Microscope

Question 1

Magnification:

Answer 1

makes objects look larger (unlimited)

Question 2

Resolution:

Answer 2

the ability to see two items as separate and distinct (limited)

Question 3

Resolving power:

Answer 3

measure of clarity of an object

Question 4

Refraction:

Answer 4

bending of light when it changes (air or oil)

Question 5

Transmission:

Answer 5

light rays pass through an object

Question 6

Fluorescence:

Answer 6

Use of UV light, absorbs light at one wavelength and emits light at a different wavelength instantaneously

Question 7

Absorption:

Answer 7

Light rays are taken in by an object and absorbed

Question 8

Resolving power (Resolution):

Answer 8

Photon wavelength / (2 NA)
- Numerical aperture = light gathering ability of a lens

Question 9

Light microscopy:

Answer 9

Photons scattered by objects for visualization
Subtypes: 5

Question 10

Electron microscopy:

Answer 10

Electrons scattered by objects for visualization
Subtypes: SEM and TEM

Question 11

Subtypes of Light Microscopy:

Answer 11

1. bright-field
2. phase contrast
3. differential interference contrast
4. dark-field
5. fluorescence

Question 12

Contrast:

Answer 12

differences in intensity/color against background