Histology Cameron McCloskey Flashcards
The plasmalemma is known by which other name?
Cell membrane
Many proteins are anchored into the cell membrane, what functions may they have?
- Receptors
- Enzymes
- Channels
- Transporters
What are the three types of filaments that make up the cytoskeleton of a cell?
- Microfilaments
- Intermediate filaments
- Microtubules
What are the smallest type of filaments that make up the cytoskeleton?
Microfilaments
(approx 7nm diameter)
Microfilaments are composed of what?
Actin
There are more than 50 classes of intermediate filaments, what can each class give an indication of?
Origin of the cell in the body
(this can help when locating tumours)
What are microtubules composed of?
Alpha and beta tubulin subunits
Which two proteins can use microtubules as a transport network and which direction does each travel?
- Kinesin - An ATPase that transports compounds towards the cell periphery
- Dynein - An ATPase that transports compounds towards the cell centre
The nucleus is surrounded by an _______ and ________ membrane
Inner and outer
The outer membrane of the nucleus shares a common border with what?
The rough endoplasmic reticulum
What is between the inner and outer nuclear membranes?
Perinuclear cisterna
The space between the inner and outer nuclear membranes is also called the perinuclear space. The perinuclear space (PNS) is joined with the lumen of the rough endoplasmic reticulum (RER)
There are three types of RNA, which two are transcribed and which is transcribed in the nucleolus
Nucleus - mRNA, tRNA
Nucleolus - rRNA
The nucleus contains two states of DNA, what are these states?
- Heterochromatin - DNA condensed and not undergoing transcription
- Euchromatin - DNA untwisted and undergoing transcription
Where are ribosomes formed?
Nucleolus
What two subunits make up a ribosome?
- Small subunit - binds to mRNA
- Large subunit - catalyses formation of peptide bonds
What are the two types of endoplasmic reticulum?
- Rough
- Smooth
What are polysomes?
Clusters of free ribosomes in the cytoplasm surrounding mRNA which is being transcribed
What is the role of the smooth endoplasmic reticulum?
Processing proteins after passage through the rough endoplasmic reticulum and also for the synthesis of lipids
What is the role of the rough endoplasmic reticulum?
The synthesis of membrane bound proteins or secretions.
It is also the site of glycoprotein formation
What is the role of the golgi apparatus كولجي ا برادس?
The pakaging and processing of proteins for export to their destinations
The golgi apparatus is made up of many flattened sacs resembling plates, what is each sac called?
A Cisterna
How do proteins arrive at the golgi apparatus?
In transport vesicles from the smooth endoplasmic reticulum
What is the inner membrane of mitochondria called?
The cristae
This is folded to increase surface area
DNA can be found in which two locations within a cell?
- Nucleus
- Mitochondria - they have their own DNA
Which type of muscle will have many mitochondria and which type will have few?
- Many mitochondria - Slow twitch muscle - generally aerobic
- Few mitochondria - fast twitch muscle - generally anaerobic
What are intercellular junctions?
Structures specilaised to join cells into groups to form a functional unit
Where are intercellular junctions most prominent?
Epithelial cells
What are the three types of intercellular junctions?
- Occluding اوكلودين(tight junctions) mostly at the apical area
- Anchoring
- Communicating
Occluding junctions can have which other name?
Tight junctions
(or zonula occludens)
What do occluding junctions function to do?
Link cells together to form a diffusion barrier
What are the three types of anchoring intercellular junctions? AND WHAT IS FUNCTION OF EACH ?
- Adherent : link submembrane actin bundles of adjacent cells via cadherin molecules
- Desmosomes: link submembrane intermediate filaments of adjacent cells l
- Hemidesmosomes: link submembrane intermediate filaments of cells to extacellular matrix through transmembrane proteins
Describe the function of anchoring adherent intercellular junctions
Actin in the cytoplasm is used to link adjacent cells together
Actin is bound to cadherin - a transmembrane protein which acts as an adhesive linking cells together
Describe the function of anchoring desmosome intercellular junctions
Intermediate filaments are used to join cells as they can bind with transmembrane proteins.
This can provide stablity in the skin
Describe the function of anchoring hemidesmosome intercellular junctions
link submembrane intermediate filaments of cells to extacellular matrix through transmembrane proteins
What is a junctional complex?
This is when many types of junctions are used in close proximity
This is common in epithelial cells
Communicating junctions have what other name?
Gap junctions
What are communicating junctions composed of?
A circular patch with many pores created by connexin proteins which make direct channels between cells
Communicating junctions allow for what between connected cells?
Selective diffusion and transfer of certain molecules
Communicating junctions are common in which tissue types?
- Smooth muscle
- Cardiac muscle (spread of excitation)
- Epithelial cells
There are 3 main ways in which substances can be transported across a membrane, what are these?
- Diffusion
- Protein pumps/channels
- Endo/exocytosis using vesicles (vesicular transport)
During endocytosis, material in the ___________ space can be engulfed by the cell forming an __________
Extracellular
Endosome
Often receptor mediated
What is it called when a phagosome and lysome fuse?
A phagolysosome
When tissue is prepared for microscopy which chemical may be used to cross link proteins and maintain structure?
Formalin
What is added to tissues to give it structure before it is used for microscopy?
Wax
What is a common dye used to allow differentiation in different cell parts?
“H & E”
- Haematoxylin (H) - a basic dye which binds to acidic molecules such as DNA or ribsomes causing a purple colour
- Eosin (E) - an acidic dye which binds to basic molecules such as cytoplasmic proteins causing a pinkish colour
What are the four basic tissue types?
- Epithelium
- Connective tissue
- Muscle
- Nervous
What is the basal lamina?
The extracellular matrix of components at the basal (underside) surface of epithelial cells.
Which type of cells are not vascularised and how do they aquire nutrients?
Epithelial cells
They acquire nutrients via diffusion from the basal lamina
What are the different epithelial cell shapes?
- Squamous (flat)
- Cuboidal (cube shaped)
- Columnar (cuboidal - tall and thin)
What are the names of epithelia based on the number of layers?
- One - simple
- Two or more - stratified
- Multiple layers - pseudostratified (all cells in contact with the basal lamina)
What are the two types of epithelial gland?
- Endocrine (ductless) - product secreted towards the basal end of the epithelium and distributed into the vascular system - lots of capillaries will surround the glands
- Exocrine (ducted) - product secreted upwards towards the apical end of an epithelial cell
What are the three subtypes of connective tissue?
- Soft connective tissue
- Hard connective tissue
- Blood and lymph
What are fibroblasts?
Cells that are widely distributed in the extracellular matrix helping to maitain it
What are chrondrocytes?
cells responsible for cartilage formation,
Within the extracellular matrix there is ground substance, what is this?
A large space containing a gel-like substance made of huge polysaccharide molecules called glycosaminoglycan which are mostly bound to protein cores to form glycoproteins
What are the two types of soft connective tissue?
- Dense regular - densely pack collagen fibres in ordered alignment
- Dense irregular - densely packed collagen fibres running in many directions which resists breakage
What allows cartilage to be flexible?
The ground substance in cartilage is very hydrated allowing for this type of flexible stucture
How does cartilage receive nutrients since it is not vascularised?
Diffusion through its matrix
What are the three types of cartilage?
- Hyaline
- Elastic
- Fibrocartilage
What are the two types of bone which are distinct to the naked eye?
- Cortical bone - outer shell composing the shaft (diaphysis)
- Cancellous (trabecular) bone - inside of bone, an interconnected network of bone fragments that contains small holes and hollow sections
What are osteocytes?
Bone cells
What are the three major types of muscle tissues?
- Smooth (involuntary)
- Skeletal (voluntary)
- Cardiac