Microscopy & Measurement (PPT 2) (U1) Flashcards
1
Q
What does magnification refer to?
A
To how much larger a sample appears to be compared to its actual size.
2
Q
As the —– increases, the field of view ——-.
A
1- Magnification
2- Decreases
3
Q
What are the 3 methods to calculate the magnification?
A
1- The ocular and objective lenses
2- The scale bar
3- The image provided they give the actual size
4
Q
How do you calculate the Mag using the lens?
A
- Multiply the Mag of the ocular lens by the mag of the objective lens
5
Q
Scanning power=??
Low power=??
High power=??
A
Sp= 10 x 4 Lp= 10 x 10 Hp= 10 x 40
6
Q
1mm = ? micrometers 1cm = ? millimetres 1cm = ?micrometers 1micro= ? nanometers
A
1mm= 1000 micros 1cm = 10mm 1cm = 10,000 micros 1microm = 1000nm
7
Q
M= I/A means??
A
Magnification = Observed, measured/ Actual
