Methods of Studying Cells Flashcards

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1
Q

Name the 3 types of microscope

A

Light Microscope
Scanning Electron Microscope
Transmission Electron
Microscope

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2
Q

define magnification

A

Magnifi cation is how much bigger the image is compared to the original object viewed with the naked eye

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3
Q

Magnification formula

A

(size of image)/(size of object)

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4
Q

define resolution

A

how well a microscope distinguishes between two

points that are close together.

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5
Q

what does Cell fractionation do?

A

separate organelles.

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6
Q

describe the types of Cell fractionation

A

Homogenisation - grinding cells release the organelles into solution
Filtration - separates organelles & debris
Ultracentrifugation - using a centrifuge the organelles are
separated out in order of mass

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7
Q

3 types of a microscope - medium

A

Light Microscope - light beam
Scanning Electron Microscope - electron beam
Transmission Electron
Microscope - electron beam

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8
Q

3 types of a microscope - dimensions

A

Light Microscope - 2D
Scanning Electron Microscope - 3D
Transmission Electron
Microscope - 2D

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9
Q

3 types of a microscope - in order of increasing magnitude and best resolution

A

Light Microscope
Scanning Electron Microscope
Transmission Electron
Microscope

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