Microbiology Flashcards

1
Q

Candida

A

Oval, gram + structure in mouth

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2
Q

Diptheria

A

Leffler staining / Neisser medium / Blood tellurite agar
Becomes toxic due to phage conversion (produces enterotoxins)
Greyish membrane / gram + bacilli
Precipitaion line/ gel reaction test
Vaccine = antidiptheric antitoxic serum

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3
Q

Staphylococcus aureus

A

Clusters of cocci = coccus

Yellow/gold colonies = aureus

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4
Q

Pseudomonas aeruginosa

A

Produces yellow/green pigment

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5
Q

Cholera

A

Comma shaped (curved)
Gram -ve
Rice water stool

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6
Q

Previous Hep B infection

A

Anti HBs/HBc/HBb antibodies

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7
Q

Hepatitis

A

ELISA test used to confirm diagnosis

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8
Q

Dysentry

A

Causes haemagglutination

Diagnosed using bacteriological test

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9
Q

HIV

A

gp 120 binds to CD4 receptor on target cells

ELISA used to confirm diagnosis

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10
Q

Variola/smallpox

A

Paschen bodies

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11
Q

Romanovsky Giemsa Method

A

Leptospirosis = S/C shaped
Syphillis = Spiral coils
Toxoplasmosis (parasite)

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12
Q

Calculating coli index and titer of water

A
Index = no of red colonies x 2 
Titer = vol of H2O divided by no of colonies
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13
Q

Botulism

A

Diagnosed using bacteriological test
Detected using precipitation neutralization test
Comes from homemade fish, meat or mushrooms

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14
Q

If bitten by unknown animal

A

Always provide anti rabies vaccine

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15
Q

Macrophage defect

A

Dysfunction of antigen presentation to immune competent cells

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16
Q

Salmonella

A

Achromic colony on endo agar

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17
Q

Mantoux test

A

Injects tuberculin to test for TB
Neg result = prescribe BCG vaccination (no immunity against TB)
Pos result = papule formation (Type IV hypersensitivity reaction)

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18
Q

Neisseria gonnorhea

A

Gram negative
Coffee bean shaped (or bin shaped)
Confirm diagnosis using microscope

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19
Q

Favus fungus

A

Causes air bubbles and fate drops in hair

20
Q

Ziel - Neelsen stain

A

Micro bacterium TB
Red = acid resistant
Blue = alkaline resistant

21
Q

Streptococcus

A

Long chains
Elongated
Can cause kidney damage and tonsillitis

22
Q

Agglutination reaction

A

Adhesion of microorganisms

23
Q

E. coli

A

Red colonies in endo agar

Causes coli enteritis

24
Q

H. pylori

A

Microeaerophillic ability

25
Q

Diagnostic titer

A

1:() 100 = has the disease

26
Q

Widals Test

A

Typhoid fever

27
Q

T lymphocytes

A

Cells that form rosettes with erythrocytes

28
Q

Wound infection with gangrene

A

Use bacteriological test to confirm

29
Q

Immunofluroessence

A

Used for “quick”/”immediate” testing

30
Q

Reiters fixation test

A

Tests for toxoplasmosis and gonorrhea

31
Q

Streptococci angina

A

Antibodies for O sterptolysin present in case of infection

32
Q

Hemadsorption reaction

A

Detects viruses containin hemagglutinations

33
Q

Enteric fever

A

Use blood culture to confirm diagnosis

34
Q

Clostridia

A

Violet blue gram stains

35
Q

Helicobacteria pylori

A

Gram negative
Spiral shaped
Positive urease test

36
Q

“Hanging drop”

A

Phase contrast microscopy

37
Q

What vaccine method provides the longest immunity

A

Oral vaccination with live vaccine

38
Q

Rabies

A

Negri bodies present

39
Q

Bordet Gengou test

A

Bordetella pertusis

40
Q

CFT (Reiters Test)

A

Gonnorrhea and syphillis

41
Q

Immunfluroessence test

A

For influenza

42
Q

Phagetyping

A

To know source of infection

43
Q

1:20/40/80

A

1: 20: To repeat test after 10 days
1: 40: Confirms diagnosis
1: 80: Carrier of disease

44
Q

Cytomegalovirus

A

Owls eye

45
Q

Coxsackie A

A

Mouse sucklings die

46
Q

Streptococcus mutans

A

Enamel demineralization