Micoscropy

Question 1

Define resolution

Answer 1

Ability to see two objects that are close together as separate objects, therefore ability to see detail

Question 2

Define magnification

Answer 2

The number of times larger an image is compared to the actual object

Question 3

What is resolution limited by?

Answer 3

The medium used in microscopes (wavelength of light)

Question 4

What is the formula for magnification?

Answer 4

Magnification = Image Size / Actual Size

Question 5

What is a stage micrometer?

Answer 5

A microscope slide that is engraved with an accurate scale to give reference to dimensions (DOES CHANGE). It is 100 nanometers long

Question 6

What is an eyepiece graticule?

Answer 6

A glass disc fitted into the eyepiece of the microscope which is marked with a fine scale of 0-100 (DOESN’T CHANGE)

Question 7

What is calibration?

Answer 7

The comparison of the stage micrometer to the eyepiece graticule. This is done by counting how many unmarked units of the eyepiece graticule fit into the stage micrometer then divide to get how many nanometers each line of the eyepiece graticule is worth