Microbial Nutritional Requirements and Transport Flashcards

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1
Q

Give 6 required non-metal and 4 required metal macronutrients

A

Oxygen, Carbon, Nitrogen, Phosphorus, Sulphur and Hydrogen
K, Mg, Ca, Fe

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2
Q

Give 2 examples of required micronutrients - how do these differ from macronutrients

A

Co + Cu
Absence will not cause growth to stop

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3
Q

Define and give 1 disadvantage for both prototrophy and auxotrophy

A

Prototrophy - Able to synthesise all of its cellular constituents from basic nutrients
Disadvantage - must maintain and multiply the genes for all of these processes
Auxotrophy - Lost ability to make certain compounds required for growth and metabolism
Disadvantage - Running out of nutrients causes growth to stop

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4
Q

What are the 3 things core to metabolism

A

Energy, Electrons and Carbon

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5
Q

2 ways in which microbes acquire energy and the given name

A

ATP from photosynthesis - Phototrophs
Organic/Inorganic compounds - Chemotrophs

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6
Q

3 ways in which energy is used

A

Chemical work
Transport work
Mechanical work

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7
Q

2 ways in which microbes acquire electrons and the given name

A

Inorganic compounds - Lithotrophs
Organic molecules - Organotrophs

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8
Q

2 ways in which electrons are used

A

Redox reactions - alter oxidation state
Energy generation by ETC

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9
Q

2 ways in which microbes acquire carbon and the given name

A

Co2 - Autotrophs
Organic carbon molecules - Heterotrophs

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10
Q

2 ways in which carbon is used

A

Building of structures
Act as a backbone

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11
Q

Order in which a metabolic microbe is named

A

Energy - Electron - Carbon

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12
Q

Define Passive diffusion

A

Movement of molecules from a high concentration gradient to a low concentration gradient

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13
Q

What is used in facilitated diffusion - 1 advantage to passive diffusion

A

Carrier molecule - permease
Allows transport of sugars and amino acids

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14
Q

Why is a plateau reached in facilitated diffusion

A

Carrier solution effect - permease becomes saturated

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15
Q

Does facilitated or passive diffusion have a faster initial rate

A

Facilitated

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16
Q

What form does the energy take in primary and secondary active transport

A

Primary - ATP
Secondary - Proton motive force

17
Q

What active transporter is used in primary active transport - What kind of transport protein is it

A

ABC transporter
Uniporter

18
Q

What are the outcomes of a build up of protons on one side of a membrane

A

pH difference
Electrical difference

19
Q

How does PMF support movement of molecules in symport

A

The proton can move across a membrane into a -vely charged space with a neutral or -vely charged molecule

20
Q

How does PMF support movement of molecules in antiport

A

The proton can ‘switch places’ with a anion or neutral molecule in a -vely charged space

21
Q

How does PMF support movement of molecules in uniport

A

Cation or anion is repelled by surrounding charges and enters or exits the cytoplasm

22
Q

What is electrogenic and electroneutral transport

A

Genic - transport that leads to a change in charge across the membrane
Neutral - Transport that does not lead to a change in charge across the membrane

23
Q

3 general steps in the carbon cycle

A
  1. Autotrophs use CO2 in the atmosphere to build cells
  2. Autotrophs die and the carbon is trapped in the ground which will be released by decomposers back into the atmosphere
  3. Autotrophs are eaten and CO2 is respired by heterotrophs
24
Q

Outline the 4 steps in the nitrogen cycle

A
  1. Nitrogen fixation - Nitrogen gas is reduced to ammonia then to organic nitrogenous molecules
  2. Ammonification - Organic nitrogenous molecules are converted back into ammonia
  3. Nitrification - Ammonia is oxidised to form nitrite which is further oxidised to form nitrate
  4. Denitrification - Nitrate is reduced back into nitrogen gas and released
25
Q

Outline the 4 steps in the sulphur cycle

A
  1. Sulphur is washed to oceans by rain
  2. Algae consumes sulphur to form DMSP
  3. DMSP is broken down to produce DMS gas
  4. DMS dissolves in water in the atmosphere and is drawn back into the soil when it rains
26
Q

What is a defined medium

A

Medium when all components and their concentration are known

27
Q

What is a complex media and when is it used

A

Medium containing some ingredients of unknown composition and concentration
Nutritional requirements of a microbe are unknown

28
Q

What is a selective media - give 1 example

A

Media tailored to favour the growth of some microbes and inhibit the growth of others
MacConkey agar - favours growth of gram -ve bacteria

29
Q

What is the purpose of differential media

A

Allows you to distinguish between difference microbes based on their biological characteristics

30
Q

Give 3 examples of how microbial growth is applied industrially

A

Primary and secondary biofuels
Waste water treatment
Bioremediation and bioaugmentation