Microbiology Flashcards
Candida
Oval, gram + structure in mouth
Diptheria
Leffler staining / Neisser medium / Blood tellurite agar
Becomes toxic due to phage conversion (produces enterotoxins)
Greyish membrane / gram + bacilli
Precipitaion line/ gel reaction test
Vaccine = antidiptheric antitoxic serum
Staphylococcus aureus
Clusters of cocci = coccus
Yellow/gold colonies = aureus
Pseudomonas aeruginosa
Produces yellow/green pigment
Cholera
Comma shaped (curved)
Gram -ve
Rice water stool
Previous Hep B infection
Anti HBs/HBc/HBb antibodies
Hepatitis
ELISA test used to confirm diagnosis
Dysentry
Causes haemagglutination
Diagnosed using bacteriological test
HIV
gp 120 binds to CD4 receptor on target cells
ELISA used to confirm diagnosis
Variola/smallpox
Paschen bodies
Romanovsky Giemsa Method
Leptospirosis = S/C shaped
Syphillis = Spiral coils
Toxoplasmosis (parasite)
Calculating coli index and titer of water
Index = no of red colonies x 2 Titer = vol of H2O divided by no of colonies
Botulism
Diagnosed using bacteriological test
Detected using precipitation neutralization test
Comes from homemade fish, meat or mushrooms
If bitten by unknown animal
Always provide anti rabies vaccine
Macrophage defect
Dysfunction of antigen presentation to immune competent cells
Salmonella
Achromic colony on endo agar
Mantoux test
Injects tuberculin to test for TB
Neg result = prescribe BCG vaccination (no immunity against TB)
Pos result = papule formation (Type IV hypersensitivity reaction)
Neisseria gonnorhea
Gram negative
Coffee bean shaped (or bin shaped)
Confirm diagnosis using microscope
Favus fungus
Causes air bubbles and fate drops in hair
Ziel - Neelsen stain
Micro bacterium TB
Red = acid resistant
Blue = alkaline resistant
Streptococcus
Long chains
Elongated
Can cause kidney damage and tonsillitis
Agglutination reaction
Adhesion of microorganisms
E. coli
Red colonies in endo agar
Causes coli enteritis
H. pylori
Microeaerophillic ability
Diagnostic titer
1:() 100 = has the disease
Widals Test
Typhoid fever
T lymphocytes
Cells that form rosettes with erythrocytes
Wound infection with gangrene
Use bacteriological test to confirm
Immunofluroessence
Used for “quick”/”immediate” testing
Reiters fixation test
Tests for toxoplasmosis and gonorrhea
Streptococci angina
Antibodies for O sterptolysin present in case of infection
Hemadsorption reaction
Detects viruses containin hemagglutinations
Enteric fever
Use blood culture to confirm diagnosis
Clostridia
Violet blue gram stains
Helicobacteria pylori
Gram negative
Spiral shaped
Positive urease test
“Hanging drop”
Phase contrast microscopy
What vaccine method provides the longest immunity
Oral vaccination with live vaccine
Rabies
Negri bodies present
Bordet Gengou test
Bordetella pertusis
CFT (Reiters Test)
Gonnorrhea and syphillis
Immunfluroessence test
For influenza
Phagetyping
To know source of infection
1:20/40/80
1: 20: To repeat test after 10 days
1: 40: Confirms diagnosis
1: 80: Carrier of disease
Cytomegalovirus
Owls eye
Coxsackie A
Mouse sucklings die
Streptococcus mutans
Enamel demineralization
