Microscopy

Question 1

Describe how to prepare a specimen

Answer 1

Put the cells on the slide
Put the crystal violet stain on the slide
Add Mordant Grams iodine
Add decolorizer alcohol/acetone
Put the safranin counterstain on the slide

Question 2

List the organisms that stain positive in acid-fast stains

Answer 2

Mycobacteria
Nocardia
Cryptospirida
Cyclospora

Question 3

Four types of fungal stains

Answer 3

Lactophenol cotton blue
Gomori methenamine silver
Periodic acid-Shiff
Mucicarmine

Question 4

Organisms identified with lactophenol cotton blue

Answer 4

Histoplasma

Question 5

Organisms identified with Gomori methenamine silver

Answer 5

Yeast cells and hyphae, Pneumocystitis carnii

Question 6

Organisms identified with periodic acid-Shiff

Answer 6

Yeast cells and hyphae

Question 7

Organisms identified with mucicarmine

Answer 7

Cryptococcus capsule

Question 8

Four types of parasitology stains

Answer 8

Wright’s giemsa
Wet mounts
Trichome
Modified acid fast

Question 9

Organisms identified with Wright’s Giemsa

Answer 9

Peripheral blood smear for histoplasma, leishmania, trypanosomas, and malaria

Question 10

Organisms identified with wet mounts

Answer 10

Giardia

Question 11

Organisms identified with trichrome staining

Answer 11

Giardia

Question 12

Organisms identified with modified acid fast

Answer 12

Cryptosporidium

Question 13

Staphylococcus aureus

Answer 13

Clumps

Question 14

Neisseria meningitidis

Answer 14

Little diplococci that invade

Question 15

Streptococcus pneumonia

Answer 15

Diplococci

Question 16

E. coli

Answer 16

Pink, small clumped rods

Question 17

Actinomyces

Answer 17

Look like little dark tree branches

Question 18

Campylobacter

Answer 18

Little rods all over the place

Question 19

Nocardia

Answer 19

Red, artery-looking