Microbiology Flashcards
Explain the characteristics of a eukaryote?
- complex
- multi-cellular
- linear chromosomes
- no cell wall
Explain the characteristics of a prokaryote?
- simple
- circular plasmids
- rigid cell wall
Gram +ve cell wall is usually_________-
- thicker Peptidoglycon
- 1 layer of phopholipids
Gram -ve cell wall is usually___________
- thinner Peptidoglycon
- 2 layers of phospholipids
What colour does gram positive stain
- purple
What colour does gram negative stain?
- pink
Gram positive usually have pilus or fimbriae?
- fimbriae
Gram negative usually have pilus or fimbriae?
- pilus
What is the role of fimbriae and pilus
- fimbriae increases surface area
- adherence and sex
How do prokaryotes grow?
- by binary fission
What are some factors that effect the rate of growth in prokaryotes?
- food
- temperature
- oxygen concentration
- pH
What are the four stages of a prokaryotic life?
- lag
- log
- stationary
- decline
Exotoxins are usually produced by gram___ bacteria?
- positive
Endotoxins are usually produced by gram___ bacteria?
- negative
Shape of cocci?
- spheres
- clusters or chains
Shape of bacilli?
- rods
What are spores?
- inert structures
- resistant to physical and chemical challenge
What is an example of an organism that doesnt stain with gram staining?
- TB
Define aerobic?
- grows in oxygen
Define obligate aerobes
- requires oxygen
Define obligate anerobes
- killed by oxygen
Define facultative anerobes
- tolerated oxygen
What is a selective media?
- a media that selects for the growth of one organism over another
What is a differential media?
- incorporation of different chemicals to produce visible changes
- allows identification
How can you test streptococcus?
- haemolysis test
Alpha haemolysis looks like what? and suggests what?
- partial haemolysis
- strep pneumonia
- strep virdians
Beta haemolysis looks like what? and suggests what?
- complete haemolysis
- strep A or B