Biochemical Tests Flashcards
1
Q
Test for Reducing sugars
A
Benedict’s reagent → Heat \+ red/ orange precipitate OR Reagent test strip \+compare with a calibration card
2
Q
What are reducing sugars?
A
A sugar that is capable of acting as a reducing agent because it has a free aldehyde group or a free ketone group.
All monosaccharides are reducing sugars, along with some disaccharides
3
Q
Test for non-reducing sugars
A
Hydrochloric acid → Heat
Sodium hydrogen carbonate
Benedict’s reagent → Heat
+ red/ orange precipitate
4
Q
Test for starch
A
Iodine in potassium iodide
solution
+Blue/black
5
Q
Test for proteins
A
Sodium hydroxide
Copper (II) sulphate
+ purple
6
Q
What are non-reducing sugars?
A
a carbohydrate that is not oxidized by a weak oxidizing agent
7
Q
What is starch?
A
Starch is a polysaccharide comprising glucose monomers joined in α 1,4 linkages
8
Q
test for lipids
A
Ethanol
Water → Shake
+ Cloudy white
