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Microbiology Flashcards

1
Q

most common cause infxn caused by this org. a localized skin infxn and may resemble streptococcal erysipelas

A

Erysipeloid

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2
Q

What organism is described when mot are workers handling fish and animal products and the route of infxn through cuts, scratches and puncture wounds

A

Erysipelothrix rhusiopathiae

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3
Q

T/F. On gelatin stab culture in demonstrating E. rhusiopathiae, its char. test tube brush like that grows at 37 degree C

A

False. 22C

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4
Q

Which of the ff. are not associated with Arcanobacterium haemolyticum?

a. rudimentary branching
b. + CAMP reaction
c. primarily asso. with acute pharyngitis
d. phospholipase D

A

B. + in reverse CAMP rxn.

positive rxn is arrow head zone WITHOUT hemolysis.

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5
Q

T/F. Phopholipase D is the inhibitor of B-Lysin which is the responsible for beta-hemolysis in CAMP test

A

true

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6
Q
  1. It is the msot common vaginal infxn in women of child bearing age.
  2. What is the causative agen of this infxn?
  3. What test is described wherein it is a non-cultural test to detect the causative agent causing the infection stated in number 1?
A
  1. Bacterial vaginosis
  2. Gardnerella vaginosis
  3. KOH test or WHIFF Test
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7
Q
  1. What is the normal pH of vagina?
  2. Wht organism is responsible for the normal pH of vagina?
A
  1. <4.5
  2. Lactobacillus
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7
Q

Identify what organism is being described based on its biochemical test

catalase - negative
oxidase - negative
hippurate hydrolysis - postive

A

Gardnerella vaginalis

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7
Q

Positive for Whiff Test

A

Fishy-Amine Odor

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7
Q

A bacteria that has the same characteristic as fungi

A

Nocardia brasilienses

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7
Q

What is being observed microscopically that aids the diagnosis for BV

A

clue cells

> > large squamous EC with gram variable bacilli and coccobacilli clustered on the edges

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7
Q

All but one is true about the culture characteristics of G. vaginalis

a. spx is vaginal discharge
b. facultative aerobic at a temperature of 35-37C
c. MOC: Human Blood bilayer Tween agar
d. amphoterecin B is added to HBT

  1. What is the purpose of addition of amphotericin B?
A

B. capnophilic 5-7% of CO2

  1. to inhibit the growth of fungi and yeast
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7
Q
  1. What are the two nocardia organism that is commonly infect human?
  2. Wht is the infection of this organism that result to bronchopneumonia that is usually chronic
  3. Majority of this infection is caused by N.brasiliensis
  4. Term used to differentiate a bacteria that has the same char. as fungi. It is char. by swelling, drainig sinuses, pus containing Sulfur granules
A
  1. N. asteroides and N.brasiliensis
  2. Pulmonary Infxn
  3. Cutaneous infxn
  4. actinomycotic mycetomas
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7
Q

Odd one out
a. Actinomadura
b. Nocardiopsis
c. Nocardia
d. Dermatophilus

A

Partially acid-fast aerobic actinomyces:
GoRa NaTa!!
G = Gordonia, R = Rhodococcus, N= Nocardia, T= Tsukamurella

Non-Acid Fast aerobic actinomyces:
No SAD!!
N= Nocardiopsis, S=Streptomyces, A=Actinomadura, D=Dermatophilus

Ans. C

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7
Q

A phenomenon that helps in the recognition of Haemophilus spp that require V factor

A

Satellitism

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7
Q

what are the 3 haemophlius spp that is usually infect humans

A

H. influenzae
H. aegyptus
H. ducreyi

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8
Q
  1. What is the other name for H. influenzae?
  2. Serotype of organism stated above that causes most of the infection of humans.
  3. WHat haemophilus spp is described when MOT is respiratory secretions
  4. Other name for H. influenzae biotype aegyptus
A
  1. Pfeiffer’s bacillus
  2. H. influenzae Serotype B
  3. H. influenzae
  4. Koch-Weeks bacillus
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8
Q

What decolorizer is added to a bacteria that is normal inhabitants of soil and water and primarily responsibe of plant material?

A

0.5% to 1% SULFURIC ACID

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8
Q

Haemophilus spp that requires both X and V factor

A

H.influenzae
H.aegyptus
H.haemolyticus

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8
Q

Causative agent for soft chancre

A

Haemophilus ducreyi

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8
Q

causative agent of hard chancre

A

Treponema pallidum

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8
Q

causative agent for meningitis of children aged 3 months to 6 years old

A

H. influenzae

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8
Q

causative agent for meningitis in neonatal

A

S. agalactiae

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8
Q

causative agent for meningitis in children but seen mostly in elders

A

Listeria monocytogenes

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9
Q

All but one described culture media for Haemophilus spp.

a. CHOC agar with bacitracin (300 mg/dL)
b. Incubation is between 35-37C in aan atmosphere of 5% to 10% co2
c. 5% Horse or Rabbit Blood Agar
d. MacConkey agar

A

D. cannot grow on MacConkey kay it cannot produce V and X factor

9
Q

schools of fish

A

Haemophilus ducreyi

10
Q

HAemophilis spp that only requres V Factor

A

H.parainfluenzae
H.parahaemolyticus
H.paraphrohaemolyticus

11
Q

Haemophilus spp that only requires X factor

A

H. ducreyi

12
Q

test is based on the ability of the org to convert the substrate delta-aminolevulinic acid (ALA) into porphyrins or porphobilinogen

What are the Haemophilus spp that are porphyrins postive

What is the positive result for Porphobilinogen

A

Porphyrin Test

H. parainfluenzae
H.parahaemolytics
h.paraphrohaemolytics

red color upon addition of PDAB or KOVACS

13
Q

All but one refers to the group of HACEK bacteria

a. facultative anaerobic
b. dysgonic
c. attachment to heart valves
d. normal bacteria of the oral cavity
e. opportunist

A

a. capnophilic

14
Q

Identify bacteria in HACEK group based on biochemical test

catalase - neg
oxidase - v
glucose pos
maltose pos
sucrose pos
lactose pos

A

Aggregatibacter aphrophilus

the only lactose positive in hacek grp

15
Q

Identify the bactwria in HACEK group based on biochemical test

catalase postivie
oxidase v
glucose pos
maltose v
sucrose neg
lactose neg

A

Aggregatibacter actinomycetemcomitans

the only catalse positive in hacek grp

16
Q

Identify the bactwria in HACEK group based on biochemical test

catalase neg
oxidase pos
glucose neg
maltose neg
sucrose neg
lactose neg

A

eikenella corrodens

the only glucose negative in hacek group

17
Q

Identify the organism being described.

  1. human tissue infxn have been attributed to bites by cattle, horse, sheep or bigs
  2. Infects the aortic vavle more frequently than other HACEK group
A
  1. Aggregatibacter actinomycetemcomitans
  2. Cardiobacterium hominis
18
Q

identfiy organism being described

  1. It is imp pathogen in the pedia popu and have predilection for bones and joints
  2. Mixed infections that occur as a reault of trauma, esp. after human bites or fights
    3.most common cause of osteoarthritis infxn in children younger than 4 years of age
A
  1. Kingella
  2. Eikenella corrodens
  3. Kingella
18
Q

common physical findings in brucellosis

A

lymphadenopathy and splenomegaly

18
Q

bacteria that causes undulant fever

A

brucella

19
Q

brucellosis also known as

A

bang’s disease, mediterranean fever and malta fever

20
Q

what media is used in brucellosis that has a biphasic system containing broth and agar

A

Castaneda Bottles

21
Q
  1. a most common isolated brucella
  2. A brucella spp that is positive growth on media containing thionin and basic fuchsin
A
  1. Brucella abortus
  2. Brucella melitensis
22
Q

Brucella that is rapid urease produicer

A

B. suis and B. canis

23
Q

A bacteria that is most commonly infected to humans through unpasteurized milk product

A

Brucella

24
Q

BCYE with L-cysteine is best isolation for ?

A

Legionella

Buffered-Charcoal Yeast Extract

25
Q

Whooping cough

A

Bordetella

26
Q

mercury droplets colonies

A

BOrdetella

27
Q

safety pin appearance when stained

A

C. diptheriae

28
Q

Gram neg rods; described as “curved” or straight rods; has rapid darting or shooting star motility

A

Vibrio

29
Q

summer diarrhea

A

Vibrio parahaemolyticus

30
Q

T/F. All Vibrio are halophilic except V. mimicus and V. vulnificus

A

F. except V. mimicus and V. cholerae

31
Q

T/F. string test used to differentiate aeromonas and vibrio where aeromonas is positive for string test upon addition of sodium deoxycholate

A

False. Vibrio is positive to string test

32
Q

most common cause of bacterial gastroenteritis worldwide

A

Campylobacter jejuni subp jejuni

33
Q

sea-gull wing shape

A

Campylobacter

34
Q

Incubation for C jejuni subsp jejuni
Incubation for C fetus subsp fetus

A

42C
37C

35
Q

What test used to differentiate Helicobacter from Arcobacter and Campylobacter?

A

Urease positive Helicobacter

36
Q

corn tortilla like odor

A

Pseudomonas aeruginosa

37
Q

A positive and neg resulto of Cetrimide agar

this agar is used for what bacteria

A

positive - bluie green
neg - yellow green

Pseudomonas aeruginosa

38
Q

Identify wht categories of Diarrheagenic E. coli

  1. travelers and chilhood diarrhea
  2. infantile diarrhea
  3. dysentery
A
  1. ETEC
  2. EPEC
  3. EIEC
39
Q

other name for Klebsiella pneumoniae

A

Friedlander’s bacillus

40
Q

what case Bacillary Dysentery

A

Shigella dysenteriae

41
Q
A

board exam (9 decks)

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