Microbio lecture 2 Flashcards
Microbiology range
micrometers, nanometers, millimeters
Evidence for microbes existence
Fossil, some visible via microscopes
All microbes
- Enclosure/ “body”
- Genetic Material = DNA or virus have RNA or DNA
Types of Microbes
Eukaryotes, Viroids, Prokaryotes
Prokaryotes
Single cell organism that lacks a nucleus and lacks the majority of other membrane bound organelles (except ribosomes)
-Chromosomes
-Ribosomes
- some have flagellum
- cell membrane
- Most have a cell wall
- DNA
Eukaryotes
Largest of all types microbes, they contain membrane bound organelles
Ex:
Algae, protozoa, Fungi
Viruses
Not technically Alive, because they require a host to form a parasitic relationship with. They are also very simple and have a small structure, therefore they are more challenging to study.
Spontaneous Generation
The belief that non living matter can arise from living matter.
Anton van Leeuwenhoek
Develops first microscopes and discovers the microbial world
Importance of discoveries:
Novel Technology
Microscopic life exists
Scientific Method
The approach that anyone can use to explain a natural phenomenon using a lengthy process of experimentation, analysis, collecting data, and assessing whether the data supports or refutes the hypothesis.
Louis Pasteur
Realized that microbes are everywhere, father of germ theory, and he performed the infamous swan flask experiment
Swan flask experiment
He put nutrient broth in two flasks and kept one sealed and one unsealed. He then boiled both flasks and notice that the open flask had growth and the sealed one had no growth. He then broke open the sealed flask and noticed that it had growth. This overturned spontaneous generation
Key findings:
Pasteurization
Germ theory of disease
First vaccines developed
Pasteurization
The sterilization of not only milk/food, but also medical instruments
Robert Koch
Solidified Germ theory, because he noticed symptoms of infection in human, transferred the microbe, isolated it, transferred it to a healthy animal and then re isolated the microbe.
Keyfinding:
First conclusive evidence that bacteria are the causative agents of some diseases
Lead to development of Koch’s postulates (set of four principles)
Koch’s postulates
- Microorganism must be found inn abundance in all organisms suffering from the disease, but should not be found in healthy organisms
- Microorganism must be isolated from a diseased organism and grown in pure culture
- The cultured Microorganism should cause disease when introduced to a healthy organism
- The microorganism must be re isolated from the inoculated, disease experimental host and identified as being identical to the original specific causative agent