Microscopes Flashcards
Total magnification
The enlarged size of the object verses it’s actual size when viewed using both ocular and objective lenses
Objective lenses
The 4 different lenses used to view the object in 4 different magnifications. 40x 200x, 400x, 600x
Magnification of ocular lense
10x
Magnification of scanning power lens
4x
Magnification of low power lens
20x
Magnification of medium power lens
40x
Magnification of high power lens
60x
Scanning power total magnification
40x
Low power total magnification
200x
Medium power total magnification
400x
High power total magnification
600x
The microscopes used in class are parfocal meaning…
As you change the objective lenses, the object will remain mostly in focus
Resolution
How clear an image of an object appears.
Contrast
Determining different parts of the object
Field of view
The area that can be seen with the microscope
Orientation
The image of the object is the result of mirrors, causing the image to be a mirror image of the object.
First thing you do before getting a microscope…
Clear a space in your work area
When carrying a microscope…
Always use two hands. Support the arm with one hand, and the base with the other hand
What is the only acceptable thing to clean a lens with?
Lens paper
Always start on what power when using a microscope..
Scanning power
What power should the microscope be on when you put it away?
Scanning power
Never use coarse adjustment knob when?
When using medium and high power
You may use which microscope
Only your assigned one #18
Which objective lens will give you the largest field of view?
Scanning objective lens
Which objective lens do you always start viewing an object with?
Scanning objective lens
Which objective lenses do you use the fine focus knob with?
Medium and high power objective lens. Because lenses are very close to the slides.
Revolving nosepiece
The objective lenses are attached to the nose peice. Which allows you to change the objective lens in use. Rotate the nosepiece slowly, you will hear and feel a click when it is correctly in place
Arm
The part to hold when carrying a microscope
Base
Other hand is placed under the base when carrying, this part rests on the table
Coarse adjustment focus knob
A large knob on either side of the microscope. Used to move the stage up and down, to position the object in focus. This knob should be used only when the microscope is on the scanning or low power objective lenses
Fine adjustment focus knob
The smaller knob projecting out of the coarse adjustment knob. Makes very slight changes, used to bring the image of an object into sharp focus. Used on medium and high power objective lenses
Stage
The place where the slide is placed for viewing. There is a hole in the stage; the object to be viewed should be placed directly over the hole to allow light to shine through the object.
Stage clips and knobs
The metal clips holds the slide into place, and allow the slide to be moved easily to position or reposition an object
Condenser
The condenser focuses light
Iris diaphragm
This is located immediately under the stage. By moving this lever to the right or left, you can adjust the amt. of light that passes through the specimen and therefore the contrast
Light source
Comes from the base of the microscope, shines upward to illuminate an object
Reastat
Controls light. Little wheel. Needs to be turned away (all the way down) before putting the microscope back
Total magnification is figured out how?
Total mag.= ocular mag x object. mag
Pointer
The little needle that points to structures in the magnified image.
When viewing an object you should record what?
The total magnification
