Micro - Lab interpretation Flashcards
What ae the causative agents involved in invasive Diarrhoea?
Salmonella spp.
Campylobacter spp.
Shigella spp.
E. coli
E. histolytica
What are the causative agents involved in Enterotoxin-Mediated Diarrhea?
- ETEC
- V. cholerae
- S. aureus
- C. perfringens
- B. cereus
What preservative should be used if there is a delay in submitting specimen to the laboratory?
Buffered glycerol saline
What test is used to differentiate invasive disease from toxin-mediated illnesses, viral illness and parasitic infections?
Fecal Leukocytes
If there is a positive Fecal Leukocyte what does it indicate?
The presence of WBCs and RBCs in faeces is due to intestinal wall bleeding
What is the culture media used for the isolation of Salmonella and Shigella?
- MacConkey’s Agar
- Xylose Lysine
Deoxycholate Agar
(XLD) - GN Broth
Fill in the blanks. “ In the culture of Shigella and Salmonella, Non- lactose fermenting colonies are removed and inoculated to __________.”
Kligler’s agar or Urease agar
What is the Kligler and Urea result for Salmonella spp.?
Kligler Alkaline Slant
H2S obscuring
Acid butt
Gas
Urea Negative
What is the Kligler and Urea result for Salmonella typhi?
Kligler Alkaline Slant
Acid butt
little H2S
No gas
Urea Negative
What is the Kligler and Urea result for Shigella app?
Kligler Alkaline Slant
Acid butt
Urea Negative
What is the agar used to isolate Campylobacter Jejuni and what is the duration for the culture?
Campylobacter blood agar (CBA) - Incubated for 48 hours at 42 degrees Celsius
What is the gram reaction for Bacillus cereus?
Gram positive sporulating bacilli
Which agar is used to identify Clostridium difficile?
Cefotoxin cycloserine fructose agar (CCFAgar)
Blood agar
What samples should be taken when investigating Enteric fever?
Blood cultures
Urine samples
Stool samples
Bone marrow culture
Clotted blood samples for serology
What organism is being investigated using the Widal test?
Salmonella typhi
