MICROSCOPY Flashcards
A _____________is an instrument that can be used to observe small objects, even cells. The image of an object is magnified through at least one lens in the microscope. This lens bends light toward the eye and makes an object appear larger than it actually is
microscope
1 µm micrometer = _____ m = _____mm
10-6 m = 10-3 mm
1 nm nanometer = _____ m = ____ mm
10-9m = 10-6mm
______ nm = 1 µm
1000nm
______µm = 1 nm
0.001µm
______ microscope has only one lens.
simple microscope
sections that mostly uses microscope
clinical microscopy and hematology
In a __________ the image from the objective lens is magnified again by the ocular lens.
compound microscope
Total magnification = _______ x _______
objective lens x ocular lens
it is the most used in the modern laboratory
Compound microscope
this is where the ocular lenses are attached
Body tube
____________ primarily attached to the revolving nosepiece
Objective lens
__________ is the first objective lens used before the other objective lenses
Scanner
___________ the three main objective lenses used in compound microscope
LPO, HPO and OIO
________ & ________ is where the light are adjusted
Condenser & Iris diaphragm
it is the light source
Illuminator
When lifting a microscope, you should carry it by holding the ___________ & ___________
arm and the base
The secret technique is _________________ when trying to vew
not blinking too much
____ ______ must be kept open when viewing
Two eyes
__________________ microscope with two ocular lenses.
Binocular compound light microscope
The standard magnification of the ocular lens _______
10x
Low Power Objective Magnification
10x
High Power Objective Magnification
40x
Oil Immersion Objective Magnification
100x
_______ is the ability of the lenses to distinguish two points.
Resolution
Shorter wavelengths of light provide _________ resolution.
greater resolution
____________ is the light-bending ability of a medium.
Refractive index
____________ is used to keep light from bending.
Immersion oil
Utilized transmitted light
Brightfield Illumination
Dark objects are visible against a bright background.
Bright field Microscope
Light reflected off the specimen does not enter the objective lens.
Bright field Microscope
Ultilized reflected light with a special condenser
Darkfield Illumination/ Darkfield Microscope
Light objects are visible against a dark background.
Darkfield Illumination/ Darkfield Microscope
A microscope with a resolving power of 0.4 nm can distinguish between two points ≥ 0.4 nm.
≥ 0.4 nm
The light may bend in air so much that it misses the small high-magnification lens
TRUE OR FALSE
TRUE
identify the microscope mentioned:
Binary fission, motility and endospore
phase-contrast microscope
identify the microscope mentioned
Direct and reflected light rays are combined at the eye. Increasing contrast
Phase-Contrast Microscopy
identify the microscope mentioned
Accentuates diffraction of the light that passes through a specimen
phase-contrast microscope
______________ is the slight bending of light as it passes around the edge of an object.
Diffraction
______________ is the bending of a wave when it enters a medium where its speed is different
Refraction
type of microscope that uses opaque disc and special condenser
dark field microscope
type of microscope that uses annular diaphragm
phase-contrast microscope
microscope where only the specimen is illuminated
dark field microscope
type of microscope where light only reaches the sides of the specimen
dark field microscope
microscope where may be used to visualize live samples and avoid distortion from cell stain
phase-contrast microscope
type of microscope that uses dual beam optical system as light
differential interference contrast microscope
type of microscope that accentuates diffraction of the light that passes through a specimen, similar to phase-contrast microscope
differential interference contrast microscope
type of microscope that uses two beams of light. Adding color
differential interference contrast microscope
type of microscope that has better contrast
differential interference contrast microscope
type of microscopy that uses UV light
Fluorescence Microscopy
type of microscopy where Fluorescent substances absorb UV light and emit visible light
Fluorescence Microscopy
type of microscopy where cells may be stained with fluorescent dyes (fluorochromes).
Fluorescence Microscopy
type of microscopy which can detect antigen and antibodies
Fluorescence Microscopy
type of microscopy that uses UV light that has different nm compare to commonly used lights
Fluorescence Microscopy
type of microscopy that can greatly increase the resolution of cells and cell structures
Fluorescence Microscopy
type of microscopy that uses fluorochromes and a laser light
Confocal Microscopy
The laser illuminates each plane in a specimen to produce a 3-D image. which type of microscope is mentioned?
confocal microscope
type of microscopy where vertical and horizontal cross section are examined
Confocal Microscopy
type of microscopy where the inside details of the structure is viewed
Confocal Microscopy
type of microscopy that uses electrons instead of light
electron Microscopy
The shorter wavelength of electrons gives ___________ resolution.
greater
type of microscopy:
Ultrathin sections of specimens. used in histopathology section
Transmission Electron Microscopy
type of microscopy:
Light passes through specimen, then an electromagnetic lens, to a screen or film.
Transmission Electron Microscopy
type of microscopy:
Specimens may be stained with heavy metal salts.
Transmission Electron Microscopy
type of microscopy:
10,000-100,000; resolution 2.5 nm
Transmission Electron Microscopy
type of microscopy:
An electron gun produces a beam of electrons that scans the surface of a whole specimen.
Scanning Electron Microscopy
type of microscopy:
Secondary electrons emitted from the specimen produce the image.
Scanning Electron Microscopy
type of microscopy:
1000-10,000; resolution 20 nm
Scanning Electron Microscopy
type of microscopy:
Scanning tunneling microscopy uses a metal probe to scan a specimen.
Scanning-Probe Microscopy
type of microscopy:
Resolution 1/100 of an atom
Scanning-Probe Microscopy
type of microscopy:
Atomic force microscopy uses a metal and diamond probe inserted into the specimen.
Scanning-Probe Microscopy
Atomic force microscopy uses a ___________ and ____________ inserted into the specimen.
metal and diamond probe
type of microscopy:
Produces 3-D images.
Scanning-Probe Microscopy
