Microscopy

Question 1

What do microscopes allow us to do

Answer 1

See things we can’t see with the naked eye

Question 2

Name and describe the two types of microscopes

Answer 2

Light - use light and lenses to form an image of a specimen and magnify if - make individual cells and subcellular cells visable
Electron - use electrons - higher magnification - higher resolution - mitochondria, chloroplasts, ribosomes and plasmids

Question 3

What is the equation for magnification

Answer 3

image size / real size

Question 4

Give instructions on how to prepare your slide

Answer 4

• add a drop of water to the middle of the slide
• using tweezers place the thing your studying onto the water
• add a drop of iodine solution
• place a cover strip onto the slide without getting any air bubbles

Question 5

Name the parts of a light microscope

Answer 5

Eyepiece
Coarse adjustment knob
Fine adjustment knob
Light
Stage
High and low power objective lenses