Microscope sample prep

Question 1

Explain how to do a DRY mount:

Answer 1

1. Sample placed straight onto slide + observed down microscope

E.g: Hair cells

Question 2

Explain how to do a WET mount:

Answer 2

1. Solid specimens viewed whole or cut into thin pieces (sectioning)
2. Piece placed on slide
3. Stain added + covered with colour slip

E.g: Pollen, muscle and plant tissues

Question 3

Explain how to do a SUSPENSION mount:

Answer 3

1. Specimens suspended in liquid (usually oil or water)
2. Cover slip placed over sample

E.g: Aquatic organisms

Question 4

Explain how to do a SQUASH slide:

Answer 4

1. Wet mount prepared + covered with lens tissue
2. Pressure applied to slide

• Good for absorbing soft tissues
  E.g: Root tips

Question 5

Explain how to do a SMEAR slide:

Answer 5

1. Edge of slide used to smear sample, creating thin + even coating on slide
2. Coverslip placed on sample

E.g: Blood

Question 6

Why do u add a stain?

Answer 6

• To increase visibility of specimen
• Gives contrast between specimens + background, gives contrast between cell organelles (both worth 1 mark total)