Electron microscopes Flashcards

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1
Q

What are the 2 types of electron microscopes?

A

Transmission electron microscope and scanning electron microscope

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2
Q

What is a transmission electron microscope?

A

Electrons are transmitted through specimen, can see cell internally

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3
Q

What is a scanning electron microscope?

A

electrons hit the surface of specimen then bounce off surface, gives 3D image of the surface of cell

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4
Q

What are the types of preparation?

A

fixing, staining with heavy metals, dehydration, and embedding in resin

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5
Q

Why do we use preparation?

A

so theres a hard surface for electrons to bounce off and hit

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6
Q

Why are dead specimens used?

A

since its carried out inside a vacuum, only dead specimens used due to harsher prep needed. can cause artefacts to appear

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7
Q

What are artefacts?

A

Things created on the slide when prepping (e.g. bubbles)

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8
Q

Why do we dehydrate specimens?

A

To dry out the specimen to go into the vacuum

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9
Q

Why are electron microscopes better than light microscopes?

A

A beam of electrons is used rather than light, they have a shorter wavelength = better resolution, cell organelles visible

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10
Q

Why is having higher resolution + magnification important?

A

Means you can see cells clearer + in more detail

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11
Q

How are electrons microscopes different to light microscopes?

A

Theyre more expensive, have higher resolution + magnification, and produce black + white images

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