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MLSP > MICROSCOPY > Flashcards

MICROSCOPY Flashcards

1
Q

A _____________is an instrument that can be used to observe small objects, even cells. The image of an object is magnified through at least one lens in the microscope. This lens bends light toward the eye and makes an object appear larger than it actually is

A

microscope

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2
Q

1 µm micrometer = _____ m = _____mm

A

10-6 m = 10-3 mm

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3
Q

1 nm nanometer = _____ m = ____ mm

A

10-9m = 10-6mm

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4
Q

______ nm = 1 µm

A

1000nm

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5
Q

______µm = 1 nm

A

0.001µm

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6
Q

______ microscope has only one lens.

A

simple microscope

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7
Q

sections that mostly uses microscope

A

clinical microscopy and hematology

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8
Q

In a __________ the image from the objective lens is magnified again by the ocular lens.

A

compound microscope

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9
Q

Total magnification = _______ x _______

A

objective lens x ocular lens

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10
Q

it is the most used in the modern laboratory

A

Compound microscope

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11
Q

this is where the ocular lenses are attached

A

Body tube

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12
Q

____________ primarily attached to the revolving nosepiece

A

Objective lens

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13
Q

__________ is the first objective lens used before the other objective lenses

A

Scanner

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14
Q

___________ the three main objective lenses used in compound microscope

A

LPO, HPO and OIO

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15
Q

________ & ________ is where the light are adjusted

A

Condenser & Iris diaphragm

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16
Q

it is the light source

A

Illuminator

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17
Q

When lifting a microscope, you should carry it by holding the ___________ & ___________

A

arm and the base

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18
Q

The secret technique is _________________ when trying to vew

A

not blinking too much

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19
Q

____ ______ must be kept open when viewing

A

Two eyes

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20
Q

__________________ microscope with two ocular lenses.

A

Binocular compound light microscope

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21
Q

The standard magnification of the ocular lens _______

A

10x

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22
Q

Low Power Objective Magnification

A

10x

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23
Q

High Power Objective Magnification

A

40x

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24
Q

Oil Immersion Objective Magnification

A

100x

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25
Q

_______ is the ability of the lenses to distinguish two points.

A

Resolution

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26
Q

Shorter wavelengths of light provide _________ resolution.

A

greater resolution

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27
Q

____________ is the light-bending ability of a medium.

A

Refractive index

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28
Q

____________ is used to keep light from bending.

A

Immersion oil

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29
Q

Utilized transmitted light

A

Brightfield Illumination

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30
Q

Dark objects are visible against a bright background.

A

Bright field Microscope

31
Q

Light reflected off the specimen does not enter the objective lens.

A

Bright field Microscope

32
Q

Ultilized reflected light with a special condenser

A

Darkfield Illumination/ Darkfield Microscope

33
Q

Light objects are visible against a dark background.

A

Darkfield Illumination/ Darkfield Microscope

34
Q

A microscope with a resolving power of 0.4 nm can distinguish between two points ≥ 0.4 nm.

A

≥ 0.4 nm

35
Q

The light may bend in air so much that it misses the small high-magnification lens

TRUE OR FALSE

A

TRUE

36
Q

identify the microscope mentioned:

Binary fission, motility and endospore

A

phase-contrast microscope

37
Q

identify the microscope mentioned

Direct and reflected light rays are combined at the eye. Increasing contrast

A

Phase-Contrast Microscopy

38
Q

identify the microscope mentioned

Accentuates diffraction of the light that passes through a specimen

A

phase-contrast microscope

39
Q

______________ is the slight bending of light as it passes around the edge of an object.

A

Diffraction

40
Q

______________ is the bending of a wave when it enters a medium where its speed is different

A

Refraction

41
Q

type of microscope that uses opaque disc and special condenser

A

dark field microscope

42
Q

type of microscope that uses annular diaphragm

A

phase-contrast microscope

43
Q

microscope where only the specimen is illuminated

A

dark field microscope

44
Q

type of microscope where light only reaches the sides of the specimen

A

dark field microscope

45
Q

microscope where may be used to visualize live samples and avoid distortion from cell stain

A

phase-contrast microscope

46
Q

type of microscope that uses dual beam optical system as light

A

differential interference contrast microscope

47
Q

type of microscope that accentuates diffraction of the light that passes through a specimen, similar to phase-contrast microscope

A

differential interference contrast microscope

48
Q

type of microscope that uses two beams of light. Adding color

A

differential interference contrast microscope

49
Q

type of microscope that has better contrast

A

differential interference contrast microscope

50
Q

type of microscopy that uses UV light

A

Fluorescence Microscopy

51
Q

type of microscopy where Fluorescent substances absorb UV light and emit visible light

A

Fluorescence Microscopy

52
Q

type of microscopy where cells may be stained with fluorescent dyes (fluorochromes).

A

Fluorescence Microscopy

53
Q

type of microscopy which can detect antigen and antibodies

A

Fluorescence Microscopy

54
Q

type of microscopy that uses UV light that has different nm compare to commonly used lights

A

Fluorescence Microscopy

55
Q

type of microscopy that can greatly increase the resolution of cells and cell structures

A

Fluorescence Microscopy

56
Q

type of microscopy that uses fluorochromes and a laser light

A

Confocal Microscopy

57
Q

The laser illuminates each plane in a specimen to produce a 3-D image. which type of microscope is mentioned?

A

confocal microscope

58
Q

type of microscopy where vertical and horizontal cross section are examined

A

Confocal Microscopy

59
Q

type of microscopy where the inside details of the structure is viewed

A

Confocal Microscopy

60
Q

type of microscopy that uses electrons instead of light

A

electron Microscopy

61
Q

The shorter wavelength of electrons gives ___________ resolution.

A

greater

62
Q

type of microscopy:

Ultrathin sections of specimens. used in histopathology section

A

Transmission Electron Microscopy

63
Q

type of microscopy:

Light passes through specimen, then an electromagnetic lens, to a screen or film.

A

Transmission Electron Microscopy

64
Q

type of microscopy:

Specimens may be stained with heavy metal salts.

A

Transmission Electron Microscopy

65
Q

type of microscopy:

10,000-100,000; resolution 2.5 nm

A

Transmission Electron Microscopy

66
Q

type of microscopy:

An electron gun produces a beam of electrons that scans the surface of a whole specimen.

A

Scanning Electron Microscopy

67
Q

type of microscopy:

Secondary electrons emitted from the specimen produce the image.

A

Scanning Electron Microscopy

68
Q

type of microscopy:

1000-10,000; resolution 20 nm

A

Scanning Electron Microscopy

69
Q

type of microscopy:

Scanning tunneling microscopy uses a metal probe to scan a specimen.

A

Scanning-Probe Microscopy

70
Q

type of microscopy:

Resolution 1/100 of an atom

A

Scanning-Probe Microscopy

71
Q

type of microscopy:

Atomic force microscopy uses a metal and diamond probe inserted into the specimen.

A

Scanning-Probe Microscopy

72
Q

Atomic force microscopy uses a ___________ and ____________ inserted into the specimen.

A

metal and diamond probe

73
Q

type of microscopy:

Produces 3-D images.

A

Scanning-Probe Microscopy

MLSP (9 decks)

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