Methods of Studying Cells Flashcards
Name the 3 types of microscope
Light Microscope
Scanning Electron Microscope
Transmission Electron
Microscope
define magnification
Magnifi cation is how much bigger the image is compared to the original object viewed with the naked eye
Magnification formula
(size of image)/(size of object)
define resolution
how well a microscope distinguishes between two
points that are close together.
what does Cell fractionation do?
separate organelles.
describe the types of Cell fractionation
Homogenisation - grinding cells release the organelles into solution
Filtration - separates organelles & debris
Ultracentrifugation - using a centrifuge the organelles are
separated out in order of mass
3 types of a microscope - medium
Light Microscope - light beam
Scanning Electron Microscope - electron beam
Transmission Electron
Microscope - electron beam
3 types of a microscope - dimensions
Light Microscope - 2D
Scanning Electron Microscope - 3D
Transmission Electron
Microscope - 2D
3 types of a microscope - in order of increasing magnitude and best resolution
Light Microscope
Scanning Electron Microscope
Transmission Electron
Microscope