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MICROSCOPY Flashcards

1
Q

A _____________is an instrument that can be used to observe small objects, even cells. The image of an object is magnified through at least one lens in the microscope. This lens bends light toward the eye and makes an object appear larger than it actually is

A

microscope

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2
Q

1 µm micrometer = _____ m = _____mm

A

10-6 m = 10-3 mm

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3
Q

1 nm nanometer = _____ m = ____ mm

A

10-9m = 10-6mm

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4
Q

______ nm = 1 µm

A

1000nm

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5
Q

______µm = 1 nm

A

0.001µm

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6
Q

______ microscope has only one lens.

A

simple microscope

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7
Q

sections that mostly uses microscope

A

clinical microscopy and hematology

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8
Q

In a __________ the image from the objective lens is magnified again by the ocular lens.

A

compound microscope

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9
Q

Total magnification = _______ x _______

A

objective lens x ocular lens

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10
Q

it is the most used in the modern laboratory

A

Compound microscope

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11
Q

this is where the ocular lenses are attached

A

Body tube

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12
Q

____________ primarily attached to the revolving nosepiece

A

Objective lens

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13
Q

__________ is the first objective lens used before the other objective lenses

A

Scanner

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14
Q

___________ the three main objective lenses used in compound microscope

A

LPO, HPO and OIO

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15
Q

________ & ________ is where the light are adjusted

A

Condenser & Iris diaphragm

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16
Q

it is the light source

A

Illuminator

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17
Q

When lifting a microscope, you should carry it by holding the ___________ & ___________

A

arm and the base

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18
Q

The secret technique is _________________ when trying to vew

A

not blinking too much

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19
Q

____ ______ must be kept open when viewing

A

Two eyes

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20
Q

__________________ microscope with two ocular lenses.

A

Binocular compound light microscope

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21
Q

The standard magnification of the ocular lens _______

A

10x

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22
Q

Low Power Objective Magnification

A

10x

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23
Q

High Power Objective Magnification

A

40x

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24
Q

Oil Immersion Objective Magnification

A

100x

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25
_______ is the ability of the lenses to distinguish two points.
Resolution
26
Shorter wavelengths of light provide _________ resolution.
greater resolution
27
____________ is the light-bending ability of a medium.
Refractive index
28
____________ is used to keep light from bending.
Immersion oil
29
Utilized transmitted light
Brightfield Illumination
30
Dark objects are visible against a bright background.
Bright field Microscope
31
Light reflected off the specimen does not enter the objective lens.
Bright field Microscope
32
Ultilized reflected light with a special condenser
Darkfield Illumination/ Darkfield Microscope
33
Light objects are visible against a dark background.
Darkfield Illumination/ Darkfield Microscope
34
A microscope with a resolving power of 0.4 nm can distinguish between two points ≥ 0.4 nm.
≥ 0.4 nm
35
The light may bend in air so much that it misses the small high-magnification lens TRUE OR FALSE
TRUE
36
identify the microscope mentioned: Binary fission, motility and endospore
phase-contrast microscope
37
identify the microscope mentioned Direct and reflected light rays are combined at the eye. Increasing contrast
Phase-Contrast Microscopy
38
identify the microscope mentioned Accentuates diffraction of the light that passes through a specimen
phase-contrast microscope
39
______________ is the slight bending of light as it passes around the edge of an object.
Diffraction
40
______________ is the bending of a wave when it enters a medium where its speed is different
Refraction
41
type of microscope that uses opaque disc and special condenser
dark field microscope
42
type of microscope that uses annular diaphragm
phase-contrast microscope
43
microscope where only the specimen is illuminated
dark field microscope
44
type of microscope where light only reaches the sides of the specimen
dark field microscope
45
microscope where may be used to visualize live samples and avoid distortion from cell stain
phase-contrast microscope
46
type of microscope that uses dual beam optical system as light
differential interference contrast microscope
47
type of microscope that accentuates diffraction of the light that passes through a specimen, similar to phase-contrast microscope
differential interference contrast microscope
48
type of microscope that uses two beams of light. Adding color
differential interference contrast microscope
49
type of microscope that has better contrast
differential interference contrast microscope
50
type of microscopy that uses UV light
Fluorescence Microscopy
51
type of microscopy where Fluorescent substances absorb UV light and emit visible light
Fluorescence Microscopy
52
type of microscopy where cells may be stained with fluorescent dyes (fluorochromes).
Fluorescence Microscopy
53
type of microscopy which can detect antigen and antibodies
Fluorescence Microscopy
54
type of microscopy that uses UV light that has different nm compare to commonly used lights
Fluorescence Microscopy
55
type of microscopy that can greatly increase the resolution of cells and cell structures
Fluorescence Microscopy
56
type of microscopy that uses fluorochromes and a laser light
Confocal Microscopy
57
The laser illuminates each plane in a specimen to produce a 3-D image. which type of microscope is mentioned?
confocal microscope
58
type of microscopy where vertical and horizontal cross section are examined
Confocal Microscopy
59
type of microscopy where the inside details of the structure is viewed
Confocal Microscopy
60
type of microscopy that uses electrons instead of light
electron Microscopy
61
The shorter wavelength of electrons gives ___________ resolution.
greater
62
type of microscopy: Ultrathin sections of specimens. used in histopathology section
Transmission Electron Microscopy
63
type of microscopy: Light passes through specimen, then an electromagnetic lens, to a screen or film.
Transmission Electron Microscopy
64
type of microscopy: Specimens may be stained with heavy metal salts.
Transmission Electron Microscopy
65
type of microscopy: 10,000-100,000; resolution 2.5 nm
Transmission Electron Microscopy
66
type of microscopy: An electron gun produces a beam of electrons that scans the surface of a whole specimen.
Scanning Electron Microscopy
67
type of microscopy: Secondary electrons emitted from the specimen produce the image.
Scanning Electron Microscopy
68
type of microscopy: 1000-10,000; resolution 20 nm
Scanning Electron Microscopy
69
type of microscopy: Scanning tunneling microscopy uses a metal probe to scan a specimen.
Scanning-Probe Microscopy
70
type of microscopy: Resolution 1/100 of an atom
Scanning-Probe Microscopy
71
type of microscopy: Atomic force microscopy uses a metal and diamond probe inserted into the specimen.
Scanning-Probe Microscopy
72
Atomic force microscopy uses a ___________ and ____________ inserted into the specimen.
metal and diamond probe
73
type of microscopy: Produces 3-D images.
Scanning-Probe Microscopy

MLSP (9 decks)

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