microbiology 1 Flashcards
the study of unicellular organisms
microbiology
what organisms does microbiology consist of
bacteria, viruses, and fungi
how long is bacteria
0.5-5 microns
what stain is used in microbiology
gram +
what is bacteria classified by
genus and species
what is bacteria named for (2 major)
color and shape
how else can bacteria be named
referance to discovery location, discoverer, or infection caused
ridged outer layer of bacteria cell; gives shape and protection
cell wall
what organism lacks a cell wall
mycoplasma
selectivly semi-permiable bacteria structure
cell membrane
bacteria structure that contains life sustaining nutrients
cytoplasm
bacteria structure used for cell devision
mesosomes
gelatenous covering around the outside of the bacteria cell; causes it to be anti-phagocytic
capsules
whip-like bacteria structure used for locomotion
flagella
bacteria structure used to facilitate adhearance of bacteria to mucus membranes
pilli or fimbiria
bacterial stage to cause resistance
spores/ endospores
what bacterial structure stage can survive pasteurization
spores/ endospores
what occurs when bacteria are in an unfavorable location
spores/ endospores
bacteria source of energy
carbon and nitrogen
how does bacteria aquire nitrogen
protein matabolizm
what % water does bacteria require
7-10
what pH does bacteria require
7-7.5
what temp. does bacteria require
35-37 C
at what temp do nearly all pathogenic bacteria to animals grow
20-40 C
asexual reproduction
binary fission
able to grow well in the presence of oxygen; must have oxygen
aerobic
cant grow in the presence of oxygen; grow through fermentation
anaerobic
what oxygen tolerance has an odor
anaerobic
can grow with or without oxygen
facultative
grows best in small amounts of oxygen with increased amounts of carbon dioxide
microaerophilic
mesophilic bacteria
pathogenic 20-40 C
psychrophiles
cold loving 0-30 C
thermophiles
heat loving 40-80 C
define ubiquitous
everywhere
what can E. coli in the bladder cause
cystitis
where is normal flora found (4)
GI, trachea & bronchi, skin, urinary tract
what vitamin does normal flora produce
K
how does normal flora prevent disease
microbial antagonism
normally do not produce disease but can under certain circumstances if found in an unusual location
opportunistic pathogen
never normal flora; if present in/on an animal, will cause disease
obligate pathogen
bacteria invade the tissue, destroy cells, and cause disease
tissue invasion
what are the 2 types of toxin producers
exotoxins and endotoxins
produce toxin in the cell wall and secrete toxin into the host
exotoxins
what toxin producer causes the cell to die
exotoxins
produce toxin in the cell wall and causes the cell wall to rupture
endotoxins
botulism and tetnus are examples of _____
exotoxins
e. coli, salmonella, and bordatella are examples of _____
endotoxins
examples of indirect transmission
resp. route or ingestion
fomite route of transmission
inanimate object
parenteral route of transmission
transfusions
competes with pathogens for sites of attatchment
normal flora
barrier to repel invaders
intact skin
trap bacteria making them easier to be phagatosized
mucous membranes
B and T cells cause what
immune response
to obtain a sample from a patient that is representative of the disease process
specimen collection goal
sample collected ASAP after death and prior to administration of antibioics
optimal time
sample representitive of the whole
aliquots
how much tissue should be collected for a sample
more that 3 square cm
how much body fluid should be obtained for a sample
more that 1 mL
what is a sterile swab used to collect
urine cultures
swab transport system that has a medium at the bottom
culturette
culturette aka
copan
what are non sterile swabs used to collect
skin, mucus membranes, abssess
when is it best to collect blood samples
temp spike
difficult to culture due to low bacteria level
blood
how often should blood be drawn for collection
every 2 hours
usually preformed if bacteria and wbc are seen after spinal tap culture
CSF
determine bacterial arthritis
synovial fluid
obtained in necropsy or surgery and stored in 10% formalin
tissues
what are endotoxins usually a product of
gram - bacteria