Bacterial DNA Replication Flashcards
What did Griffith’s experiment on transformation prove
Bacteria can transfer genetic information through a process called transformation
What was the conclusion of the Avery, Macleod and McCarty experiment and what did this prove
Transformation of the non-virulent (r strain) is only blocked when DNA is destroyed
This proves that the transforming substance in an organism is DNA
What did Hershey and Chase prove
DNA and not proteins carry the genetic information
Which enzyme separates and unwinds DNA at the OoR
DNA helicase
Which protein keeps the two strands separate
Single stranded DNA binding proteins
What does DNA polymerase first need before synthesising a new strand - what end is it on - what does this
A primer - 3’ - primase
What is the role of DNA polymerase I
Removes primers using exonuclease activity and replaces with nucleotides
Which enzyme joins the Okazaki fragments together
DNA ligase
Define a gene
A sequence of polynucleotides that code for one or more functional products
What is the difference between the sense and antisense strand
Sense - when read in the right direction it will code for a protein
Antisense - non-coding strand
During transcription how will the resulting mRNA strand differ from the sense strand
T will be replaced with U
Which sequence is the first to be transcribed into mRNA but will not be translated into an amino acid
Shine-Dalgarno sequence
What does the coding region begin with in the template strand
TAC
What is the name of the region that RNA polymerase binds to in transcription
Promoter region
What is the name of the sequence following the stop codon that is transcribed but not translated - what follows this?
Trailer
Terminator
