Microscopes Flashcards

0
Q

Total magnification

A

The enlarged size of the object verses it’s actual size when viewed using both ocular and objective lenses

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1
Q

Objective lenses

A

The 4 different lenses used to view the object in 4 different magnifications. 40x 200x, 400x, 600x

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2
Q

Magnification of ocular lense

A

10x

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3
Q

Magnification of scanning power lens

A

4x

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4
Q

Magnification of low power lens

A

20x

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5
Q

Magnification of medium power lens

A

40x

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6
Q

Magnification of high power lens

A

60x

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7
Q

Scanning power total magnification

A

40x

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8
Q

Low power total magnification

A

200x

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9
Q

Medium power total magnification

A

400x

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10
Q

High power total magnification

A

600x

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11
Q

The microscopes used in class are parfocal meaning…

A

As you change the objective lenses, the object will remain mostly in focus

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12
Q

Resolution

A

How clear an image of an object appears.

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13
Q

Contrast

A

Determining different parts of the object

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14
Q

Field of view

A

The area that can be seen with the microscope

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15
Q

Orientation

A

The image of the object is the result of mirrors, causing the image to be a mirror image of the object.

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16
Q

First thing you do before getting a microscope…

A

Clear a space in your work area

17
Q

When carrying a microscope…

A

Always use two hands. Support the arm with one hand, and the base with the other hand

18
Q

What is the only acceptable thing to clean a lens with?

A

Lens paper

19
Q

Always start on what power when using a microscope..

A

Scanning power

20
Q

What power should the microscope be on when you put it away?

A

Scanning power

21
Q

Never use coarse adjustment knob when?

A

When using medium and high power

22
Q

You may use which microscope

A

Only your assigned one #18

23
Q

Which objective lens will give you the largest field of view?

A

Scanning objective lens

24
Q

Which objective lens do you always start viewing an object with?

A

Scanning objective lens

25
Q

Which objective lenses do you use the fine focus knob with?

A

Medium and high power objective lens. Because lenses are very close to the slides.

26
Q

Revolving nosepiece

A

The objective lenses are attached to the nose peice. Which allows you to change the objective lens in use. Rotate the nosepiece slowly, you will hear and feel a click when it is correctly in place

27
Q

Arm

A

The part to hold when carrying a microscope

28
Q

Base

A

Other hand is placed under the base when carrying, this part rests on the table

29
Q

Coarse adjustment focus knob

A

A large knob on either side of the microscope. Used to move the stage up and down, to position the object in focus. This knob should be used only when the microscope is on the scanning or low power objective lenses

30
Q

Fine adjustment focus knob

A

The smaller knob projecting out of the coarse adjustment knob. Makes very slight changes, used to bring the image of an object into sharp focus. Used on medium and high power objective lenses

31
Q

Stage

A

The place where the slide is placed for viewing. There is a hole in the stage; the object to be viewed should be placed directly over the hole to allow light to shine through the object.

32
Q

Stage clips and knobs

A

The metal clips holds the slide into place, and allow the slide to be moved easily to position or reposition an object

33
Q

Condenser

A

The condenser focuses light

34
Q

Iris diaphragm

A

This is located immediately under the stage. By moving this lever to the right or left, you can adjust the amt. of light that passes through the specimen and therefore the contrast

35
Q

Light source

A

Comes from the base of the microscope, shines upward to illuminate an object

36
Q

Reastat

A

Controls light. Little wheel. Needs to be turned away (all the way down) before putting the microscope back

37
Q

Total magnification is figured out how?

A

Total mag.= ocular mag x object. mag

38
Q

Pointer

A

The little needle that points to structures in the magnified image.

39
Q

When viewing an object you should record what?

A

The total magnification