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Microbial Culture Technology Flashcards

1
Q

-cells are grown under controlled conditions, generally outside their natural environment.

A

CELL CULTURE

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2
Q

contains an appropriate vessel with a substrate or medium that supplies the essential nutrients.

A

CELL CULTURE

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3
Q

The original media used by __ – urine or meat broth

A

Louis Pasteur

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4
Q

macroscopically visible collection of millions of bacteria originating from a single bacterial cell

A

Colony

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5
Q

Cooked cut potato by __- the earliest solid medium

A

Robert Koch

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6
Q

not satisfactory; liquefy at 24oC

A

Gelatin

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7
Q

who introduced the agar medium

A

Franny Hesse

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8
Q

Used for preparing solid medium obtained from seaweeds

A

Agar

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9
Q

No nutritive value; Not affected by the growth of the bacteria.

A

agar

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10
Q

agar melts at __ and sets at __

A

Melts at 98oC;
sets at 42oC

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11
Q

Types of culture media (3)

A

Based on their consistency
Based on the constituents/ ingredients
Based on Oxygen requirement

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12
Q

Types of culture media Based on their consistency

A

a) solid medium
b) liquid medium
c) semi solid medium

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13
Q

Types of culture media Based on the constituents/ ingredients (4)

A

a) simple medium
b) complex medium
c) synthetic or defined medium
d) Special media

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14
Q

Special media (8)

A

◦ Enriched media
◦ Enrichment media
◦ Selective media
◦ Indicator media
◦ Differential media
◦ Sugar media
◦ Transport media
◦ Media for biochemical reactions

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15
Q

Types of culture media Based on Oxygen requirement

A
  • Aerobic media
  • Anaerobic media
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16
Q

contains 2% agar;
Colony morphology, pigmentation, hemolysis can be appreciated.

A

Solid media

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17
Q

example of solid media ((2)

A

nutrient agar, blood agar

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18
Q

no agar.; For inoculum preparation, Blood culture, for the isolation of pathogens from a mixture.

A

Liquid media

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19
Q

example of liquid media

A

Nutrient broth

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20
Q

0.5% agar.

A

Semi solid medium

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21
Q

example of Semi solid medium

A

Motility medium

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22
Q

example of simple/basal media (2)

A

NB, NA

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23
Q

NB consists of __, __ , __

A

peptone, meat extract, NaCl

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24
Q

NB + 2% agar =

A

nutrient agar

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25
They have added ingredients. Provide special nutrients
Complex media
26
Media prepared from pure chemical substances and its exact composition is known
Synthetic or defined media
27
example of Synthetic or defined media
peptone water – 1% peptone + 0.5% NaCl in water
28
Substances like blood, serum, egg are added to the basal medium.
Enriched media
29
Used to grow bacteria that are exacting in their nutritional needs.
Enriched media
30
examples of Enriched media (2)
Blood agar, Chocolate agar
31
Liquid media used to isolate pathogens from a mixed culture.
Enrichment media
32
Media is incorporated with inhibitory substances to suppress the unwanted organism.
Enrichment media
33
examples of enrichment media (2)
◦Selenite F Broth; Alkaline Peptone Water
34
◦Selenite F Broth – for the isolation of __, __
Salmonella, Shigella
35
The inhibitory substance is added to a solid media.
Selective media
36
examples of selective media (5)
-Mac Conkey’s medium -Thiosulfate–citrate–bile salts–sucrose agar (TCBS) -Löwenstein-Jensen (LJ) medium -Wilson and Blair medium -Potassium tellurite medium -
37
selective media for V. cholerae
Thiosulfate–citrate–bile salts–sucrose agar (TCBS)
38
egg-based medium; M. tuberculosis
Löwenstein-Jensen (LJ) medium
39
combination of bismuth and sodium sulphite ; S.typhi
Wilson and Blair medium
40
–inhibits the growth of most bacteria associated with Enterococcus species. Diphtheria bacilli
Potassium tellurite medium
41
These media contain an indicator which changes its colour when a bacterium grows in them
Indicator media
42
examples of Indicator media (3)
◦Blood agar ◦Mac Conkey’s medium ◦Christensen’s urease medium
43
____- pink colonies ____- colorless colonies
Lactose fermenters- pink Non lactose fermenters
44
Media used for transporting the samples.
Transport media
45
Delicate organisms may not survive the time taken for transporting the specimen without a _
transport media
46
examples of transport media (2)
Stuart’s medium Buffered glycerol saline
47
non nutrient soft agar gel containing a reducing agent
Stuart’s medium
48
enteric bacilli
Buffered glycerol saline
49
examples of Anaerobic media (2)
-Robertson’s cooked meat medium -Thioglycolate medium.
50
Culture methods include: (7)
Streak culture Lawn culture Stroke culture Stab culture Pour plate method Liquid culture Anaerobic culture methods
51
Used for the isolation of bacteria in pure culture from clinical specimens.
STREAK CULTURE
52
what wires are used in streak culture?
Platinum wire or Nichrome wire
53
Provides a uniform surface growth of the bacterium.
LAWN CULTURE
54
Uses of lawn culture (3)
◦bacteriophage typing. ◦ Antibiotic sensitivity testing. ◦preparation of bacterial antigens and vaccines.
55
are prepared by flooding the surface of the plate with a liquid suspension of the bacterium.
lawn cultures
56
___ is made in tubes containing agar slope / slant
Stroke culture
57
◦Provide a pure growth of bacterium for slide agglutination
stroke culture
58
Prepared by puncturing a suitable medium – gelatin or glucose agar with a long, straight, charged wire.
stab culture
59
uses of stab culture (3)
◦Demonstration of gelatin liquefaction ◦Oxygen requirements of the bacterium under study ◦Maintenance of stoke cultures.
60
Agar medium is melted (15 ml) and cooled to 45oC.; 1 ml of the inoculum is added to the molten agar.; poured to a sterile petri dish; Incubated at 37oC
POUR PLATE CULTURE
61
uses of pour plate culture (2)
◦ Gives an estimate of the viable bacterial count in a suspension. ◦ For the quantitative urine cultures.
62
are inoculated by touching with a charged loop or by adding the inoculum with pipettes or syringes.
Liquid cultures
63
uses of liquid culture (3)
◦ Blood culture ◦ Sterility tests ◦ Continuous culture methods
64
does not provide a pure culture from mixed inocula.
liquid culture

Cell & Mol Bio (7 decks)

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