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ACTS (Reinforcement) > Microbio and Para Part 2 > Flashcards

Microbio and Para Part 2 Flashcards

1
Q

Which of the following specimen collection technique is stated correctly?

a. Respiratory specimens, such as sputum and Bronchoalveolar lavage are collected in clean, screw top container
b. Stool for routine culture is collected in sterile, leakproof container
c. CSF collected in sterile, screwcap tube should be submitted within 30 mins of collection
d. Most swab specimens can be stored at 24 hours at room temp

A

(D)

A and B baligtad container

C - less than 15 mins of collect

Outer ear swab should be stored at 4 C for 24 hours

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2
Q

The lowest concentration of antibiotic that inhibits growth of a test organism is the:

a. MIC
b. SIC
c. MBC
d. MIT

A

a

C- kills

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3
Q

Which of the following bacteria should be considered important pathogens when reading Gram-stainded smears of soft tissue Abscess?

a. S. pneumoniae
b. N. gonorrhoeae
c. Pseudomonas aeruginosa
d. S. auerus

A

D

P. aeruginosa is for immunocompromised opportunistic

MRSA = Vancomycin (Red man syndrome)

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4
Q

Ideal AST methods use ___ McFarland standard

a. 5
b. 0.5
c. 0.05
d. 50

A

b

0.5 McFarland = 1.5 x 10^8

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5
Q

In the kirby bauer disk diffusion susceptibility test, which variable is critical when testing Pseudomonas spp. for Antibiotic susceptibility to Aminoglycosides

a. Incubation Temp
b. Duration of incubation
c. Cation content of media
d. Depth of Agar

A

C

Imbalance of cations causes the negative charges of P. aeruginosa to be covered up making it False resistance

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6
Q

After satisfactory performance of Daily disk diffusion susceptibility QC is documented, the frequency of QC can be reduced to:

a. Twice a week
b. Every Week
c. every other week
d. Every month

A

B

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7
Q

According to the kirby bauer standard antimicrobial susceptibility testing method, what should be done when interpreting the zone size of a motule, swarming org. such as proteus spp.

a. The Swarming area should be ignored
b.
c.
d.

A

a

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8
Q

Which of the following if the most appropriate org. and media combination?

a. Legionella - Regan Lowe
b. Clostridium - PEA
c. Campylobacter - BYCE
d. Yersinia Enterocolitica - CIN

A

d

CIN is derived from MaConkey

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9
Q

Upon Review of sputum gram stain, the technician notes that the nuclei of all of the neutrophils present in the smear ae staining dark blue

a. The slide was inadequaltely decolorized with acetone / alcohol
b. The sputum smear was prepared too thin
c. The cellular components have stained as expected
d. The iodine was omitted from the staining procedure

A

a

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10
Q

CHOC agar base containing Vancomycin, colistin anisomycisn and trimethroprim lactate is also known as

a. EMB agar
b. MTM agar
c. Columbia CNA agar
d. MARTIn Lewis Agar

A

d

Colistin - inhibits all neg exceopt Neisseria and Proteus

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11
Q

Which one of the following organisms does not require susceptibility testing to the antimicrobial indicated when isolated from clinically significant source?

a. S. aureus - Clindamycin
b. P. mirabilis - Gentamicin
c. S. pyogenes - penicillin
d. E. coli - Levofloxacin

A

c

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12
Q

Diagnosis of typhoid fever can be confirmed best by culture of:

a. Stool
b. Urine
c. Blood
d. Bone marrow

A

C

1st week = Blood
2nd wee = Stool
3rd week = Urine
5th week = Serum

BSUS

Chronicity happens in Gallbladder

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13
Q

Name in order the four reagents used in Gram stain

a. CV, 95% Alcohol, iodine, Safranin
b. CV, 95% Alcohol, iodine, Safranin
c. CV, 95% Alcohol, iodine, Safranin
d. CV, iodine, 95% Alcohol, Safranin

A

d

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14
Q

Cycloserine-Cefoxitin Fructose agar is used for the recovery of?

a. Yersinia enterocolitica
b. Yersinia intermedia
c. C. perfringens
d. C. difficile

A

d

Ground glass appearance

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15
Q

A positive test using SCA would be indicated by the following color:

a. Orange
b. Yellow
c. Red
d. Blue

A

D

Simmon’s Citrate Agar

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16
Q

The fite faraco acid fast stain is different other acid fast stains because it uses:

a. Hematoxylin Rather than methylene Blue as counter stain
b. Carbol gential violet rather than safranin as a counter stain
c. Malachite green rather than hematoxylin as a counterstain
d. India ink with no counterstain

A

a

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17
Q

Medusa head colonies are ca characteristic of:

a. E. rhusiopathiae
b. B. anthracis
c. C. perfringens
d. S. moniliformis

A

b

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18
Q

What is the indicator used in urease test?

a. Phenol red
b. Neutral red
c. Bromothymol Blue
d. Bromcresol purple

A

A

MXCUT

Mannitol salt
XLD
CTA
Urease test
TSI

PPM - (2-4 hrs) rapid urease producer

B. SMAC
c. CHON

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19
Q

Which single test best seprates K. oxtoca from K. pneumoniae

a. Urease
b. Sucrose
c. Citrate
d. Indole

A

d

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20
Q

A positive OX K and Neg Ox-2 and OX 19 in the Weil-Felix test are associated with:

a. Rickettsial pox
b. Trench fever
c. Scrub typhus
d. Murine Typhus

A

c

Oriental tsutsugamushi

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21
Q

In the bacteriological analysis of water, ther confirmed test consist of:

a. Demonstration of gram neg, non-sporulating Rods from an agar slant
b. Gas in lactose tube
c. Typical coliform colonies on EMB agar Plates
d. Acid and Gas in lactose tubes

A

C

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22
Q

A Laboratorian properly dressed in white pants, lab coat, and shoes, prepares to leave the the lab for lunch, in addition to wash his hands, what did he forget?

a. Put on safety goggles
b. Removes his lab coat
c. Wipe the bench with water
d. Remove polyvinyl Gloves and place t hen into lab coat for future use

A

B

23
Q

The optochin disk is used for the identification of?

a. Haemophilus influenzae
b. Group A beta-hemolytic Streptococci
c. S. pneumoniae
d. Alpha-hemolytic streptococci

A

c

Also known as Taxo P

24
Q

TSI: K/A No H2S
Indole (-)
VP (+)
Citrate (+)
DNASE (+)
UREASE (-)
Gelatin Hydrolysis (+)
PAD (-)

a. Proteus vulgaris
b. Serratia marcescens
c. Proteus mirabilis
d. E. cloacae

A

B

SMS = DNASE (+)

25
Q

What reagent is used for PAD?

a. 10% ferric chloride
b. Kovac’s reagent
c. 10% KOH
d. 10% Sodium Deoxycholate

A

A

Phenylalanine deaminase

26
Q

All of the following Mycobacterium spp. produce the enzyme required to convert niacin to niacin ribonucleotide except:

a. M. kansasii
b. M. tuberculosis
c. M. avium
d. all of these

A

B

unique for M. tuberculosis

Canary YELLOW (+) indicator

27
Q

Bacillus anthracis and B. cereus can best be differentiated by which tests?

a. Motility and Beta hemolysis on blood agar plate
b. Oxidase and A -hemolysis on blood agar plate
c. Lecithinase and glucose
d. Lecithinase and catalase

A

a

28
Q

Which of the following specimens would be best for identifyin bacillus cereus as the cause of an outbreak pf food posioning?

a. Blood
b. Rectal Swabs
c. Stool Samples
d. Food

A

D

29
Q

All species of the Genus Neisseria have the enzyme to oxidize:

a. Napthylamine
b. Dimethylaminobenzaldehyde
c. Glucopyranoside
d. tetramethyl-phenylenediamine

A

d

30
Q

Which of the following are a common element between using the E test and Agar disk diffusion for antimicrobial susceptibility testing?

a. Both establish an Antibiotic gradient in agar
b. Both create a circular zone of bacterial infection
c. Expression of results is the MIC for broth
d. The cost of the test is similar per drug

A

a

31
Q

Tracking the rate of skin organism contamination among a laboratory’s blood culture results on a monthly basis and introducing specific training to phlebotomists when rates exceed the norm would be an example of which of the following?

a. Good Lab practices
b. Quality control
c. Universal standards
d. Quality assurance

A

D

A - GLP means

32
Q

GS and reading a glass slide with a mixed smear of Staphy and E. coli along with each GS run of specimens examined within the microbiology lab that day is an example of?

a. QA activity
b. QC activity
c. National regulatory activity
d. RITM NEQAS activity

A

b

33
Q

A helminth egg with 2 polar plug (Barallel shape / Japanese lantern)

a. C. philippinensis
b. T. trichuria
c. A. lumbricoides
d. E. granulosus

A

b

34
Q

Artifacts in a stool specimen that can be confused with ova or cyst are:

a. Partially digested Meat fibers
b. Degenrated cells from the gastrointestinal mucosa
c. Dried chemical crystals
d. Pollen grains

A

d

35
Q

Polyvinyl alcohol used in the preparation of permanently stained smears of fecal material:

a. Concentrates eggs
b. Dissolves artifacts
c. Serves as an adhesive
d. Enhances stain penetration

A

c

36
Q

All of the followin are spiral shaped except:

a. Clostridium botulinum
b. H. pylori
c. T. pallidum
d. V. cholerae

A

a

37
Q

The presence of residual chlorine in drinking water means:

a. Sufficient amount of chlorine was added initially to the water
b. Water is unsafe for drinking
c. Additional treatment is required to remove chlorine
d. Water is contaminated with coliform

A

a

38
Q

Acquired through ingestion/inhalation

a. A. lumbricoides
b. whipworm
c. E. vermicularis
d. S. stercoralis

A

c

2 CHESI - Autoinfection

39
Q

How to validate the result of AST?

a. parallel testing with a new method
b. Parallel testing with another personnel
c. Testing with a reference reagent
d. Testing with a reference organism

A

B

40
Q

what test is employed in the differentiation of the Enterobacteriaceae family?

a. IMViC
b. Indole
c Glucose fermentation
d.. Capsule

A

a

41
Q

Best time to collect E. vermicularis ova

a. Morning
b. Afternoon
c. Night
d. Midday

A

a

42
Q

How parasitisim is expressed when malaria parasite is quantitated against RBC?

a. %Parasitemia
b. Parasites/microliter of blood
c. Ratio
d. Mean

A

a

IF RBC = A
IF WBC = B

43
Q

Which type of organism isolated if growth in MAC is pink

a. Non lactose fermenter
b.
c. Lactose fermenter
d.

A

c

44
Q

Method that uses heat to facilitate better staining of bactrial cell walls

a. Gram stain
b. Kinyoun
c. Ziehl-nielsen
d. Acid fast stain

A

c

45
Q

Specimen is obtained from an intravsucular catheter. GS showed Gram positive bacteria

a. S. auerus
b. E. coli
c. P. aeruginosa
d.

A

a

46
Q

In Mac agar, what is the color of the lactose

a. Black
b. White
c. Red
d. Pink

A

d

47
Q

Babes-ernst granules:

a. M. tuberculosis
b. Y. pestis
c. C. diptheriae
e. E. aerogenes

A

c

48
Q

What is the pre analytical part of microbiology test>

a. Standardization of parasite namine
b. Counterchecking slides
c. Proper storage of reagents and stains
d. Identify patient

A

d

49
Q

What is the characteristic odor of proteus

a. grabe like / Corn tortilla odor
b. Bleach like odor
c. Fruity odor
d. Burnt chocolate odoer

A

d

A = P. aeruginosa

50
Q

What is the most sensitive test for chlamydia

a. NAAT
b. Cell culture
c. EIA
d. Microscopy

A

a

Specimen: Urine

51
Q

Happens during microorganisms resistance except:

a. Ag-Ab complex
b. Penetration of membrane protein
c. Reduced antimicrobial therapy
d. By Passing the membrane protein

A

a

52
Q

Positive result of indole

a. Red Ring
b. Black Ring
c. Red clumps
d. Black clumps

A

a

53
Q

Nitrogen source

a. Yeast Extract
b. Peptone
c. Beef extract
d. Meat extract

A

b

54
Q
A

ACTS (Reinforcement) (13 decks)

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