Media/pH Indicators

Question 1

Broth or Solid?

Answer 1

Presence of Agar

Question 2

Synthetic vs Non-Synthetic

Answer 2

Synthetic (+): Completely made up of chemical products
Non-Synthetic(-): At least ONE organic ingredient (proteins, animal/plant extracts, peptone, tryptone)

Question 3

Enriched Media

Answer 3

+: blood, serum, yeast extract, brain infusion

Question 4

Reducing Media

Answer 4

+: thioglycollate, thiosulfate, cysterine, cystine

Question 5

Buffering Media

Answer 5

+: 2 different phosphates

Question 6

Selective and Differential Media

Answer 6

+: salt, high/low pH, dyes, narrow spectrum abx, disinfectants, antimicrobials

Question 7

Phenol Red

Answer 7

Acidic: yellow </=6.8
Neutral: red 6.9-8.0
Alkaline: magenta (hot pink) >/= 8.0

Question 8

Litmus

Answer 8

Acidic: pink
Neutral: purple
Alkaline: blue

Question 9

Brom Cresol Purple

Answer 9

acidic: yellow
Neutral: burgundy
Alkaline: violet

Question 10

Methyl Red:

Answer 10

Very acidic: Red </= 4.4
Acidic: orange 5-6
Neutral: yellow >/=6.3