Microbiology Flashcards
chocolate agar with factors V and X
haemophilis influenza
thayer-Martin media
Neisseria gonorrhoeae and Neisseria meningitidis
VPN media = vancomycin, polymyxin, nystatin,
Bordet-Gengou (potato) agar
bordetella pertussis
Tellurit plate
Loffler’s media
C. diphtheriae
Lowenstein-Jensen agar
mycobacterium tuberculosis
Eaton’s agar
Mycoplasma pneumoniae
MacConkey’s agar
Lactose fermenting enterics = E.coli, citrobacter, klebsiella, enterobacter, serratia
eosin methylene blue (EMB)
E.coli colonies with green metallic sheen
charcoal yeast extract buffered with cysteine and iron
Legionella ( primarily intracellular)
Sabouraud’s agar
Fungi
Giemsa stain
chlamydia borrelia rickettsiae trypanosomes plasmodium
PAS (periodic acid-Schiff)
stains glycogen - mucopolysaccharides
dx: Whipple’s disease = Tropheryma whipplei
india ink
cryptococcus neoformans
silver stain
fungi (Pneumocystis jireveci)
Legionella
H. pylori
congo red
amyloidosis
apple-green birefringence
Ziehl-Neelsen (carbol fuchsin)
acid fast stain - Nocardia, Mycobacterium
high lipid content in cell wall
poor gram stainers
treponema - too thin Ricketsia = intracellular Mycobarteria = acid fast Mycoplasma = no cell wall Legionella = primarily intracellular Chlamydia = intracellular
MacConkey’s agar components
bile salts & crystal violet = inhibit gram +
lactose
neutral red stain (stain + lactose fermenters PINK)
obligate intracellular organisms
rickettsia
chlamydia
- can’t make own ATP
facultative intracellular
salmonella neisseria brucella mycobacterium listeria fracisella legionella yersinia pestis
encapsulated bacteria
SHiNE SKiS step pneumo haemophilus influenza type B neisseria meningitidis E. coli salmonella Klebsiella group B Strep
Quellung reaction
encapsullated organisms = SWELLING
obligate aerobes
Nocardia
Pseudomonas aeruginosa
Mycobacterium tuberculosis
Bacillus
obligate anaerobes
Clostridium
Bacteroides
Actinomyces
catalase + organisms
Pseudomonas Listeria Aspergillus Candida E.coli Staph aureus Serratia
urease positive bacteria
cryptococcus H. pylori Proteus Ureaplasma Nocardia Klebsiella Staph epidermidis Staph saprophyticus CH- PUNKSS
bacteria that produce pigments
Actinomyces israelii - yellow “sulfur” granules
Staph aureus- yellow pigment “golden colonies”
Pseudomonas aeruginosa = blue-green pigment
Serratia marcescens = red pigment
bacteria with protein A
Staph aureus
binds Fc region Ig = prevents opsonization & phagocytosis
IgA protease virulence factor
cleaves IgA = colonize respiratory mucosa Strep pneumoniae H. influenza type B Neisseria (SHiN)
M protein virulence factor
group A steptococci
helps prevent phagocytosis
