Microbiology (I) Flashcards
What part of the Cell wall on gram POSITIVE organisms will induce TNF and IL-1?
What part of the cell outer membrane gram NEGATIVE orgnisms induces TNF and IL-1?
Gram + cell wall makes Lipoteichoic acid–> induces IL-1 and TNF and is major surface antigen
Gram - outter membrane makes Lipid A–> inducses IL-1 and TNF and is major surface antigen
- Location of B-lactamase enZ in bacteria
- Provides rigid support and protects against osmotic gradient (has peptide side chains with x-link via transpeptidase)
- Protects organims against phagocytosis
- mediates adhereance to surfaces, especially foriegn surfaces
- Periplasm
- Peptidoglycan
- Capsule
- Glycocalyx
What is unique about Mycoplasm bacteria?
What about Mycobacteria?
Mycoplasma has sterols but no cell wall
mycobacteria have mycolic acid and high lipid content
What bugs don’t Gram Stain well?
These Microbes May Lack Real Color.
Treponema (too thin to be visualized).
Mycobacteria (high lipid content; mycolic acids in cell wall detected by carbolfuchsin in acid- fast stain).
Mycoplasma (no cell wall).
Legionella pneumophila (primarilyintracellular).
Rickettsia (intracellular parasite).
Chlamydia (intracellular parasite; lacks classic peptidoglycan because of low muramic acid).
What bugs stain with Giemsa stain?
Certain Bugs Really Try my Patience.
Chlamydia, Borrelia, Rickettsia, Trypanosomes, Plasmodium.
What Stains with Ziehl-Neelson stain?
Inian Ink?
Silver stain?
Ziehl =Acid-fast bacteria (Nocardia, Mycobacteria), protozoa (Cryptosporidium oocysts).
Indian ink = Cryptococcus Neoformans
Silver Stain= Legionella, H.pylori and Fungi (Pneumocystisis)
Cryptococcus neoformans will stain……
with India Ink
What organisms stains PAS+?
What disease pathology does it cause?
Stains glycogen, mucopolysaccharides; used to diagnose Whipple disease (Tropheryma whipplei)
Chocolate Agar with factors V (NAD+) and X (hematin)
H.influenza = gram - rod
What special culture can you use to detect N. Meningitis or N. Gonorrhea
Thayer-Martin (VPN) media: Vancomycin (inhibits gram-positive organisms), Trimethoprim, Colistin (inhibits gram- negative organisms except Neisseria), and Nystatin (inhibits fungi)
Very Typically Cultures Neisseria
What bug do we culture on a Potatoe agar = Bordet-Gengou agar
Bordetella Pertuss
Bordet = Bordetlla
What cultures on Tellurite agar or Loffler medium?
What cultures on Lowenstein Jensen agar?
What cultures on Eaton Agar but requires cholesterol?
Tellurite and Loffler = Cornye Diptheria
Lowentein-Jensen agar = M.Tuberculosis
Eaton =M.Pneumonaie
What is special about Nocardia, Pseudomonas aeruginosa and Mycobacterium TB?
All Obligate Aerobes and use an O2 dependent system to generate ATP
Nagging Pests Must Breath
(reactivated TB likes to go to Apices of lung d/t high O2 content)
P.aeruginoas is aerobe seenin burn wounds, comoplication of diabetes
What bacteria are Obligate Anaerobes?
Why are thye anaerobes?
What antibiotics are useless against them?
Anaerobes Frankly Can’t Breathe Air:
Fusobacterium, Clostridium, Bacteroides, and Actinomyces.
They l_ack catalase and/or superoxide dismutase_ and are thus susceptible to oxidative damage.
AmiO2lgycosides are worthless on these guys bc they need oxygen to enter bacterial cell
What bugs are Obligate intracellular organims?
Stay inside (cells) when it is Really CHilly and COld.
Rickettsia, CHlamydia, COxiella. Rely on host ATP.
What bugs are facultative intracellular bugs?
Some Nasty Bugs May Live FacultativeLY.
Salmonella, Neisseria, Brucella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia pestis.
What are my Encapsulated Bacteria?
What doe the capsule do for them?
What pts are at risk for infections with these?
SHiNE SKiS.
Streptococcus pneumoniae, Haemophilus influenzae type B, Neisseria meningitidis, Escherichia coli, Salmonella, Klebsiella pneumoniae, and group B Strep.
Their capsules serve as an antiphagocytic virulence factor. Capsule + protein conjugate serves as an antigen in vaccines.
Asplenics have decreased opsonizing ability and thus increased risk for severe infections. Give S. pneumoniae, H. influenzae, N. meningitidis vaccines
What are my Catalase + organisms?
What does it mean to be catalase +?
What Disease is susceptible to Catalase + organisms?
Cats Need PLACESS to hide.
Nocardia, Pseudomonas, Listeria, Aspergillus, Candida, E. coli, Staphylococci, Serratia.
Catalase degrades H2O2 into H2O and bubbles of O2 before it can be converted to microbicidal products by the enzyme myeloperoxidase. People with chronic granulomatous disease (NADPH oxidase deficiency) have recurrent infections with certain catalase ⊕ organisms.
What organsims are Urease +?
CHuck Norris hates PUNKSS.
- *C**ryptococcus, H. pylori, Proteus, Ureaplasma, Nocardia, Klebsiella, S. epidermidis,
- *S**. saprophyticus.
- This bacteria produces yellow sulfur granules
- This bacteria produces yellow pigments
- This bacteria shows up as blue-green
- This bacteria gives off a red pigment
Actinomyces israelii= yellow sulfur
Staph. Aureus = yellow pigment
Pseudomonas Aeruginosa = blue-green pigment
Serratia Marcescens = red pigment