Microbiology Cameron McCloskey Flashcards

1
Q

Gram+ organisms stain…

A

Purple
Stain well due to thick peptidoglycan layer

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2
Q

Gram- organisms stain…

A

Pink
Thinner peptidoglycan layer doesn’t retain stain well

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3
Q

Endotoxin is produced by…

A

Grams -

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4
Q

Exotoxin is produced by…
A

A

Gram+ organisms and G-
Exported from the cell

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5
Q

Gram+ cocci in chains are?
A

A

Streptococciستريبتو كوسكاي

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6
Q

Gram+ cocci in clusters are?
A

A

Staphylococci ستافيلو كوكاي
Coagulate positive in clusters

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7
Q

The test to classify different Streptococci spp.? ستريبتو كوكاي

A

Haemolysis
Alpha (partial) - Strep. pneumoniae + viridans
Beta (complete) - Group A (pyogenes) and B Strep.
Gamma (none) - Enterococcus sp.

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8
Q

Examples of: Alpha-haemolytic Streptococci?
A

A

Strep. pneumoniae نومونيا + “viridans” group

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9
Q

Examples of: Non-haemolytic Streptococci?
A

A

Enterococcus sp. انترو كاكس

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10
Q

The test to distinguish Staphylococcus aureus from other Staph. spp.?
A

A

Coagulase test
+ve = Staph. aureus اوريس
-ve = other Staph. spp. and epidermidis (common skin commensals)

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11
Q

Opportunistic pathogen? ابرو تونيستك

A

Probably only cause infection in immunocompromised individuals

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12
Q

Virulence?
A

A

Degree of pathogenicity of an organism

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13
Q

Stages of viral replication?

A

-Attachment
-Entry - endocytosis
-Uncoating - viral nucleic acid released from capsid
-Nucleic acid and protein synthesis - host ribosomes used or polymerases
-Assembly = nucleic acid and proteins packed together
-Release =
Budding- Viruses released with envelope
derived from host cell membrane
Lysis- Virus accumulates until cell bursts,
kills cell

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14
Q

Virus detection?

A

PCR
Antigen detection

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15
Q

What do cytoxic T lymphocytes do in virus immunity?
A

A

Recognise proteins on cell surfaces as foreign and signals infected cell to commit suicide in order to prevent formation of new viruses

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16
Q

The 5 methods of spread of infection are…
A

A

Inhalation, Ingestion, Inoculation, mother to Infant, Intercourse

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17
Q

Viruses contain RNA or DNA, never both. True/False?

A

True
Size of their capsid (protein coat) limits space

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18
Q

Of both gram positive and negative which of the two has a larger periplasmic space?

A

A

Gram negative

(They have two different periplasmic spaces)

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19
Q

Which bacteria class has two membranes?

A

A

Gram negative

(Outer and plasma)

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20
Q

What exists on the outside of gram negative bacteria that confer antigenic properties?

A

A

Lipid polysaccharides (endotoxins)

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21
Q

Besides having antigenic properties, what other important characteristic do lipid polysaccharides possess?

A

A

They have bacterial toxin properties which cause damage to neighboring cells and evoke an inflammatory response

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22
Q

Why are gram positive bacteria more susceptible to antibiotics?

A

A

The outside peptidoglycan layer can act as a target for antibiotics such as penicillin

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23
Q

What happens when antibiotics bind to the peptidoglyan layer of gam positive bacteria?

A

A

The peptide cross links within peptidoglycan are broken down

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24
Q

What are flagella and fimbriae (pili)?

A

A

Extracellular proteinaceous extensions

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25
What 5 key factors so bacteria require to grow and thrive? A
Food (C, O, H, N, salts and vitamins etc.) Correct temperature pH Osmotic pressure Oxygen content
26
What are the stages in the chain of infection? A
1. Infectious microbe 2.Reservoir 3. Portal of exit 4. Mode of transmission 5. Portal of entry 6. Susceptible host
27
What are the only 5 ways in which infection can be spread? A
Inhalation Ingestion Inoculation (vaccination or artifical introduction) - direct or indirect Mother to infant - vertical transmission Intercourse - STI or STD
28
In what 5 situations (clinically) is hand washing necessary?
Before patient contact Before aseptic task After bodily fluid exposure After patient contact After contact with patient surroundings - when leaving zone
29
What are the three bacterial shapes? A
Bacilli Cocci Spiral shaped
30
In what two ways can cocci divide? A
In two planes - produce chains In three planes - produce clusters
31
what is Atypical Bacteria A
atypical bacteria is that they cannot be cultured in the normal way or detected using a gram stain. most often implicate in pneumonia
32
What bacterium causes the disease cholera? A
Vibrio chlolera (Vibrio family of 34 members)
33
What are rigid spiral bacteria called?
Spirillum سباي ريليم
33
What are flexible spiral bacteria called? A
Spirochaete سبايروكيت
34
How are the flagella utilised differently between spirullum and spirochaete? A
Spirillum - tufts at each end to aid movement Spirochaete - within periplasm causing cork-screw motion - efficient at travelling through secretions
35
What are fusiform bacteria? A
Bacteria with a thin strand structure that play a part in the natural gut flora
36
Why are fusiform bacteria potentially dangerous? A
By crossing the placenta in pregancy, they can cause still births and even cancer
37
With gram positive bacteria there is only one type of membrane, what is it? A
Cytoplasmic membrane
38
What is selctive media? A
This is a growth medium that contains specific nutrients for a specific type of microbe to grow over others
39
What is haemolysis? A
Destruction of red blod cells
40
What is alpha haemolysis? A
Partial haemolysis - greening of colonies (remember greening )
41
What is beta haemolysis? A
Total haemolysis - the most aggressive
42
What is gamma haemolysis? A
No haemolysis
43
What is multi locus sequence typing (MLST)? A
This allows sequences of bacterial DNA and entire genomes to be sequenced and uploaded to a database This allows previously sequenced sections to be matched to newly scanned sequences
44
Microorganisms can be classed depending on where they originate in relation to the body. What are these classifications? A
Endogenous - originate from within the body Exogenous - originates outwith host * Endotoxin is not really a toxin its a series of components from the outer surfaces of the Gram-negative cell wall, that you think of as being the predominant material the Lipopolysaccharide or LPS. Its immunostimulatory and key factor in Gram-negative sepsi
45
What are commensal organisms? A
Organisms that are Endogenous , part of the natural flora and often form mutual relations with the host examples E.(scherichia) coil (urinary infection) , Klebsiella and proteus (wound infection) .
46
What are opportunistic organisms? A
Exogenous organisms that infect the host when there is an opportunity such as immunocompromisation
47
Aerobic gram negative cocci appear in which formation?
A Diplococci ديبلو كوكاي (two cocci)
48
What are the two (out of 10) pathogenic aerobic gram negative cocci species? A
Neisseria .(ناسيريا) meningitidis - can cause meningitis Neisseria gonorrhoea كا نا ريا - can cause gonorrhoea
49
What are coliforms كولافورم? A
Gram negative bacteria - rod shaped and non-spore forming Can grow either anerobially or aerobically - better aerobically Many part of the natural bowel flora Should not enter sterile areas as can cause disease e.g. peritonitis, UTI, biliary tract infection etc
50
What is the first line antibiotic used for any infection caused by coliforms? A
Gentamicin
51
What is it on the outside of gram negative bacteria that stimulates the immune system? A
Lipopolysaccharides (endotoxins) composed of O antigens and lipids
52
Why can it be advantageous for gram negative bacteria to stimulate the immune system? A
Vascularity, available nutrients and protein production can increase
53
Describe the mechanism of fever production which lead to sepsis A
Endotoxin interacts with macrophages to stimulate immune response and cytokine release Cytokines cause adverse effects of sepsis and travel to the hypothalamus Prostaglandin E is produced which means the body perceives itself as cold Shivering is stimulated which generates heat and rasies the body temperature
54
Describe the process of sepsis A
Small blood vessels become leaky meaning fluid is lost into tissues - due to inflammation This causes hypovolaemia Blood pressure is reduced Organ perfusion becomes inadequate and organ shutdwon may occur Blood clotting system activates leading to clot formation Clotting factors become used up leading to haemorrhage
55
When will septic shock occur? A
After cell death lipopolysaccarides are released (causing inflammation), yet this also happens with valid treatment But, due to the sudden disintegration of many cells septic shock will occur due to the huge immune response initiated
56
What is the most important group of non-haemolytic streptococci? A
Enterococci ينترو كوكاي (two type of enterococcu**s E.faecalis and E.faecium)**
57
What is Clostridium كلوست ريديم spp and why is it hard to treat? A
Gram positive anaerobic baccili باسلي - part of normal bowel flora Spores are produced - can live outside body Exotoxins produced can cause severe body damage Antibiotic resistant (spore production) and general resistance
58
What are the two main classes of antiobiotic that related to their function? A
Bacteriostatic - inhibits growth Bacteriocidal - kills bacteria
59
What are narrow spectrum antibiotics? A
Antibiotics which are very specific in the bacteria that they target
60
Which types of antibiotics will target the cell wall? A
B lactam (includes penicillins) Glycopeptide
61
Describe how penicillin halts cell wall production A
Peptide cross links exist in the cell wall These are formed from D alanine to D alanine terminal proteins Such terminal proteins are formed when transpeptidase cleaves this dipeptide, releasing energy and creating the bond Penicillin resembles a terminal protein such as this and can bind irreversible to transpeptidase which halts cell wall production (((((bactericidal antibiotic))))
62
What are beta lactam antibiotics? A
Antiobiotics containing the beta-lactam ring in their molecular structure Common in penicillins, co-amoxiclav, cephalosporins etc.
63
Name a penicillin which targets gram positive bacteria well A
Flucloxacillin فلوكلو كلاسلين (IV or oral)
64
What are the 3 forms of penicillin? A
Benzylpenicillin |(b'enzil) (penicillin G) - IV Phenoxymethyl فا نوكسي penicillin (penicillin V) -oral Benzathine بين زاثين penicillin - IM
65
What is flucloxacillin فلكو كلاسيلين commonly prescribed for?
Streptococcus aureus Skin infections from staph and strep Deals with gram positive bacteria
66
What is the purpose of clavulanic كلافالانك acid in co-amoxicillin ? A
B lactamases are microorganisms that destroy the B lactam ring of antibiotics including amoxicllin renderinging them useless Clavulanic acid inhibits B lactamases which allows the B lactam ring to remain intact. Essentially co-amoxiclav is a "work around" to allow amoxicillin to work where it wouldn't normally
67
What is special about temocillin? A
It is resistant to B lactamases It will generally target coliforms
68
Cephalosporins are very attractive antibiotics due to their bacteriostatic and bacteriocidal properties as well as being B lactamase resistant. However, what is their major disadvantage? A
They are broad spectrum so cause damage to the natural flora This clears the environment for dangerous bacteria which they cannot kill They remove the competition for harmful bacteria
69
Do glycopeptide antibiotics possess a B lactam ring in their structure? A
No
70
Give two examples of glycopeptides A
Vancomycin Teicoplanin تيكو بلينين
71
How do glycopeptides function? A
They bind to the end of peptides This prevents peptide binding proteins such as transpeptidase from binding to their substate (the protein) Proteins cannot be incorporated into the cell wall Peptidoglycan cannot be synthesised Cross links are reduced and the cell wall is weakened
72
Why can glycopeptides only function on gram postitive bacteria? A
The outer membrane in gram egative bacteria blocks any activity they could have on the peptidoglycan layer
73
Describe how antibiotics can target bacterial protein synthesis A
Human ribosomes are different to bacterial ribosomes Antibiotics are taken into bacterial cells by active transport They bind to the 30S subunt and prevent tRNA from binding at receptor sites The chain of elonagtion is inhibited
74
What type of antibiotic is gentamicin جين سيمنتن and why is it bactericidal? A
Aminoglycoside Gentamicin targets protein synthesis When it binds to the 30S subunit in the ribosome, it inhibits protein sythesis bactericidal antibiotics, meaning that they kill bacteria rather than just inhibiting their growth.
75
What is the mode of action for the tetracyclines? A
They are transported into the cell and bind to the 30S subunit of the ribosome. This blocks tRNA binding sites and stops chain elongation
76
ive an example of a tetracycline تيجري سكيلين antibiotic A
Doxycycline Tetracycline Minocycline
77
What is the mode of action for macrolides?
They are taken into cells and bind to the 50S subunit in bacterial ribosomes. Prevent peptidyl transfer in proteins during translation. (chloramphenicol has similar process) They can be lipophilic antibiotics and can easily pass through cell membrane to "find" bacteria hiding from the immune system.
78
Give an example of a macrolide antibiotic A
Erythromycin ايري ثرومايسن Clarithromycin كليري ثرومايسن Azithromycin ايزي ثرومايسن
79
Describe how quinolone كوين الون work
They bind to the alpha subunit of DNA gyrase and prevent supercoiling of DNA which indirectly inhibits DNA synthesis They are bactericidal
80
What is an example of a fluroquinolone? فلورو كوينلون
Ciprofloxacin سيبرو فلوكسيسين Levofloxacin سليفو فلوكسيسين
81
Why is the use of fluroquinolones فلورو كولو لون very restricted?
They are broad spectrum
82
Why is folic acid required by bacterial cell and which antibiotics can prevent its synthesis?
Required to syntheiss key cell components Sulphonamides and trimethoprim جراي ميث برم ، سيلفا نيماد • Both Excreted via the urine
83
What are the serious side effects of gentamicin?
Renal damage and damage to cranial nerve VIII ( vestibulocochlear nerve)
84
Which antibiotics are associated with increased risk of clostridium difficile كلوستيريم ديفيسيل C diff? Which cause bowel infection
Cephalosporins سيفرو سبرانس Co-amoxiclav كو أيموكسي كلاف Ciprofloxacin سبرو فلوكسيسين Clindamycin كلندومايسين
85
What is a biofilm?
A protective layer around a microorganism that allows it to grow in different environments
86
What are persister cells?
Cells that are extremely resisatnt to antibiotics This is because they are encased within biofilms and cannot be targeted by antibiotics when in a dormant or inactive state - which they often are
87
What are some methods that bacteria use for horizontal gene transfer?
Transformation - genetic material is released into the extracellular space Transduction - via bacteriophages (viruses that use bacteria to replicate within) Conjugation - exchange of plasmids through direct comtact via sexual pilus
88
What are the two different types, or stages, of resistance? (2)
Cross resistance - resistant to single antibiotic(s) using the same mechanism Multiple resistance - resistant to multiple unrelated antibiotics
89
What is MRSA? A
Methicillin Resistant S. aureus A "superbug" It has an altered target site, so is difficult to treat
90
Therefore, only a small list of Gram-positive rods (bacilli) need to be memorised to categorise all bacteria - mnemonic = ABCD L
Actinomyces اكتينو مايسيس Bacillus anthracis (anthrax) بسيليس انثرسيس Clostridium كلا ستري ديي ام Diphtheria: Corynebacterium diphtheriae. (dip.thee.ree.uh) Listeria monocytogenes لستيريا مانو سايتاجنيس
91
Remaining organisms are Gram-negative rods, include
Escherichia coli Haemophilus influenzae Pseudomonas aeruginosa Salmonella sp. Shigella sp. Campylobacter jejuni
92
mechanism of resistnace aganist Penicillins
Bacterial penicillinase (β-lactamase) cleaves β-lactam ring
93
mechanism of resistnace aganist Cephalosporins
Changes to penicillin-binding-proteins (PBPs), which are types of transpeptidases*
94
mechanism of resistnace aganist Macrolides
Post-transcriptional methylation of the 23S bacterial ribosomal RNA
95
mechanism of resistnace aganist Fluoroquinolones (2)
Mutations to DNA gyrase topoisomerase and efflux pumps which reduce intracellular quinolone concentration
96
mechanism of resistnace aganist Tetracyclines
Increased efflux of the bacteria by plasmid-encoded transport pumps, ribosomal protection
97
mechanism of resistnace aganist Aminoglycosides
There are three mechanisms of aminoglycoside resistance: reduced uptake or decreased cell permeability, alterations at the ribosomal binding sites, or production of aminoglycoside modifying enzymes- acetyltransferases, adenyltransferases and phosphotransferases
98
mechanism of resistnace aganist Sulfonamides
competitively inhibit the incorporation of PABA into folic acid, thereby preventing the synthesis of folic acid.
99
mechanism of resistnace aganist Vancomycin
Alteration to the terminal amino acid residues of the NAM/NAG-peptide subunits (normally D-alanyl-D-alanine) to which the antibiotic binds.
100
antibitotics Inhibit cell wall formation (BETA-lactams)?
* penicillins: binds transpeptidase blocking cross-linking of peptidoglycan cell walls * cephalosporins modified beta lactation *
101
Inhibit protein synthesis: these antibiotics are bateriostatic
* aminoglycosides (cause misreading of mRNA) * chloramphenicol * macrolides (e.g. erythromycin) * tetracyclines * fusidic acid All are bacteria which inhibit protein synthesis
102
Inhibit DNA synthesis
* quinolones (e.g. ciprofloxacin) * metronidazole * sulphonamides * trimethoprim
103
Inhibit RNA synthesis
rifampicin ريفام بسين
104
Which step cause the purple appearance of G+ in gram stain (cry Which step causes the pink appearance of G- in gram stain (saf)
Primary dye: A primary stain (crystal violet) is applied to a heat-fixed smear of bacterial culture. Trapping: Iodide is added as a mordant. This binds crystal violet within the cell wall and traps it there. Decolorisation: The smear is rapidly decolourised with ethanol or acetone. Counterstain: A second dye (safranin or carbol fuchsin) is applied to counter stain the sample.
105
+ Examples of enveloped DNA viruses + Examples of enveloped RNA viruses include + Examples of non-enveloped viruses include
+ include herpesvirus, poxvirus, and hepatitis B virus. + hepatitis C virus, coronavirus, influenza, mumps, measles, rabies, ebola, and retroviruses + rotavirus, poliovirus, norovirus and coxsackieviruses.
106
determine what each indicate ?
Positive HBsAg indicates current infection, which may be either acute or chronic IgM anti-HbcAg indicates acute infection; IgG anti-HbcAg indicates chronic or resolved infection Positive anti-HBsAg demonstrates immunity, whether from vaccination or natural infection
107
Hydrogen peroxide was added to a small sample of the culture, with no observable reaction. What organism is most likely responsible for this presentation?
All species of streptococcus are catalase negative, so will not produce gas on addition of hydrogen peroxide
108
What is the diffrent btween lipopolysaccharide and peptidoglycan ?
* lipopolysaccharide are presnet on the outer memebrane of gram negtive bacteria * peptidoglycan are presented in both G+/- but moslty in G+
109
Which antibiotics are used from gram positive cover
Glycopeptides
110
Quellung reaction (quel-lung)
is the only test which may be used to identify Streptococcus pneumoniae,
111
what is ASO titre
Antibody titre to Streptolysin O (ASO titre) used to determine recent infection with Streptococcus pyogenes
112
what does Viridans group Streptococcus (no Lancefield classification) cause
dental infections,** endocarditis**, abscesses
113
All species of streptococcus are catalase negative True/Flase
True
114
Penciline allergy would lead to the symptons of
anapylaxis * symptoms include unable to speak and starts to gasp for air
115
main sid effect of aminoglycoside are
nephrotoxicity and ototoxicity
116
untreated sort throat infection can lead to
* Rheumatic fever: * Scarlett fever * Post streptococcal glomerulonephritis (PSGN)
117
side effect of vancomycine
Vancomycine : side effect include damages kidneys, occasionally causes ‘red man syndrome’ (allergy) include rash with more eosinophilia, and low neutropenia * Can only be given IV
118
Which of the following statements is correct regarding bacterial toxins? Endotoxins are only produced by Gram positive bacteria Excessive release of endotoxin causes toxic shock syndrome Exotoxins do not activate the host adaptive immune response Endotoxin is a major pathogen-associated molecular pattern (PAMP) which activates the host innate immune response Endotoxin is secreted by all bacterial cells
Endotoxin, or bacterial lipopolysaccharide (LPS), is a pathogen-associated molecular pattern (PAMP) recognised by toll-like receptor 4 (TLR4), part of the innate immune system. Recognition results in the toxic biological effects of endotoxin, notably local inflammation and fever. Large amounts of endotoxin can cause a massive pro-inflammatory reaction and endotoxic shock
119
Shiga Toxin producing wich exotoxin ?
You have two example of an exotoxin linked to C. difficile, that damage membrane and Shiga/Vero toxin produce strains (S/VTEC) that also damge GI tract epithelial. So what a toxin is, protein component to components that cause damage outside the bacteria in the host/environment. Endotoxin is not really a toxin its a series of components from the outer surfaces of the Gram-negative cell wall, that you think of as being the predominant material the Lipopolysaccharide or LPS. Its immunostimulatory and key factor in Gram-negative sepsis.. the condiiton you have been briefly introduced too. I would add the key learning outcome is that you can contrast the two, i.e. identify that the endotoxin is not a really a toxin.
120
In the context of antibiotic resistance what are these process (3)
1. Internal mutations e.g. point mutations, insertions, deletions, transpositons etc 2. Acquisition of foreign DNA from plasmids, transposons or bacteriophages 3. Alteration of bacterial membranes: prevents antibiotics entering bacterium
121
Efflux Pumps
are transport proteins involved in the extrusion of toxic substrates (including virtually all classes of clinically relevant antibiotics) from within cells into the external environment. Commonly occurs against tetracyclines and fluoroquinolones, macrolides.
122
Mehtods of antibotic resistance with exmaples (4)
Efflux Pumps e,g tetracyclines and fluoroquinolones, macrolides. Alterations of ribosomal target sites. antiribosomal agents e.g. tetrayclines, macrolides, clindamycin, aminoglycosides Alterations of cell wall precursor targets. glycopeptide antibiotcs e.g. vancomycin, enterococci/staph aureus. Alterations of critical enzymes . Alterations of critical enzymes. e,g, alteration of penicillin binding proteins prevents binding of beta-lactam antibiotics, epseically in streptococci and staph aureus.
123
Penicillin is only used for ________
Gram Positive bacteria
124
Flucloxacillin is usually prescribed for
prescribed for staph and strep skin infections
125
Glycopeptide antibiotics work by inhabiting protein synthesis True / False
False they Interference with cell wall synthesis
126
family members' of : Aminoglycosides Quinolones (fluoroquinolones) Macrolides Cephalosporins
Gentomycine ciprofloxacin and levofloxacin erthromycin , azithromycin and clarithromycin Cefaclor, Cefadroxil and cefalexin and ceftriaxone Note that all antiribosmes end with cin however vancomycin is interfere with amino acid of cell wall and Tetracyclines are also antribosmes but don't end with cin
127
A Patient has a penicillin allergy, what is likely to be prescribed instead?
Tetracyclines (e.g. doxycycline), quinolones (e.g. ciprofloxacin), aminoglycosides (e.g. gentamicin) and glycopeptides (e.g. vancomycin) are all unrelated to penicillins and are safe to use in the penicillin allergic patient. Especially macrolides (e.g. clarithromycin),
128
A. Mycobacterium tuberculosis stain well with gram staining true/false
FLASE
129
Obligate anaerobes
can servive in any oxygenated (aerobic) or no oxygen (anaerobic) environment
130
Name the test used to distinguish Staphylococcus species. Streptococci, enterococci Gram negative bacteria
coagulative test Haemolysis test Lactose for road shape bacteria
131
fungal infection in the oral cavity is mostly caused by
Candida fungal
132
- Gram negative cocci that appear in pairs on gram film
Neisseria spp.
133
- Staphylococcus causes scarlet fever True /False
FALSE its Streptococcus spp.
134
Syncitia formation in viruses
Some viruses involve in transformation of host cell into begging or malignant
135
Viral persistence
they may become quiescent (no active replication)
136
Immune reactivity to viruses include
CD8+ lead to apoposis of infected cell Neutralisation by IgM and IgG which used in antibody detection methods
137
Immune reactivity to viruses include
CD8+ lead to apoposis of infected cell Neutralisation by IgM and IgG which used in antibody detection methods
138
What does MRSA stand for?
Methicillin-resistant staphylococcus aureus
139
Which endotoxin in gram negative bacteria can trigger Acute Respiratory Distress Syndrome (ARDS?
lipopolysaccharide (LPS) also known as endotoxin. When activatedneutrophils release various substances including enzymes and reactive oxygen species that can damage nearby cells and tissues. enter the lungs and release inflammatory mediators. This is termed Acute Respiratory Distress Syndrome (ARDS), widespread inflammation of the lungs resulting in acute breathlessness that can be fatal.
140
What kind of bacteria is streptococcus pneumonia (Gram, haemolytic, Group)? Streptococcus pneumoniae is an alpha-haemolytic gram-positive bacterium. It has no Lancefield grouping. What are the immunogenic complications of Group A streptococcus? (3)
Rheumatic fever Rheumatic heart disease Acute glomerulonephritis
141
One difference between Pilus and Fimbrae
Involve in bacterial conjugation Act as adherent attachment for different type of cell surface
142
What is common about clostridium , bacillus anthracis
Produce endospores
143
What is the difference between capsule and slim layer cell envelops
Capsule made of organized polysaccharides , has virulence factor e.g Strep . Pneumonia Slim layer made of unorganized polysaccharides allow cell adherent e.g pseudomonas.
144
Which part of the L.PS in G- which cause inflammation ? And which part of the bacteria do antibodies bind to ?
Lipid A O-antigen
145
Where are beta-lactamases found in a bacterial cell?
In the extra cellular space called periplasmic space
146
Different between G+/-
147
4 steps of Gram Staging
1. Crystal violet 2. Apply mordant Iodine 3. Apply ethanol 4. Apply counter stain Safranin
148
These Atypical Microbes usually Lack Colour Because Microbes Barely Eat Ramen (these are G- bacteria)
Trypinema , Anaplasmosis , Mycoplasma Ureaplasma , Leptospira, Chlamdiea , Bartonela , Mycobacteria , Borrelia , Elichia , Ricketsia
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a component of the strain of MRSA (Methicillin Resistant Staphylococcus Aureus) which causes severe skin infection such as necrotising fasciitis?
Panton Valentine Leukocidin is a cytotoxin associated with highly virulent strains of Staph. aureus causing necrolytic skin infection.
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The microbiology film shows circular organisms arranged into lines. Which genus does this organism belong to?
(Streptococci), strep = strips/lines, cocci = circular
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This organism produces a golden culture on a blood agar plate. Which organism is this?
(Staph. Aureus), staph aureus classically shows up golden on a blood agar plate. It is the only organism that does this.
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virus can stay in the body in a latent state and reactivate to cause disease?
(Herpes Simplex Virus) – Latency is characteristic of a herpetic viral infection. HSV is responsible for recurrent cold sores and genital herpes.
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bacteria which is resistant to most oral antibiotics
Pseudomonas aerugniosa is a gram-necative coccabicallus, which is resistant to most oral antibioticcs.
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****Coagulase and catalase positive cocci with a gold aura”
Staphylococcus aureus
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Describe the appearance of neisseria meningitidis
**N. Meningitidis is an aerobic gram negative coccus which appears as diplococci. ** stain pink or red on a Gram stain.
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Staphilococci?
Staphylococci are Gram positive cocci that appear round in grape-like clusters under the microscope
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The circulating blood volume is decreased in the septic patient Sepsis is easily treated with antibiotics True / false
In sepsis there is a decrease in circulating blood volume due to endotoxins causing vasodilation & intravascular fluid to leak into adjacent tissue.
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difference between Streptococcus? and Streptococci & Staphylococci
Streptococcus appears as Gram-positive cocci in chains on Gram stain. Streptococci and Staphylococci are both Gram-positive cocci, which means that they appear as spherical-shaped bacteria that stain purple on a Gram stain. Streptococci are often arranged in chains and can be further classified based on their hemolytic properties on blood agar. Staphylococci, on the other hand, are often arranged in clusters and can be differentiated from Streptococci by their ability to form clusters and by their coagulase activity.
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Which cellular component is targeted by beta-lactam antibiotics?
Cell wall
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macrolides are excreted by urine true/ false
false the excreted by liver they are lipophlic
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what is the difference between G+ and G- cell walls
G+ have thick cell wall with no extra cover G- thin cell wall with outer membrane cover
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Gram-Positive Rods “corney Mike’s list of basic cars”:
- Corney – ***Corneybacteria*** - Mike’s – ***Mycobacteria*** - List of – ***Listeria*** - Basic – ***Bacillus*** - Cars – ***Nocardia***
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Gram-Positive Cocci
- Staphylococcus - Streptococcus - Enterococcus
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Gram Positive Anaerobes | “CLAP”
- **C** – **C**lostridium - **L** – **L**actobacillus - **A** – **A**ctinomyces - **P** – **P**ropionibacterium
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Atypical Bacteria | legions of psittaci MCQs”:
- Legions – ***Legionella pneumophila*** - Psittaci – ***Chlamydia psittaci*** - M – ***Mycoplasma pneumoniae*** - C – ***Chlamydydophila pneumoniae*** - Qs – ***Q fever*** (***coxiella burneti***)
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When learning antibiotic coverage I used this stepwise process of escalating antibiotic treatment in a hospital patient to remember what antibiotics covered what bacteria
- Start with ***amoxicillin*** which covers ***streptococcus***, ***listeria*** and ***enterococcus*** - Switch to ***co-amoxiclav*** to additionally cover ***staphylococcus***, ***haemophilus*** and ***e. coli*** - Switch to ***tazocin*** to additionally cover ***pseudomonas*** - Switch to ***meropenem*** to additionally cover ***ESBLs*** - Add ***teicoplanin*** or ***vancomycin*** to cover ***MRSA*** - Add ***clarithromycin*** or ***doxycycline*** to cover ***atypical bacteria***
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* Viral persistence
- refers to the ability of certain viruses to remain in the body for extended periods of time, either by - becoming quiescent (no active replication) - or by causing chronic infections.
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When you can prescribe antibodies
Dermatology * Trauma, Folliculitis, abscess, cellulitis Urinary Tract Infections * Catheter * Age * Pain, blood, cloudy, protein Respiratory Tract Infections * Sore throat * Otitis media * Sinusitis * Pharyngitis Urgent Scenarios include; * Sepsis * Cellulitis * Meningitis * Infective Endocarditis
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Mecahnism of action of pencillin
Beta-lactam ring to inactivate cell wall transpeptidases?
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, appear as Gram positive spherical cells arranged in grape like clusters.
D. Staphylococcus
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yellow urethral is due to what type of route of infection
sexual intercourse
172
A 30 year old male has jaundice and deranged liver function tests 3 months after a holiday overseas. He admits to getting a new tattoo on holiday, in an unlicensed tattoo parlour for a dare
This is most likely to be hepatitis B, spread by blood to blood contact on improperly sterilised equipment, the incubation period is 6 weeks to 6 months and about half of infected adults have jaundice.
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flagella' on the bacteria is used for
B. Protein molecules that extend from the surface of bacteria & generate movement
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microbiome'
Th genetic material associated with all the micro-organisms colonising the body
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Malaria is caused by
a protozoa of the genus plasmodium
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A. A prokaryote that contains a single cytoplasmic membrane surrounded by a relatively thick multi-layered cell wall that often contains a secondary polymer in addition to peptidoglycan. this describe a gram
G+
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antibiotic that is taken up in an inactive form and reduced in the cytoplasm of the micro-organism leading to DNA damage.
D. Metronidazole (Nitroimidazole)
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In which scenario would 'transmission based' rather than 'standard' infection control be appropriate? A. Attending a patient consultation for an individual who has tested positive for Escherichia coli bacteremia B. Attending a patient consultation for an individual who has tested positive for Staphylococcus areus bacteremia C. Attending a ward where patients suffering with C. difficile infections have been identified D. Receiving an individual who has returned from holiday showing symptoms of Middle-Eastern Respiratory Syndrome (Coronavirus) E. When dealing with a catheterised patient in an ICU ward
Correct Answer(s): C Justification for correct answer(s): Transmission-Based Precautions are the second tier of basic infection control and are to be used in addition to ‘Standard Precautions’ for patients who may be infected or colonized with certain infectious agents for which additional precautions are needed to prevent infection transmission.
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Which virus is commonly associated with malignant epithelial tumours in humans?
Human papilloma virus
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macrophages relase
Pyrogen = induces fever