MIDTERM LAB 1: DFS

Question 1

DIRECT FECAL SMEAR OR?

Answer 1

DIRECT WET MOUNT

Question 2

• simplest and easiest technique
• need to use permanent dyes
• Slide made by mixing a small portion of
  unfixed stool with saline or iodine and
  subsequent examination of the resultant
  mixture under the microscope is to detect
  the presence of motile protozoan
  trophozoite

Answer 2

DIRECT FECAL SMEAR

Question 3

A tool used to measure cysts and ova, will be useful in specific species identification

Answer 3

MICROMETRY

Question 4

2 REAGENTS USE IN DFS

Answer 4

• 0.85% NORMAL SALINE SOLUTION (NSS)
• LUGOL’S IODINE SOLUTION

Question 5

• helps visualize the motility of an organism
• designed to be isotonic (equal concentration of solution/ cell will not burst or shrink)
• BEST FOR WATERY STOOLS

Answer 5

0.85% NORMAL SALINE SOLUTION (NSS)

Question 6

use to reveal/stain parasite structure
- CANNOT be used to visualize trophozoites (trophozoites can be kill)
- no need to dilute

Answer 6

LUGOL’S IODINE SOLUTION

Question 7

• concen. of solute is GREATER than conc. of cell
• cell will SHRINK

Answer 7

HYPERTONIC

Question 8

• concen. of solute is LESS than conc. of cell
• cell will BURST

Answer 8

HYPOTONIC

Question 9

materials

Answer 9

• GLASS SLIDES
• APPLICATOR STICK
• DROPPER
• 0.85% NORMAL SALINE SOLUTION (NSS)
• LUGOL’S IODINE SOLUTION
• STOOL SAMPLE (PEA-SIZED)