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Cell & Mol Bio > Microbial Culture Technology > Flashcards

Microbial Culture Technology Flashcards

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Biology 101 flashcards
1
Q

-cells are grown under controlled conditions, generally outside their natural environment.

A

CELL CULTURE

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2
Q

contains an appropriate vessel with a substrate or medium that supplies the essential nutrients.

A

CELL CULTURE

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3
Q

The original media used by __ – urine or meat broth

A

Louis Pasteur

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4
Q

macroscopically visible collection of millions of bacteria originating from a single bacterial cell

A

Colony

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5
Q

Cooked cut potato by __- the earliest solid medium

A

Robert Koch

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6
Q

not satisfactory; liquefy at 24oC

A

Gelatin

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7
Q

who introduced the agar medium

A

Franny Hesse

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8
Q

Used for preparing solid medium obtained from seaweeds

A

Agar

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9
Q

No nutritive value; Not affected by the growth of the bacteria.

A

agar

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10
Q

agar melts at __ and sets at __

A

Melts at 98oC;
sets at 42oC

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11
Q

Types of culture media (3)

A

Based on their consistency
Based on the constituents/ ingredients
Based on Oxygen requirement

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12
Q

Types of culture media Based on their consistency

A

a) solid medium
b) liquid medium
c) semi solid medium

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13
Q

Types of culture media Based on the constituents/ ingredients (4)

A

a) simple medium
b) complex medium
c) synthetic or defined medium
d) Special media

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14
Q

Special media (8)

A

◦ Enriched media
◦ Enrichment media
◦ Selective media
◦ Indicator media
◦ Differential media
◦ Sugar media
◦ Transport media
◦ Media for biochemical reactions

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15
Q

Types of culture media Based on Oxygen requirement

A
  • Aerobic media
  • Anaerobic media
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16
Q

contains 2% agar;
Colony morphology, pigmentation, hemolysis can be appreciated.

A

Solid media

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17
Q

example of solid media ((2)

A

nutrient agar, blood agar

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18
Q

no agar.; For inoculum preparation, Blood culture, for the isolation of pathogens from a mixture.

A

Liquid media

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19
Q

example of liquid media

A

Nutrient broth

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20
Q

0.5% agar.

A

Semi solid medium

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21
Q

example of Semi solid medium

A

Motility medium

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22
Q

example of simple/basal media (2)

A

NB, NA

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23
Q

NB consists of __, __ , __

A

peptone, meat extract, NaCl

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24
Q

NB + 2% agar =

A

nutrient agar

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25
Q

They have added ingredients. Provide special nutrients

A

Complex media

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26
Q

Media prepared from pure chemical substances and its exact composition is known

A

Synthetic or defined media

27
Q

example of Synthetic or defined media

A

peptone water – 1% peptone + 0.5% NaCl in water

28
Q

Substances like blood, serum, egg are added to the basal medium.

A

Enriched media

29
Q

Used to grow bacteria that are exacting in their nutritional needs.

A

Enriched media

30
Q

examples of Enriched media (2)

A

Blood agar, Chocolate agar

31
Q

Liquid media used to isolate pathogens from a mixed culture.

A

Enrichment media

32
Q

Media is incorporated with inhibitory substances to suppress the unwanted organism.

A

Enrichment media

33
Q

examples of enrichment media (2)

A

◦Selenite F Broth;
Alkaline Peptone Water

34
Q

◦Selenite F Broth – for the isolation of __, __

A

Salmonella, Shigella

35
Q

The inhibitory substance is added to a solid media.

A

Selective media

36
Q

examples of selective media (5)

A

-Mac Conkey’s medium

-Thiosulfate–citrate–bile salts–sucrose agar (TCBS)

-Löwenstein-Jensen (LJ) medium

-Wilson and Blair medium

37
Q

selective media for V. cholerae

A

Thiosulfate–citrate–bile salts–sucrose agar (TCBS)

38
Q

egg-based medium; M. tuberculosis

A

Löwenstein-Jensen (LJ) medium

39
Q

combination of bismuth and sodium sulphite ; S.typhi

A

Wilson and Blair medium

40
Q

–inhibits the growth of most bacteria associated with Enterococcus species. Diphtheria bacilli

A

Potassium tellurite medium

41
Q

These media contain an indicator which changes its colour when a bacterium grows in them

A

Indicator media

42
Q

examples of Indicator media (3)

A

◦Blood agar
◦Mac Conkey’s medium
◦Christensen’s urease medium

43
Q

____- pink colonies
____- colorless colonies

A

Lactose fermenters- pink
Non lactose fermenters

44
Q

Media used for transporting the samples.

A

Transport media

45
Q

Delicate organisms may not survive the time taken for transporting the specimen without a _

A

transport media

46
Q

examples of transport media (2)

A

Stuart’s medium
Buffered glycerol saline

47
Q

non nutrient soft agar gel containing a reducing agent

A

Stuart’s medium

48
Q

enteric bacilli

A

Buffered glycerol saline

49
Q

examples of Anaerobic media (2)

A

-Robertson’s cooked meat medium
-Thioglycolate medium.

50
Q

Culture methods include: (7)

A

Streak culture
Lawn culture
Stroke culture
Stab culture
Pour plate method
Liquid culture
Anaerobic culture methods

51
Q

Used for the isolation of bacteria in pure culture from clinical specimens.

A

STREAK CULTURE

52
Q

what wires are used in streak culture?

A

Platinum wire or Nichrome wire

53
Q

Provides a uniform surface growth of the bacterium.

A

LAWN CULTURE

54
Q

Uses of lawn culture (3)

A

◦bacteriophage typing.
◦ Antibiotic sensitivity testing.
◦preparation of bacterial antigens and vaccines.

55
Q

are prepared by flooding the surface of the plate with a liquid suspension of the bacterium.

A

lawn cultures

56
Q

___ is made in tubes containing agar slope / slant

A

Stroke culture

57
Q

◦Provide a pure growth of bacterium for
slide
agglutination

A

stroke culture

58
Q

Prepared by puncturing a suitable medium – gelatin or glucose agar with a long, straight, charged wire.

A

stab culture

59
Q

uses of stab culture (3)

A

◦Demonstration of gelatin liquefaction
◦Oxygen requirements of the bacterium under study
◦Maintenance of stoke cultures.

60
Q

Agar medium is melted (15 ml) and cooled to 45oC.; 1 ml of the inoculum is added to the molten agar.; poured to a sterile petri dish; Incubated at 37oC

A

POUR PLATE CULTURE

61
Q

uses of pour plate culture (2)

A

◦ Gives an estimate of the viable bacterial count in a suspension.
◦ For the quantitative urine cultures.

62
Q

are inoculated by touching with a charged loop or by adding the inoculum with pipettes or syringes.

A

Liquid cultures

63
Q

uses of liquid culture (3)

A

◦ Blood culture
◦ Sterility tests
◦ Continuous culture methods

64
Q

does not provide a pure culture from mixed inocula.

A

liquid culture

Cell & Mol Bio (7 decks)

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