanditum coli cyst?
- One or two _____ contractile vacuoles in young cysts
- _____ cyst wall
- Row of ____ visible in between cyst wall layers of young cysts
- One or two visible contractile vacuoles in young cysts
- Double cyst wall
- Row of cilia visible in between cyst wall layers of young cysts
What is the size range for Balantidum coli trophozoite?
How many nuclei does Balantidum coli trophozoite has?
28-152um x 22-123;
Two
• Kidney-Shaped Macronucleus
• Small Spherical Micronucleus
What is the motility of Balantidum coli trophozoite?
Rotary, Boring
Other features for Balanditum coli trophozoite?
- One or two visible contractile vacuoles
- Cytoplasm may contain food _______ / _______
- _____ cytostome present
- Layer of _____ around organism
- One or two visible contractile vacuoles
- Cytoplasm may contain food vacuoles / bacteria
- Small cytostome present
- Layer of cilia around organism
What are the characteristics of Isospora belli oocyst?
I. SIZE RANGE: 25-35um x 10-15um
II. APPEARANCE: _______
III. SHAPE: _____
IV. CELL WALL: ______, colorless, smooth
V. DEVELOPING SPOROBLAST: _____ w/ Granular cytoplasm
VI. YOUNG OOCYST: ____ sporoblasts
VII. MATURE OOCYST: Two sporocysts, each has four ______-shaped sporozoites
I. SIZE RANGE: 25-35um x 10-15um
II. APPEARANCE: Transparent
III. SHAPE: Oval
IV. CELL WALL: Two-layered, colorless, smooth
V. DEVELOPING SPOROBLAST: Unicellular w/ Granular cytoplasm
VI. YOUNG OOCYST: Two sporoblasts
VII. MATURE OOCYST: Two sporocysts, each has four
sausage-shaped sporozoites
What are the characteristics of Cryptosporidium parvum oocyst?
I. SIZE RANGE: 4-6um
II. SHAPE: _______
III. NUMBER OF SPOROCYSTS: ____
IV. NUMBER OF SPOROZOITES: Four (Small) OTHER FEATURES:
_____ Cell Wall
One to ____ dark granules may be visible
I. SIZE RANGE: 4-6um II. SHAPE: Roundish III. NUMBER OF SPOROCYSTS: None IV. NUMBER OF SPOROZOITES: Four (Small) OTHER FEATURES: Thick Cell Wall One to six dark granules may be visible
What are the characteristics of Cryptosporidium parvum oocyst? I. SIZE RANGE: 4-6um II. SHAPE: \_\_\_\_\_\_ III. NUMBER OF SPOROCYSTS: \_\_\_\_\_ IV. NUMBER OF SPOROZOITES: \_\_\_\_ (Small) OTHER FEATURES: Thick Cell Wall One to six \_\_\_\_ granules may be visible
I. SIZE RANGE: 4-6um II. SHAPE: Roundish III. NUMBER OF SPOROCYSTS: None IV. NUMBER OF SPOROZOITES: Four (Small) OTHER FEATURES: Thick Cell Wall One to six dark granules may be visible
What are the characteristics of Blastocystis hominis vacuolated form?
I. SIZE: 5-32um
II. VACUOLE: _____ located, fluid-filled, almost 90% of the whole organism
III. CYTOPLASM: Appears as _____ around periphery
IV. NUCLEI: Two to ____ located in cytoplasm
I. SIZE: 5-32um
II. VACUOLE: Centrally located, fluid-filled, almost 90% of the whole organism
III. CYTOPLASM: Appears as ring around periphery
IV. NUCLEI: Two to four located in cytoplasm
What are the characteristics of Toxoplasma Gondii Tachyzoites?
I. GENERAL COMMENT: Actively multiplying
II. SIZE: 3-7 x 2-4
III. SHAPE: ____-shaped, often more rounded on one
end
IV. NUMBER OF NUCLEI: ____
V. OTHER FEATURES: contains a variety of organelles that are not readily visible
I. GENERAL COMMENT: Actively multiplying
II. SIZE: 3-7 x 2-4
III. SHAPE: Crescent-shaped, often more rounded on one
end
IV. NUMBER OF NUCLEI: One
V. OTHER FEATURES: contains a variety of organelles that are not readily visible
What are the characteristics of Toxoplasma Gondii bradyzoites?
I. GENERAL COMMENT: ___-growing
II. SIZE: Smaller than ____
III. PHYSICAL APPEARANCE: Similar to that of Tachyzoites
IV. OTHER FEATURES:
Hundreds to thousands of bradyzoites enclose themselves to form a cyst that may measure 12-100um in diameter
I. GENERAL COMMENT: Slow-growing
II. SIZE: Smaller than tachyzoites
III. PHYSICAL APPEARANCE: Similar to that of Tachyzoites
IV. OTHER FEATURES:
Hundreds to thousands of bradyzoites enclose themselves to form a cyst that may measure 12-100um in diameter
What are the characteristics of Sarcocystis spp. Mature Oocyst?
I. Shape: ____
II. Appearance: _____
III. Number of sporocysts: ____
IV. Size of each sporocyst: 10-18 um long
V. Contents of each sporocyst: ____ sausage-shaped sporozoites
VI. Oocyst cell wall appearance: Clear, ____, double-layered
I. Shape: Oval
II. Appearance: Transparent
III. Number of sporocysts: Two
IV. Size of each sporocyst: 10-18 um long
V. Contents of each sporocyst: Four sausage-shaped sporozoites
VI. Oocyst cell wall appearance: Clear, colorless, double-layered
What are the characteristics of Cyclosopora cayetanensis Mature oocyst?
I. Size: 7-10 um in diameter
II. Number of sporocysts: ____
III. Contents of sporocysts: Each sporocyst contains two sporozoites
I. Size: 7-10 um in diamete
II. Number of sporocysts: Two
III. Contents of sporocysts: Each sporocyst contains two sporozoites
What are the characteristics of Microsporidia Spore?
I. Size: 1-5 um
II. Other features: Equipped with extruding ____ filaments (or tubules) that initiate infection by injecting sporoplasm (infectious material) into host cell
I. Size: 1-5 um
II. Other features: Equipped with extruding polar filaments (or tubules) that initiate infection by injecting sporoplasm (infectious material) into host cell
What are the characteristics for Pneumocystis jiroveci cyst?
I. Size: Diameter, 4-12 um
II. Shape: _____
III. Number of nuclei: ____ to eight; _____ or arranged in _____
I. Size: Diamete, 4-12 um
II. Shape: Roundish
III. Number of nuclei: Four to eight; unorganized or arranged in rosette
What are the characteristics for Pneumocystis jiroveci trophozoite?
I. Size: 2-4 um
II. Shape: Ovoid, _______
III. Number of nuclei: ____
I. Size: 2-4 um
II. Shape: Ovoid, ameboid
III. Number of nuclei: One
Common associated disease for Balanditum coli?
Balantidiasis
Common associated disease for Isospora belli?
Isosporiasis
Common associated disease for Cryptosporidium parvum?
Cryptosporidosis
Common associated disease for Blastocystis hominis?
Blastocystis hominis infection
Common associated disease for Toxoplasma gondii?
Toxoplasmosis
Congenital toxoplasmosis
Cerebral toxoplasmosis
Common associated disease for Pneumocystis jirovenci (carinii)?
Pneumocystosis
Atypical interstitial plasma cell pneumonia
Common associated disease for Microsporidia?
Microsporidia infection
Microsporidial infection
Common associated disease for Sarcocystis spp?
Sarcocystis infection
