MHC Antigen Processing (Exam 2) Flashcards

1
Q

Term for having more than one allele for a gene.

A

polymorphism

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2
Q

_______ are the most polymorphic genes known with some having greater than 250 different alleles.

A

MHC genes

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3
Q

Term for MHC having different class I and II genes so each individual has a different set of MHC molecule with unique peptide-binding specificity.

A

polygenism

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4
Q

Term for all genes on MHC molecules being equally expressed.

A

co-dominance

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5
Q

Term for when individuals express same alleles for a gene.

A

syngeneic

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6
Q

Term for when individuals express at least one different allele.

A

allogeneic

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7
Q

What is an allele?

A

variation of a gene at the same place on a chromosome

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8
Q

List the symbols used for mouse MHC nomenclature for Class I genes, Class II genes, and alleles.

A

Class I: K, D, L
Class II: A, E
Alleles: lowercase letters (a, b, c)

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9
Q

What is the shorthand for Class I MHC molecule nomenclature for mice? Class II?

A

Class I: H-2
Class II: I-A

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10
Q

In the example H-2K^d, what do each symbol represent? Is it mouse or human MHC molecule?

A

Mouse
H-2: Class I MHC molecule
K: Class I gene
d: allele

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11
Q

List the symbols used for human MHC nomenclature for Class I genes, Class II genes, and alleles.

A

Class I: A, B, C
Class II: DP, DQ, DR
alleles: numbers (1,2,3)

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12
Q

What shorthand is used to identify a human MHC molecule?

A

HLA (human leukocyte antigen)

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13
Q

In the MHC molecule example, HLA-DR4, what do each symbol represent? Is it from a human or mouse?

A

human
HLA: human MHC molecule
DR: Class II gene
4: allele

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14
Q

In MHC restriction, T cells from an individual can only respond to antigen if it recognizes what two things?

A
  1. peptide
  2. MHC molecule
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15
Q

Term for a set of MHC alleles for a given individual.

A

MHC haplotype

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16
Q

Term for when T cells must recognize both a peptide and its bound MHC molecule to create a response.

A

MHC restriction

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17
Q

In terms of Class I MHC structure, it is made up of a _______ and ______.

A

B2-microglobulin
alpha chain

18
Q

Class II MHC molecules are made up of _______ and ______.

A

half alpha + half beta chain

19
Q

To be considered fully assembled, a Class I MHC molecule needs to have these 3 things:

A
  1. alpha chain
  2. B2-microglobulin
  3. bound peptide
20
Q

Class I MHC are expressed on ________ cells while Class II MHC are expressed on ________ cells.

A

nucleated
antigen-presenting

21
Q

Peptides that bind to Class I MHC come from (inside/outside) the cell while for Class II MHC come from (inside/outside) the cell.

A

inside
outside

22
Q

Which CD on T cells do Class I and Class II MHC molecules bind?

A

Class I: CD8
Class II: CD4

23
Q

Where does peptide bind on MHC molecules?

A

peptide-binding cleft

24
Q

What structures of peptide side chains allow for tight and specific binding to peptide-binding cleft on MHC molecules?

A

anchor residues

25
Q

T cells recognize antigen only as _____.

A

short linear peptides

26
Q

(T/F) T cells only recognize antigens when they are presented to them on MHC as peptides.

A

True

27
Q

Term for a microbial product that enhances antigen presentation, as used in vaccines to improve their effectiveness.

A

adjuvant

28
Q

What is meant by a “professional antigen-presenting cell”?

A

express class II MHC molecules and present antigen to CD4 T cells

29
Q

Regarding the process of antigen capture and presentation, _________ capture antigen and activated ________ travel through lymphatics to present antigen to _______.

A

tissue-resident dendritic cells
dendritic cells
T cells

30
Q

Cross-presentation is a specific antigen-processing pathway in (class I/class II) MHC molecules since you need to activate (CD4/CD8) cells.

A

Class I
CD8

31
Q

Explain cross-presentation.

A

dendritic cells sample antigen that is degraded and released in the cytosol so Class I MHC can bind to it and go to cell surface to activate CD8 cells

32
Q

Fill in the appropriate blanks for Class I MHC antigen-processing pathway.

  1. Antigen enters cell as ______.
  2. A _______ tag targets antigen for degradation into peptides.
  3. Peptides are transported into ER via _____.
  4. Peptides bind to Class I MHC.
  5. MHC molecules are transported out via _______ and onto cell surface.
A

cytosolic protein
ubiquitin
TAP (transport associated protein)
golgi vesicles

33
Q

Fill in the appropriate blanks for Class II MHC antigen-processing pathway.

  1. Antigens enters cell as ________.
  2. ______ breaks antigen –> peptides.
  3. In ER, _______ is added to class II molecules.
A

endocytic vesicles
lysosome
invariant chain

34
Q

What is the purpose of the invariant chain?

A

prevent Class II MHC from binding to other peptides in ER and guides it to vesicles with appropriate peptides

35
Q

Once outside of the ER, the invariant chain on Class II MHC is broken down except _______.

A

CLIP

36
Q

What molecule removes CLIP from Class II MHC so a peptide can bind?

A

HLA-DM

37
Q

Term for membrane-bound vesicles in cytosol that carry antigen in the Class II MHC antigen-presenting pathway.

A

endosome

38
Q

(T/F) CLIP is easily degraded from Class II MHC molecules.

A

False

39
Q

_______ is a protein complex that degrades proteins into peptides for the Class I MHC pathway.

A

proteasome

40
Q

Term for when a specific peptide of a degraded antigen has a better affinity for MHC molecule which causes most T cells to respond to that one epitope and undergo clonal expansion.

A

immunodominant epitopes