Mice Intro Flashcards

1
Q

Are rats of the genus Rattus and mice of the genus Mus bred for use in research covered by USDA regulation

A

No

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Are rats of the genus Rattus and mice of the genus Mus bred for use in research covered by PHS policy

A

Yes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Do mice have a gallbaldder

A

Yes (unlike rats)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

The red staining around a mouse’s mouth and nose is due to the accumulation of _____ produced by the _______ gland, a lacrimal gland

A

accumulation of porphyrins produced by the Harderian gland

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

A mouse in a recent shipment of animals appears dehydrated and you want to give SQ fluids. What is the max volume you can give to an average size mouse?

A

2-3 ml, up to 0.5 ml/site

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Continent of origin for the lab mouse?

A

Asia (Old World)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

The laboratory mouse genome is a mixture of what 2 species?

A

M. musculus and M. domesticus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the difference between axenic, gnotobiotic, SPF, and conventional mice?

A

Axenic- germ free
Gnotobiotic- only defined GI flora, no other bacteria
SPF- specific pathogen free, but normal commensal flora
Conventional- microbes not defined, can have pathogenic organisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the origin of the C3H strain?

A

From a cross of a Bagg albino female and a DBA male by Strong in 1920.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the color of C3H mice?

A

Agouti

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Typical adult mouse weight

A

20-40 grams

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Daily water consumption (ml/d) for a mouse?

A

6-7 ml/day

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Daily food consumption (g/d) for a mouse?

A

4-5 g/day

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Unique traits of murine urine include?

A

Urine is highly concentrated with large amounts of protein (0.5-1.0 ml in 24 hours)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Diploid chromosome number of the lab mouse? (2n= ?)

A

40

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Age of puberty for mice?

A

Puberty ~ 35 days (range 28-49 days)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Mice are polyestrous and cycle every ___ days

A

4-5

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is the significance of a vaginal plug in mice?

A

Denatured seminal fluid and sperm that signifies copulation has occurred.
80-90% of mice with vaginal plugs become pregnant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

When does the first post partum estrus occur?

A

12-24 hours after parturition

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

When does the post-lactation estrus occur?

A

2-4 days post weaning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Mouse gestation period

A

17-21 days

22
Q

Describe the number and location of mammary glands in the mouse.

A

5 pair of mammary fat pads just below the skin. 3 thoracic, 2 inguinal
Each has exterior nipple

23
Q

Cohoused male mice may fight. Typical fight injuries occur on which parts of the body?

A

Scrotal area, base of tail

24
Q

C57BL/6 mice are predisposed to what dermatological condition?

A

Idiopathic (ulcerative) dermatitis

Also barbering

25
Q

What the genus and species anme of the two major fur mites seen in lab mice?

A

Myobia musculi and Myocoptes musculinus

26
Q

Name the genuses of two common types of pinworms seen in mice.

A

Syphacia, Aspicularis

27
Q

Ringtail is associated with what environmental condition?

A

Low relative humidity

28
Q

Dental formula for mice

A

I 1/1 C 0/0 P 0/0 M 3/3

29
Q

Describe the approach for an IP injection in mice.

A

Lower right abdomen, between midline and hind leg

30
Q

Why are IM injections not recommended in mice?

A

Small muscle volume, injection can cause muscular atrophy, nerve damage

31
Q

Name six sites for blood collection in mice.

A
Medial saphenous vein
Lateral saphenous vein
Lateral tail vein
Submandibular
Retroorbital
Cardiac exsanguination
32
Q

Name 4 sites that are acceptable for blood collection awake in mice

A

Medial saphenous vein
Lateral saphenous vein
Lateral tail vein
Submandibular

33
Q

Ear punches or ear tags used for ID in mice should be placed where on the ear?

A

should be placed approximately 3 mm from the edge of the ear pinna.

34
Q

What 2 anatomical sites are commonly tattooed for individual mouse identification?

A

Tails and footpads

35
Q

When is toe clipping an acceptable means of identification in mice?

A

Only used when no other individual identification is feasible. 2011 Guide

36
Q

At what age should tail ampuation for mouse genotyping occur?

A

2-3 weeks of age

37
Q

What is the maximum amount of tail that should be amputated for genotyping?

A

2 mm

38
Q

How allometric scaling of drug doses performed?

A

Based on estimation of basal metabolic rate (BMR)
BMR = k(Wkg0.75)
Wkg= body weight in kilograms
k= “species factor”

39
Q

Which “old school” anesthetic is associated with peritonitis and a dynamic ileus?

A

Choral hydrate

40
Q

Which “old school” anesthetic is carcinogenic and, thus, unsuitable for survival surgeries?

A

Urethane

41
Q

This “old school” anesthetic was sold under the proprietary name Avertin and decomposes into irritating and toxic products if stored at room temperature and exposed to light?

A

Tribromoethanol

42
Q

Major disadvantages of pentobarbital for anesthesia?

A

Controlled (CII), poor analgesia, risk of perivascular necrosis

43
Q

According to ______’s Law a small change in airway diameter leads to big changes in airway resistence.

A

Poisseille’s Law

44
Q

Reducing the airway diameter of a rodent by 1/2 by ET tube placement, increases the resistance by how much?

A

16x

45
Q

What important consideration is essential for intubated small mammals?

A

Must be on ventilation

46
Q

Define major surgery

A

Major –“penetrates and exposes a body cavity, produces substantial impairment of physical or physiologic function, or involves extensive tissue dissection or transection”

47
Q

Define minor surgery

A

“does not expose a body cavity and causes little or no physical impairment”

48
Q

True or false: aseptic techique in rodents requires the use of a sterile gown and gloves.

A

False, but tissue handling should be done with sterile istrument tips

49
Q

What is the most appropriate method for monitoring depth of anesthesia in rodents?

A

Toe pinch test –firm pinch of the foot or toe should not elicit any movement in an adequately anesthetized animal

50
Q

Which rodent spp should not have anesthetic depth assessed by the toe pinch method?

A

Guinea pigs, toe pinch reflex present at surgical planes of anesthesia

51
Q

What is the grimace test in mice?

A

A pain assessment tool