Major Groups of Disease Causing Bacteria- RA2 Flashcards
Aerobic Gram (+) Rods
Bacillus (Forms spores)
Cornyebacterium (Chinese Characters)
Listeria
Anaerobic Spore-Forming Gram (+) Rods
Clostridium (Forms spores & some species have tennis racket appearance)
Gram (+) Cocci
Staphylococcus
Streptococcus
Enterococcus
Enteric Gram (-) Rods
Escherichia Campylobacter Yernisia enterocolitica Salmonella Shingella Helicobacter Vibrio Bacteroides
Non-enteric Gram (-) Rods
Haemophilus Legionella Francisella Pasteurella Brucella Rickettsia Pseudomonas Klebseilla Yernisia pestis
Gram (-) Cocci
Neisseria
Elementary/ Reticulate Body
Chlamydia
Chlamydophila
Spirochetes
Borrelia
Treponema
No Cell-Wall
Mycoplasma
Unique Cell Wall
Mycobacterium
Which Gram (+) cocci are catalase (+) & which are catalase (-)?
Staphylococcus is Catalase (+)
Streptococcus & Enterococcus are Catalase (-)
You are attempting to classify a Gram (+) cocci & have determined it to be Catalase (+). What type of Gram (+) Cocci is this? What is the next step?
- Staphylococcus
- Coagulase Test
What will the a positive Coagulase Test tell you?
Positive= staphylococcus aureus
The Gram (-) Cocci in question is Coagulase negative. What is the next step?
Novobiocin Test
What will the Novobiocin Test tell you?
Sensitive= Staphylococcus epidermidis Resistant= Staphylococcus saprophyticus
You are attempting to classify a Gram (+) cocci & have determined it to be Catalase (-). What type of Gram (+) Cocci could this be?
Either Streptococcus or Enterococcus
What is the next step after determining that the Gram (+) Cocci is Catalase (-)?
Hemolysis on Blood Agar Test
What are the three possible results of a Hemolysis on Blood Agar Test?
Alpha
Beta
Gamma
The Gram (+) Cocci is gamma on the Hemolysis on Blood Agar Test. What do you now know?
This is an Enterococcus species
After determining that the Gram (+) Cocci in question is alpha on Hemolysis on Blood Agar Test, what is the next step?
Optochin Sensitivity Test
What species is sensitive on Optochin Sensitivity Test?
Streptococcus pneumoniae
What species is resistant on Optochin Sensitivity Test?
Enterococcus species
After determining that the Gram (+) Cocci in question is beta on Hemolysis on Blood Agar Test, what is the next step?
Bacitracin Sensitivity Test
What species is sensitive on Bacitracin Sensitivity Test?
Streptococcus pyogens
What species is resistant on Bacitracin Sensitivity Test?
Streptococcus agalactiae
You are attempting to determine the genera of a Gram (+) Rod. You perform the Anaerobic Growth Test & it is positive. What is the bacterium?
Clostridium species
You are attempting to determine the genera of a Gram (+) Rod. You perform the Anaerobic Growth Test and it is negative. What is the next step?
Spore Formation Test
A Gram (+) Rod is Anaerobic Growth Test (-). You perform the Spore Formation Test & it is positive. What genera must this bacterium be?
Bacillus
How would you differentiate between Bacillus species if a spore formation test was positive?
Motility Test
A Gram (+) Rod is Anaerobic Growth Test (-), Spore Formation Test (+), & Motility Test (+). What species is it?
Bacillus cereus
A Gram (+) Rod is Anaerobic Growth Test (-) & Spore Formation Test (-). What genera could it be?
Listeria or Corynebacterium
A Gram (+) Rod is Anaerobic Growth Test (-) & Spore Formation Test (-). How would you determine what species it is?
Motility Test
A Gram (+) Rod is Anaerobic Growth Test (-) & Spore Formation Test (-). You perform the Motility Test & it is positive. What bacteria is this?
Listeria monocytogenes
A Gram (+) Rod is Anaerobic Growth Test (-) & Spore Formation Test (-). You perform the Motility Test & it is negative. What bacteria is this?
Corynebacterium diptheriae
A Neisseria species is Maltose Test (+). What species is it?
Neisseria meningitidis
A Neisseria species is Maltose Test (-). What species is it?
Neiseria gonorrhoeae
You are attempting to classify Enteric Gram (-) Rods. What is the first test that you should perform?
Lactose Fermentation Test?
You are attempting to classify Enteric Gram (-) Rods & perform a Lactose Fermentation Test, which is positive. What could this bacteria be?
Escherichieae coli
Klebsiella pneumoniae*
You are attempting to classify Enteric Gram (-) Rods & perform a Lactose Fermentation Test, which is negative. What is the next step?
H2 Production Test/ Motility Test
You are attempting to classify Enteric Gram (-) Rods & perform a Lactose Fermentation Test; the test is negative. Next you perform a H2 Production Test/Motility Test, which is positive. What bacteria could is this be?
Salmonella enterica
Salmonella typhi
Proteus species
You are attempting to classify Enteric Gram (-) Rods & perform a Lactose Fermentation Test; the test is negative. Next you perform a H2 Production Test/Motility Test, which is negative. What bacteria could is this be?
Shigella species
Yernisia pestis*
Chlamydia & Chlamydophila
Obligate parasites
Borrelia
Wright’s Staining
Mycobacteria
Acid Fast Staining
Mycoplasma
Lack cell wall