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CLASP: Sepsis > Microbiology > Flashcards

Microbiology Flashcards

1
Q

define colonisation

A

presence of a microbe in the human without an inflammatory response

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2
Q

define infection

A

inflammation due to a microbe

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3
Q

define bacteraemia

A

presence of viable bacteria in blood

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4
Q

define sepsis

A

dysregulated host response to infection

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5
Q

define contamination

A

introduction of microorganism into a sample which are not disease-causing or present in the body site (may be flora or environmental)

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6
Q

diagnosing techniques for infection

A 
microbiology
microscopy
serology
antigen detection
PCR/ molecular studies
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7
Q

microbiology testing samples

A 
blood
stool
urine
wound
tissue culture
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8
Q

samples for microscopy

A

stool
urine
CSF
sputum

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9
Q

what is MALDI-TOF

A

identifies bacteria on a positive culture using mass spectrometry

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10
Q

methods for identifying bacteria

A 
macroscopic
microscopic
respiration
reproduction (spores/ no spores)
biochemistry
molecular methods
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11
Q

macroscopic methods for identifying bacteria

A 
colony
size
shape
pattern
haemolysis
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12
Q

microscopic methods for identifying bacteria

A

gram stain
organism shape
antigenic properties

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13
Q

what is coagulase +ve?

A

staph aureus

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14
Q

what is coagulase -ve?

A

staph epidermidis

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15
Q

when should you never do a urinalysis?

A

catheter

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16
Q

what is the pre-analytical phase?

A

selection of tests and collection of specimens

17
Q

what is the analytical phase?

A

testing of sample

18
Q

what is the post-analytical phase?

A

communication of result

19
Q

what is pre-test probability?

A

likelihood the patient has the diagnosis prior to the test

20
Q

what is post-test probability?

A

likelihood of patient having a diagnosis once the result is available

21
Q

what is sensitivity?

A

probability a test is positive if disease is present

22
Q

what is specificity?

A

probability a test is negative if the disease is absent

23
Q

sensitivity tests for antibiotics

A

disc diffusion
E-test
vitek

24
Q

what to be aware of with commensals?

A

unlikely to cause infection so think about result in context with patient

CLASP: Sepsis (8 decks)

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